COL2A1 ELISA Kit

Catalog No. ABIN5674675

Product Details COL2A1 ELISA Kit

Target: COL2A1 (Collagen, Type II, alpha 1 (COL2A1))

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.156 ng/mL - 10 ng/mL

Minimum Detection Limit: 0.156 ng/mL

Application: ELISA

Sample Type: Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Sensitivity: 0055 ng/mL

Components: Pre-coated, ready to use 96-well strip plate
Standard (freeze dried)
Standard Diluent
Detection Reagent A
Detection Reagent B
Assay Diluent A
Assay Diluent B
TMB Substhumane
Stop Solution
Wash Buffer(30 x concenthumane)
Plate sealer for 96 wells
Instruction manual

Material not included: 1. Microplate reader with 450 ± 10nm filter.
2. Precision single or multi-channel pipettes and disposable tips.
3. Eppendorf Tubes for diluting samples.
4. Deionized or distilled water.
5. Absorbent paper for blotting the microtiter plate.
6. Container for Wash Solution.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Assay Time: 1 - 4.5 h

Plate: Pre-coated

Protocol: 1. Prepare all reagents, samples and standards
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37℃
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37℃
4. Aspirate and wash 3 times
5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37℃
6. Aspirate and wash 5 times
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37℃
8. Add 50µL Stop Solution. Read at 450nm immediately.

Restrictions: For Research Use only

Handling

Storage: 4 °C

Expiry Date: 12 months

Target Details for COL2A1

Target: COL2A1 (Collagen, Type II, alpha 1 (COL2A1))

Alternative Name: CollagenType II Alpha 1 (COL2a1) (COL2A1 Products)

Synonyms: ANFH ELISA Kit, AOM ELISA Kit, COL11A3 ELISA Kit, SEDC ELISA Kit, Col2 ELISA Kit, Col2a ELISA Kit, Col2a-1 ELISA Kit, Del1 ELISA Kit, Dmm ELISA Kit, Lpk ELISA Kit, M100856 ELISA Kit, Rgsc856 ELISA Kit, CG2A1A ELISA Kit, COLLII ELISA Kit, col2a1a ELISA Kit, CB11 ELISA Kit, col2a1 ELISA Kit, coll2a1 ELISA Kit, fb38c06 ELISA Kit, fc10c01 ELISA Kit, wu:fb38c06 ELISA Kit, wu:fc10c01 ELISA Kit, collagen type II alpha 1 chain ELISA Kit, collagen, type II, alpha 1 ELISA Kit, collagen, type II, alpha 1 L homeolog ELISA Kit, collagen, type II, alpha 1 S homeolog ELISA Kit, collagen, type II, alpha 1a ELISA Kit, COL2A1 ELISA Kit, Col2a1 ELISA Kit, col2a1 ELISA Kit, col2a1.L ELISA Kit, col2a1.S ELISA Kit, col2a1a ELISA Kit

Background: Alternative name: COL2-A1, COL2A1, SEDC, Chondrocalcin, Collagen Alpha-1(II)Chain, Primary Osteoarthritis,Spondyloepiphyseal Dysplasia,Congenital

Gene ID: 12824

UniProt: P28481

Pathways: Sensory Perception of Sound, Growth Factor Binding