EDNRB ELISA Kit

Catalog No. ABIN5690869

Product Details EDNRB ELISA Kit

Target: EDNRB (Endothelin Receptor Type B (EDNRB))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Application: ELISA

Purpose: Human EDNRB/ET-BR ELISA Kit.

Sample Type: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Specificity: This ELISA antibody pair recognizes Human EDNRB.

Characteristics:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Components:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Material not included:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Application Details

Plate: Pre-coated

Protocol:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Handling

Storage: 4 °C

Expiry Date: 6 months

Target Details for EDNRB

Target: EDNRB (Endothelin Receptor Type B (EDNRB))

Alternative Name: EDNRB (ET-BR (EDNRB Products)

Synonyms: ABCDS ELISA Kit, ET-B ELISA Kit, ET-BR ELISA Kit, ETB ELISA Kit, ETBR ELISA Kit, ETRB ELISA Kit, HSCR ELISA Kit, HSCR2 ELISA Kit, WS4A ELISA Kit, Ednra ELISA Kit, Etb ELISA Kit, ETR-b ELISA Kit, ETb ELISA Kit, Sox10m1 ELISA Kit, EDNRB ELISA Kit, abcds ELISA Kit, etb ELISA Kit, etrb ELISA Kit, hscr ELISA Kit, hscr2 ELISA Kit, endothelin receptor type B ELISA Kit, EDNRB ELISA Kit, Ednrb ELISA Kit, ednrb ELISA Kit

Gene ID: 1910

UniProt: P24530

Pathways: Cellular Response to Molecule of Bacterial Origin, cAMP Metabolic Process