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TYRO3 ELISA Kit

TYRO3 Reactivity: Human Colorimetric Sandwich ELISA 40-10,000 pg/mL Cell Culture Supernatant, Serum
Catalog No. ABIN625289
  • Target See all TYRO3 ELISA Kits
    TYRO3 (TYRO3 Protein Tyrosine Kinase (TYRO3))
    Reactivity
    • 4
    • 4
    • 2
    • 1
    • 1
    Human
    Detection Method
    Colorimetric
    Method Type
    Sandwich ELISA
    Detection Range
    40-10,000 pg/mL
    Minimum Detection Limit
    40 pg/mL
    Application
    ELISA
    Purpose
    Human Dtk ELISA Kit for cell culture supernatants, plasma, and serum samples.
    Sample Type
    Cell Culture Supernatant, Serum
    Analytical Method
    Quantitative
    Characteristics
    Custom Human Dtk ELISA Kit available on demand
    Components
    Pre-Coated 96-Well Strip Microplate
    Wash Buffer
    Stop Solution
    Assay Diluent(s)
    Lyophilized Standard
    Biotinylated Detection Antibody
    Streptavidin-Conjugated HRP
    TMB One-Step Substrate
    Material not included
    Distilled or deionized water
    100 mL and 1 liter graduated cylinders
    Tubes to prepare sample dilutions
    Protease and Phosphatase inhibitors
    Precision pipettes to deliver 2 μL to 1 mL volumes
    Adjustable 1-25 mL pipettes for reagent preparation
    Benchtop rocker or shaker
    Microplate reader capable of measuring absorbance at 450 nm
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  • Sample Volume
    100 μL
    Plate
    Pre-coated
    Protocol
    Prepare all reagents, samples and standards as instructed in the manual.
    Add 100 µl of standard or sample to each well.
    Incubate 2.5 h at RT or O/N at 4°C.
    Add 100 µl of prepared biotin antibody to each well.
    Incubate 1 h at RT.
    Add 100 µl of prepared Streptavidin solution to each well.
    Incubate 45 min at RT.
    Add 100 µl of TMB One-Step Substrate Reagent to each well.
    Incubate 30 min at RT.
    Add 50 µl of Stop Solution to each well.
    Read at 450 nm immediately.
    Restrictions
    For Research Use only
  • Storage
    4 °C
    Expiry Date
    6 months
  • Target See all TYRO3 ELISA Kits
    TYRO3 (TYRO3 Protein Tyrosine Kinase (TYRO3))
    Alternative Name
    Dtk (TYRO3 Products)
    Synonyms
    TYRO3 ELISA Kit, brt ELISA Kit, byk ELISA Kit, dtk ELISA Kit, rse ELISA Kit, sky ELISA Kit, tif ELISA Kit, Xksy ELISA Kit, MGC145734 ELISA Kit, DKFZp469I0432 ELISA Kit, LOC100231598 ELISA Kit, BYK ELISA Kit, Dtk ELISA Kit, RSE ELISA Kit, Sky ELISA Kit, Tif ELISA Kit, Brt ELISA Kit, REK ELISA Kit, ksy ELISA Kit, xksy ELISA Kit, AI323366 ELISA Kit, Etk-2 ELISA Kit, Rse ELISA Kit, TYRO3 protein tyrosine kinase ELISA Kit, TYRO3 protein tyrosine kinase L homeolog ELISA Kit, TYRO3 protein tyrosine kinase 3 ELISA Kit, TYRO3 ELISA Kit, tyro3 ELISA Kit, Tyro3 ELISA Kit, tyro3.L ELISA Kit
    Pathways
    RTK Signaling
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