FLT3 ELISA Kit (Fms-Related tyrosine Kinase 3)

Details for Product FLT3 ELISA Kit No. ABIN6730594
Antigen
  • FLT3
  • B230315G04
  • CD135
  • Flk-2
  • Flk2
  • Flt-3
  • Ly72
  • wmfl
  • FLK-2
  • FLK2
  • STK1
  • fms related tyrosine kinase 3
  • FMS-like tyrosine kinase 3
  • fms-related tyrosine kinase 3
  • FLT3
  • Flt3
Epitope
pTyr589
Reactivity
Human
Alternatives
Kits with alternative reactivity to:
7
5
2
1
1
Detection Method
Colorimetric
Method Type
Sandwich ELISA
Application
ELISA
Options
Purpose Human Phospho-FLT3 (Tyr589) and Total FLT3 ELISA Kit. This assay semi-quantitatively measures FLT3 phosphorylated at Tyrosine-589 as well as total FLT3 in cell lysate samples.
Sample Type Cell Samples, Tissue Lysate
Analytical Method Semi-Quantitative
Detection Method Colorimetric
Specificity This ELISA kit recognizes Human FLT3 phosphorylated at site Tyrosine-589 as well as total FLT3.
Characteristics
  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Anti-Phospho Antibody
  • Anti-Pan Antibody
  • HRP-Conjugated Secondary Antibody
  • Streptavidin-Conjugated HRP
  • Assay Diluent
  • TMB One-Step Substrate
  • Stop Solution
  • Lysis Buffer
  • Positive Control Sample
Components
  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Anti-Phospho Antibody
  • Anti-Pan Antibody
  • HRP-Conjugated Secondary Antibody
  • Streptavidin-Conjugated HRP
  • Assay Diluent
  • TMB One-Step Substrate
  • Stop Solution
  • Lysis Buffer
  • Positive Control Sample
Material not included
  • Distilled or deionized water
  • 100 mL and 1 liter graduated cylinders
  • Tubes to prepare sample dilutions
  • Protease and Phosphatase inhibitors
  • Precision pipettes to deliver 2 μL to 1 mL volumes
  • Adjustable 1-25 mL pipettes for reagent preparation
  • Benchtop rocker or shaker
  • Microplate reader capable of measuring absorbance at 450 nm
Plasmids, Primers & others Plasmids, Primers & others FLT3 products on genomics-online (e.g. as negative or positive controls)
Antigen
Alternative Name FLT3 (FLT3 ELISA Kit Abstract)
Gene ID 2322
UniProt P36888
Pathways RTK Signaling
Application Notes Optimal working dilution should be determined by the investigator.
Protocol
  1. Prepare all reagents and samples as instructed in the manual.
  2. Add 100 μL of sample or positive control to each well.
  3. Incubate 2.5 h at RT or O/N at 4 °C.
  4. Add 100 μL of prepared primary antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 μL of prepared 1X HRP-Streptavidin to each well.
  7. Incubate 1 h at RT.
  8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 μL of Stop Solution to each well.
  11. Read at 450 nm immediately.
Restrictions For Research Use only
Storage -20 °C
Storage Comment Upon receipt, the kit should be stored at -20 °C. Please use within 6 months from the date of shipment. After initial use, Wash Buffer Concentrate (Item B), Assay Diluent (Item E), TMB One-Step Substrate Reagent (Item H), HRP-Streptavidin (Item G), Stop Solution (Item I) and Cell Lysate Buffer (Item J) should be stored at 4 °C to avoid repeated freeze-thaw cycles. Return unused wells to the pouch containing desiccant pack, reseal along entire edge and store at -20 °C. Reconstituted Positive Control (Item K) should be stored at -70 °C.
Expiry Date 6 months
Supplier Images
Image no. 1 for Fms-Related tyrosine Kinase 3 (FLT3) ELISA Kit (ABIN6730594) Jurkat cells were treated with Pervanadate. Solubilize cells at 4 x 10^7 cells/ml in ...
Image no. 2 for Fms-Related tyrosine Kinase 3 (FLT3) ELISA Kit (ABIN6730594) A431 cells were treated or untreated with Pervanadate. Cell lysates were analyzed usi...
Image no. 3 for Fms-Related tyrosine Kinase 3 (FLT3) ELISA Kit (ABIN6730594) A431 cells were treated or untreated with Pervanadate. Cell lysates were analyzed usi...
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