IKBKB ELISA Kit

Catalog No. ABIN6951455

Product Details IKBKB ELISA Kit

Target: IKBKB (Inhibitor of kappa Light Polypeptide Gene Enhancer in B-Cells, Kinase beta (IKBKB))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Application: ELISA

Purpose: Human IKK-B ELISA Kit.

Sample Type: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Characteristics:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Components:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Material not included:

• Distilled or deionized water
• Precision pipettes to deliver 2 µl to 1 µl volumes
• Adjustable 1-25 µl pipettes for reagent preparation
• 100 µl and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Plate: Pre-coated

Protocol:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Handling

Expiry Date: 6 months

Target Details for IKBKB

Target: IKBKB (Inhibitor of kappa Light Polypeptide Gene Enhancer in B-Cells, Kinase beta (IKBKB))

Alternative Name: IKK-B (IKBKB Products)

Synonyms: ikk-beta ELISA Kit, ikk2 ELISA Kit, ikkb ELISA Kit, ikkbeta ELISA Kit, IKBKB ELISA Kit, IKKB ELISA Kit, CG4201 ELISA Kit, DIK ELISA Kit, DLAK ELISA Kit, DmIKK ELISA Kit, DmIKKBeta ELISA Kit, DmIKKb ELISA Kit, DmIKKbeta ELISA Kit, DmIkkbeta ELISA Kit, Dmel\\CG4201 ELISA Kit, Dmikkb ELISA Kit, Dmikkbeta ELISA Kit, IK ELISA Kit, IKK ELISA Kit, IKK-beta ELISA Kit, IKKb ELISA Kit, IKKbeta ELISA Kit, IRD-5 ELISA Kit, IRD5 ELISA Kit, Ik ELISA Kit, Ikkb ELISA Kit, Ikkbeta ELISA Kit, Ird-5 ELISA Kit, Ird5 ELISA Kit, LRK ELISA Kit, Lak ELISA Kit, anon-89Bd ELISA Kit, dIKK ELISA Kit, dIKK-beta ELISA Kit, dIKKbeta ELISA Kit, dLak ELISA Kit, dmIKKbeta ELISA Kit, ikk ELISA Kit, ird ELISA Kit, ird5/DmIkk[beta] ELISA Kit, IKK2 ELISA Kit, NFKBIKB ELISA Kit, AIM-1 ELISA Kit, AI132552 ELISA Kit, IKK-2 ELISA Kit, IKK[b] ELISA Kit, inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase beta L homeolog ELISA Kit, inhibitor of nuclear factor kappa B kinase subunit beta ELISA Kit, I-kappaB kinase beta ELISA Kit, inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase beta ELISA Kit, inhibitor of kappaB kinase beta ELISA Kit, ikbkb.L ELISA Kit, IKBKB ELISA Kit, ikbkb ELISA Kit, IKKbeta ELISA Kit, Ikbkb ELISA Kit

Gene ID: 3551

UniProt: O14920

Pathways: NF-kappaB Signaling, RTK Signaling, TCR Signaling, TLR Signaling, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Production of Molecular Mediator of Immune Response, Hepatitis C, Toll-Like Receptors Cascades, BCR Signaling, Ubiquitin Proteasome Pathway, S100 Proteins