p53 ELISA Kit

Catalog No. ABIN8071210

The Human, Rat and Mouse p53 ELISA Kit (ABIN8071210) is a Colorimetric ELISA Kit designed to quantify Human, Rat and Mouse p53.

Quick Overview for p53 ELISA Kit (ABIN8071210)

Target: p53 (TP53) (Tumor Protein P53 (TP53))

Binding Specificity: ubiquitinated

Reactivity: Human, Rat, Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.3125 ng/mL - 20 ng/mL

Application: ELISA

Sample Type: Cell Lysate, Tissue Lysate

Product Details p53 ELISA Kit

Minimum Detection Limit: 0.3125 ng/mL

Purpose: Ubiquitinated p53 Quantitative ELISA kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human, Mouse, Rat Ubiquitinated p53.

Analytical Method: Quantitative

Specificity: This ELISA antibody pair detects Human, Mouse, Rat Ubiquitinated p53.

Sensitivity: 0.07 ng/mL

Components:

Size / Qty
• Anti-Pan-p53 Microplate
• 96 wells
• Ubiquitinated-p53 Standard
• Anti-Ubiquitin Detection Antibody
• HRP-Streptavidin
• 200 μL
• Wash Buffer
• Assay Diluent B
• Lysis Buffer
• TMB One-Step Substrate Reagent
• Stop Solution

Material not included:

• Microplate reader capable of measuring absorbance at 450 nm
• Protease and Phosphatase inhibitors
• Precision pipettes to deliver 2 μL to 1 mL volumes
• Adjustable 1-25 mL pipettes for reagent preparation
• 100 mL and 1 liter graduated cylinders
• Log-log graph paper or computer and software for ELISA data analysis
• Absorbent paper
• Tubes to prepare positive control or sample dilutions
• Distilled or deionized water
• Shaker

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Plate: Pre-coated

Protocol:

1. Prepare all reagents, samples and standards as instructed.
2. Add 100 μL of each standard and sample to each well. Incubate 2.5 hours at room temperature or overnight at 4 °C with gentle shaking.
3. Add 100 μL prepared detection antibody to each well. Incubate for 1 hour at room temperature with gentle shaking.
4. Add 100 μL prepared HRP-Streptavidin solution. Incubate for 1 hour at room temperature with gentle shaking.
5. Add 100 μL TMB One-Step Substrate Reagent to each well. Incubate 30 minutes at room temperature.
6. Add 50 μL Stop Solution to each well. Read at 450 nm immediately.

Restrictions: For Research Use only

Handling

Storage: -20 °C

Storage Comment: The entire kit may be stored at -20°C for up to 6 months from the date of shipment. Avoid repeated freeze-thaw cycles.

Target Details for p53

Target: p53 (TP53) (Tumor Protein P53 (TP53))

Alternative Name: p53

Background:

Gene Symbols: TP53 P53

Background: The Ubiquitinated p53 Quantitative ELISA kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human, Mouse, Rat Ubiquitinated p53. An anti-pan p53 antibody has been coated onto a 96-well plate. Standard and Samples are pipetted into the wells and p53 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-ubiquitin antibody is used to detect ubiquitinated p53. After washing away unbound antibody, HRP-conjugated streptavidin is pipetted into the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of ubiquitinated p53 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Protein Name (Synonyms): Cellular tumor antigen p53 (Antigen NY-CO-13) (Phosphoprotein p53) (Tumor suppressor p53)

Gene ID: 71572205924842

UniProt: P04637, P02340, P10361

Pathways: p53 Signaling, MAPK Signaling, PI3K-Akt Signaling, Apoptosis, AMPK Signaling, Chromatin Binding, ER-Nucleus Signaling, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Autophagy, Warburg Effect