p53 CLIA Kit
Quick Overview for p53 CLIA Kit (ABIN8088631)
Target
See all p53 (TP53) CLIA KitsReactivity
Detection Method
Method Type
Detection Range
Application
Sample Type
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Minimum Detection Limit
- 27.4 pg/mL
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Purpose
- Human Tumor Protein p53 (P53) CLIA Kit for detecting P53 in serum, plasma, tissue homogenates, cell lysates, cell culture supernates
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Analytical Method
- Quantitative
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Sensitivity
- 9.5 pg/mL
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Components
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- Pre-coated, ready to use 96-well strip plate
- Standar
- Detection Reagent A
- Detection Reagent B
- Substrate A
- Substrate B
- Assay Diluent A
- Assay Diluent B
- Wash Buffer (30x concentrate)
- Standard Diluent
- Plate Sealer
- Instruction Manual
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Material not included
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- Microplate Reader with 450 nm filter
- Single or multi-channel pipettes with high precision and disposable tips
- Microcentrifuge tubes
- Deionized or distilled watter
- Absorbent paper for blotting the microplate
- Container for wash solution
- 0.01 M (or 1x) Phosphate Buffered Saline (PBS), ph 7.0-7.2
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Tumor Protein P53 (TP53) ELISA Kit
TP53 Reactivity: Human Colorimetric Sandwich ELISA 9.38 pg/mL - 600 pg/mL Cell Culture Supernatant, Cell Lysate, Cerebrospinal Fluid, Serum, Tissue Homogenate, Urine
Tumor Protein P53 (TP53) ELISA KitTP53 Reactivity: Human Colorimetric Sandwich ELISA 78.125 pg/mL - 5000 pg/mL Plasma, Serum, Tissue Homogenate
Tumor Protein P53 (TP53) ELISA KitTP53 Reactivity: Human Colorimetric Sandwich ELISA 78.13 pg/mL - 5000 pg/mL Cell Culture Supernatant, Plasma, Serum
Tumor Protein P53 (TP53) ELISA KitTP53 Reactivity: Human Colorimetric Sandwich ELISA 78 pg/mL - 5000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Tumor Protein P53 (TP53) ELISA KitTP53 Reactivity: Rat Colorimetric Sandwich ELISA 78 pg/mL - 5000 pg/mL Plasma, Serum, Tissue Homogenate
Tumor Protein P53 (TP53) ELISA KitTP53 Reactivity: Mouse Colorimetric Sandwich ELISA 78 pg/mL - 5000 pg/mL Plasma, Serum
Tumor Protein P53 (TP53) ELISA KitTP53 Reactivity: Rat Colorimetric Sandwich ELISA 78.125 pg/mL - 5000 pg/mL Plasma, Serum, Tissue Homogenate
Tumor Protein P53 (TP53) ELISA KitTP53 Reactivity: Human acetylated Colorimetric Sandwich ELISA 46.875 pg/mL - 3000 pg/mL Plasma, Serum, Tissue Homogenate
Tumor Protein P53 (TP53) ELISA KitTP53 Reactivity: Mouse Colorimetric Sandwich ELISA 62.5 pg/mL - 4000 pg/mL Plasma, Serum, Tissue Homogenate
Tumor Protein P53 (TP53) ELISA KitTP53 Reactivity: Human Colorimetric DNA-Binding ELISA Cell Lysate, Nuclear Extract
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Application Notes
- Optimal working dilution should be determined by the investigator.
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Comment
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This product is tested and assembled before shipping to ensure that it meets its quality specifications. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.
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Sample Volume
- 100 μL
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Assay Time
- 3 h
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Plate
- Pre-coated
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Assay Precision
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Intra-Assay: CV<10%,
Inter-Assay: CV<12% -
Restrictions
- For Research Use only
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Storage
- 4 °C
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Storage Comment
- 4°C for one month, Long term: see manual.
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Expiry Date
- 12 months
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- p53 (TP53) (Tumor Protein P53 (TP53))
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Alternative Name
- Tumor Protein p53
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Background
- Gene Aliases: TP53, LFS1, TRP53, Li-Fraumeni Syndrome, Cellular tumor antigen p53, Antigen NY-CO-13, Phosphoprotein p53, Tumor suppressor p53
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Gene ID
- 7157
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UniProt
- P04637
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Pathways
- p53 Signaling, MAPK Signaling, PI3K-Akt Signaling, Apoptosis, AMPK Signaling, Chromatin Binding, ER-Nucleus Signaling, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Autophagy, Warburg Effect
Target See all p53 (TP53) CLIA Kits
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