Rabbit Peroxidase Anti-Peroxidase (PAP) Soluble Complex

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Labeling (Lbl)
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Immunogen Peroxidase (Horseradish)
Specificity This product was prepared from rabbit polyclonal antiserum by delipidation, salt fractionation and ion exchange chromatography. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum and anti-Peroxidase.
Background The peroxidase-antiperoxidase (PAP) approach utilizes a complex of enzyme and antibody to facilitate amplification of signal, indirectly detecting the amount of primary antibody used in an assay. Generated in Rabbit, the PAP complex can be utilized in any assay that utilizes a rabbit primary antibody. With the addition of a secondary antibody that recognizes rabbit, the reporter PAP complex allows for amplification of the luminescent signal (as three molecules of enzyme are bound to every two immunoglobulins).
Synonyms: Horseradish peroxidase; HRP
Application Notes Suitable for use in peroxidase enzymatic labeling experiments.

Detection Kit Type: PAP Kit

Restrictions For Research Use only
Format Lyophilized
Reconstitution Reconstitution Buffer: Restore with PBS + 5% normal bridging host serum
Concentration 5.0 mg/mL. Concentration Definition: by UV absorbance at 280 nm
Buffer 0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2.
Storage 4 °C