anti-Pig (Porcine) BCL2 antibody for Immunohistochemistry

Recommended BCL2 Antibody (supplied by: Log in to see )

Antigen
B-Cell CLL/lymphoma 2 (BCL2) Antibodies
  • Bcl-2
  • PPP1R50
  • bcl-2
  • AW986256
  • C430015F12Rik
  • D630044D05Rik
  • D830018M01Rik
  • BCL-2
  • Bcl2
  • BCL2, apoptosis regulator
  • B cell leukemia/lymphoma 2
  • B-cell CLL/lymphoma 2a
  • B-cell CLL/lymphoma 2 protein
  • BCL2
  • Bcl2
  • bcl2a
  • BCL-2
Epitope
AA 41-54
162
74
65
62
44
29
25
25
24
21
17
15
15
15
11
10
10
10
10
9
7
7
7
7
7
6
4
4
4
4
4
3
3
3
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Dog (Canine), Human, Monkey, Pig (Porcine)
1068
263
220
51
25
22
19
15
5
4
3
3
3
2
2
2
Host
Mouse
578
556
18
6
2
2
2
Clonality
Monoclonal
Conjugate
This BCL2 antibody is un-conjugated
52
51
45
41
35
25
24
16
15
15
14
14
14
14
14
14
13
13
7
7
7
6
6
6
6
6
6
6
2
2
1
1
1
1
1
1
1
1
1
1
1
Application
ELISA, Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
847
419
383
363
339
338
234
195
168
81
40
15
14
13
12
11
9
3
3
2
1
1
1
1
1
1
1
Supplier
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Catalog No. ABIN337080
$ 639.83
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN4283388 FACS ICC IF IHC IHC (fro) IHC (p) WB Mouse IgG1 kappa Log in to see 8C8 0
1 ABIN957155 IHC IHC (fro) IHC (p) IP WB Rabbit AA 41-54 Log in to see Polyclonal 0
1 ABIN4283406 ELISA FACS ICC IF IHC IHC (fro) IHC (p) IP WB HRP Mouse IgG1 kappa Log in to see 8C8 0
1 ABIN386113 FACS IF IHC IHC (p) WB Mouse IgG1 AA 41-54 Log in to see 8C8 0
1 ABIN4283386 FACS ICC IF IHC IHC (fro) IHC (p) WB Mouse IgG1 kappa Log in to see 8C8 0
1 ABIN4283400 ELISA FACS ICC IF IHC IHC (fro) IHC (p) IP WB Alexa Fluor 700 Mouse IgG1 kappa Log in to see 8C8 0
1 ABIN4283402 ELISA FACS ICC IF IHC IHC (fro) IHC (p) IP WB Biotin Mouse IgG1 kappa Log in to see 8C8 0
1 ABIN4283405 ELISA FACS ICC IF IHC IHC (fro) IHC (p) IP WB DyLight 488 Mouse IgG1 kappa Log in to see 8C8 0
1 ABIN4283387 FACS ICC IF IHC IHC (fro) IHC (p) WB Mouse IgG1 kappa Log in to see 8C8 0
1 ABIN4283397 ELISA FACS ICC IF IHC IHC (fro) IHC (p) IP WB Alexa Fluor 405 Mouse IgG1 kappa Log in to see 8C8 0
1 ABIN4283403 ELISA FACS ICC IF IHC IHC (fro) IHC (p) IP WB DyLight 650 Mouse IgG1 kappa Log in to see 8C8 0
1 ABIN4283399 ELISA FACS ICC IF IHC IHC (fro) IHC (p) IP WB Alexa Fluor 647 Mouse IgG1 kappa Log in to see 8C8 0
1 ABIN4283407 ELISA FACS ICC IF IHC IHC (fro) IHC (p) IP WB DyLight 755 Mouse IgG1 kappa Log in to see 8C8 0
1 ABIN4283411 ELISA FACS ICC IF IHC IHC (fro) IHC (p) IP WB DyLight 350 Mouse IgG1 kappa Log in to see 8C8 0
1 ABIN4283408 ELISA FACS ICC IF IHC IHC (fro) IHC (p) IP WB PerCP Mouse IgG1 kappa Log in to see 8C8 0
1 ABIN4283412 ELISA FACS ICC IF IHC IHC (fro) IHC (p) IP WB DyLight 405 Mouse IgG1 kappa Log in to see 8C8 0
1 ABIN4283398 ELISA FACS ICC IF IHC IHC (fro) IHC (p) IP WB Alexa Fluor 488 Mouse IgG1 kappa Log in to see 8C8 0
1 ABIN4283404 ELISA FACS ICC IF IHC IHC (fro) IHC (p) IP WB DyLight 680 Mouse IgG1 kappa Log in to see 8C8 0
1 ABIN4283410 ELISA FACS ICC IF IHC IHC (fro) IHC (p) IP WB DyLight 550 Mouse IgG1 kappa Log in to see 8C8 0
1 ABIN957156 IHC IHC (fro) IHC (p) IP WB Rabbit AA 41-54 Log in to see Polyclonal 0

General

Antigen B-Cell CLL/lymphoma 2 (BCL2) Antibodies
Epitope AA 41-54
(162), (74), (65), (62), (44), (29), (25), (25), (24), (21), (17), (15), (15), (15), (11), (10), (10), (10), (10), (9), (7), (7), (7), (7), (7), (6), (4), (4), (4), (4), (4), (3), (3), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Dog (Canine), Human, Monkey, Pig (Porcine)
(1068), (263), (220), (51), (25), (22), (19), (15), (5), (4), (3), (3), (3), (2), (2), (2)
Host Mouse
(578), (556), (18), (6), (2), (2), (2)
Clonality
Monoclonal
Conjugate This BCL2 antibody is un-conjugated
(52), (51), (45), (41), (35), (25), (24), (16), (15), (15), (14), (14), (14), (14), (14), (14), (13), (13), (7), (7), (7), (6), (6), (6), (6), (6), (6), (6), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application ELISA, Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(847), (419), (383), (363), (339), (338), (234), (195), (168), (81), (40), (15), (14), (13), (12), (11), (9), (3), (3), (2), (1), (1), (1), (1), (1), (1), (1)
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Product Details anti-BCL2 Antibody

Target Details BCL2 Application Details Handling Images
Brand IHC-plus™
Specificity Recognizes human Bcl-2a at ~25kD. Species cross-reactivity: monkey. Does not react with mouse or rat.
Predicted Reactivity Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Dog, Cat, Pig (100%) Bovine (93%) Horse (86%).
Purification Protein G purified
Immunogen Synthetic peptide corresponding to residues 41-54 (Cys-GAAPAPGIFSSQPG) of human bcl-2 protein. Cellular Localization: Cytoplasm. MW of antigen: 25-26kD. Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Dog, Cat, Pig (100%), Bovine (93%), Horse (86%).

Type of Immunogen: Synthetic peptide
Isotype IgG1

Target Details BCL2

Product Details anti-BCL2 Antibody Application Details Handling Images back to top
Antigen
Alternative Name BCL2 / Bcl-2 (BCL2 Antibody Abstract)
Background Name/Gene ID: BCL2
Family: Apoptosis

Synonyms: BCL2, Apoptosis regulator Bcl-2, B-cell CLL/lymphoma 2, B-cell lymphoma protein 2, Bcl-2, PPP1R50
Gene ID 596
UniProt P10415
Pathways MAPK Signaling, PI3K-Akt Signaling, Apoptosis, Caspase Cascade in Apoptosis, Regulation of Muscle Cell Differentiation, Cell-Cell Junction Organization, Skeletal Muscle Fiber Development, Autophagy, Smooth Muscle Cell Migration, Negative Regulation of intrinsic apoptotic Signaling

Application Details

Product Details anti-BCL2 Antibody Target Details BCL2 Handling Images back to top
Application Notes Approved: ELISA, Flo, IF, IHC, IHC-P (5 μg/mL), WB (1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. Western Blot: 1 μg/mL for 2hrs at RT. Positive control: Tonsil or follicular lymphomas. Raji or HeLa cells. The applications listed have been tested for the unmodified form of this product. Other forms have not been tested.
Comment

Target Species of Antibody: Human

Restrictions For Research Use only

Handling

Product Details anti-BCL2 Antibody Target Details BCL2 Application Details Images back to top
Format Liquid
Concentration Lot specific
Buffer PBS,  pH 7.4.
Preservative Azide free
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C,-20 °C
Storage Comment 4°C or -20°C, Avoid freeze-thaw cycles.

Images

Product Details anti-BCL2 Antibody Target Details BCL2 Application Details Handling back to top
Supplier Images
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-B-Cell CLL/lymphoma 2 (BCL2) (AA 41-54) antibody (ABIN337080) Human Spleen (formalin-fixed, paraffin-embedded) stained with BCL2 antibody ABIN33708...