anti-Xenopus laevis MAPK12 antibody for Western Blotting

Recommended MAPK12 Antibody (supplied by: Log in to see )

Antigen
Mitogen-Activated Protein Kinase 12 (MAPK12) Antibodies
  • ATMPK12
  • MAPK12
  • T3F17.28
  • mitogen-activated protein kinase 12
  • MGC160082
  • si:dkey-14d8.5
  • erk6
  • etID309866.18
  • mapk12
  • sapk3
  • wu:fa05c12
  • zgc:101695
  • ERK3
  • ERK6
  • P38GAMMA
  • PRKM12
  • SAPK-3
  • SAPK3
  • AW123708
  • Erk6
  • P38gamma
  • Prkm12
  • Sapk3
  • mitogen-activated protein kinase 12
  • mitogen-activated protein kinase 12b
  • mitogen-activated protein kinase 12a
  • MPK12
  • MAPK12
  • mapk12b
  • mapk12a
  • Mapk12
Epitope
AA 35-84
27
12
11
8
7
6
5
4
4
4
3
2
1
1
1
1
1
1
1
Reactivity
Cow (Bovine), Guinea Pig, Horse (Equine), Human, Monkey, Mouse (Murine), Pig (Porcine), Rabbit, Rat (Rattus), Xenopus laevis
110
49
20
4
4
3
3
2
2
2
1
1
Host
Rabbit
82
27
3
2
Clonality
Polyclonal
Conjugate
This MAPK12 antibody is un-conjugated
5
5
4
4
4
4
Application
Western Blotting (WB)
108
53
47
17
14
10
9
2
2
1
1
1
Supplier
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Catalog No. ABIN477368
$ 551.83
Plus shipping costs $45.00

General

Antigen Mitogen-Activated Protein Kinase 12 (MAPK12) Antibodies
Epitope AA 35-84
(27), (12), (11), (8), (7), (6), (5), (4), (4), (4), (3), (2), (1), (1), (1), (1), (1), (1), (1)
Reactivity Cow (Bovine), Guinea Pig, Horse (Equine), Human, Monkey, Mouse (Murine), Pig (Porcine), Rabbit, Rat (Rattus), Xenopus laevis
(110), (49), (20), (4), (4), (3), (3), (2), (2), (2), (1), (1)
Host Rabbit
(82), (27), (3), (2)
Clonality
Polyclonal
Conjugate This MAPK12 antibody is un-conjugated
(5), (5), (4), (4), (4), (4)
Application Western Blotting (WB)
(108), (53), (47), (17), (14), (10), (9), (2), (2), (1), (1), (1)
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Product Details anti-MAPK12 Antibody

Target Details MAPK12 Application Details Handling Images
Specificity Human MAPK12 / ERK5
Predicted Reactivity Percent identity by BLAST analysis: Human, Bovine, Rabbit, Horse, Pig, Guinea pig, Xenopus (100%) Dog, Zebrafish (92%).
Purification Immunoaffinity purified
Immunogen Synthetic peptide located between aa35-84 of human MAPK12 (P53778, NP_002960). Percent identity by BLAST analysis: Human, Gorilla, Monkey, Galago, Marmoset, Mouse, Rat, Panda, Bovine, Rabbit, Horse, Pig, Opossum, Guinea pig, Zebra finch, Xenopus, Catfish (100%), Gibbon (93%), Chimpanzee, Sheep, Elephant, Dog, Bat, Chicken, Platypus, Lizard, Salmon, Stickleback, Zebrafish (92%), Goat, Turkey (85%).

Type of Immunogen: Synthetic peptide
Isotype IgG

Target Details MAPK12

Product Details anti-MAPK12 Antibody Application Details Handling Images back to top
Antigen
Alternative Name MAPK12 / ERK6 / SAPK3 (MAPK12 Antibody Abstract)
Background Name/Gene ID: MAPK12
Subfamily: MAPK
Family: Protein Kinase

Synonyms: MAPK12, ERK5, ERK6, ERK-6, MAP kinase 12, MAPK 12, MAP kinase p38 gamma, SAPK3, p38-gamma, p38GAMMA, SAPK-3, ERK3, PRKM12
Gene ID 6300
NCBI Accession NP_002960
UniProt P53778
Pathways MAPK Signaling, Neurotrophin Signaling Pathway, Regulation of Muscle Cell Differentiation, Hepatitis C

Application Details

Product Details anti-MAPK12 Antibody Target Details MAPK12 Handling Images back to top
Application Notes Approved: WB (0.2 - 1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:62500.
Restrictions For Research Use only

Handling

Product Details anti-MAPK12 Antibody Target Details MAPK12 Application Details Images back to top
Format Lyophilized
Reconstitution Distilled water
Concentration Lot specific
Buffer Lyophilized from PBS with 2 % sucrose
Handling Advice Avoid repeat freeze-thaw cycles.
Storage 4 °C,-20 °C
Storage Comment Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than one week): +4°C. Avoid repeat freeze-thaw cycles.

Images

Product Details anti-MAPK12 Antibody Target Details MAPK12 Application Details Handling back to top
Supplier Images
 image for anti-Mitogen-Activated Protein Kinase 12 (MAPK12) (AA 35-84) antibody (ABIN477368) anti-Mitogen-Activated Protein Kinase 12 (MAPK12) (AA 35-84) antibody