anti-Sheep (Ovine) MAPK3 antibody for Immunofluorescence

Recommended MAPK3 Antibody (supplied by: Log in to see )

Antigen
Mitogen-Activated Protein Kinase 3 (MAPK3) Antibodies
  • ERK-1
  • ERK1
  • ERT2
  • HS44KDAP
  • HUMKER1A
  • P44ERK1
  • P44MAPK
  • PRKM3
  • p44-ERK1
  • p44-MAPK
  • Erk-1
  • Erk1
  • Ert2
  • Esrk1
  • Mnk1
  • Mtap2k
  • Prkm3
  • p44
  • p44erk1
  • p44mapk
  • fi06b09
  • wu:fi06b09
  • zERK1
  • Tb08.10J17.940
  • MAPK1
  • MNK1
  • ATMAPK3
  • ATMPK3
  • T6D9.4
  • mitogen-activated protein kinase 3
  • mitogen-activated protein kinase 3
  • mitogen activated protein kinase 3
  • mitogen-activated serine/threonine-protein kinase
  • MAPK3
  • Mapk3
  • mapk3
  • Tc00.1047053509475.10
  • Tb927.8.3550
  • CEK1
  • MPK3
Reactivity
Chicken, Cow (Bovine), Fruit Fly (Drosophila melanogaster), Human, Mouse (Murine), Rat (Rattus), Sheep (Ovine), Xenopus laevis
530
296
267
50
41
37
37
23
20
20
20
10
10
9
9
8
7
4
3
2
2
2
1
1
1
1
Host
Rabbit
408
127
19
6
1
Clonality
Polyclonal
Conjugate
This MAPK3 antibody is conjugated to PE-Atto 594
27
25
23
14
13
13
4
4
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
2
2
2
1
1
1
1
1
1
1
1
1
Application
Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blotting (WB)
456
210
206
107
83
67
60
50
39
35
15
13
8
4
3
2
1
1
1
Options
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Pubmed
3 references available

Available images

Immunofluorescence (fixed cells) (IF/ICC) image for anti-Mitogen-Activated Protein Kinase 3 (MAPK3) antibody (PE-Atto 594) (ABIN2486923)
Western Blotting (WB) image for anti-Mitogen-Activated Protein Kinase 3 (MAPK3) antibody (PE-Atto 594) (ABIN2486923)
Immunohistochemistry (IHC) image for anti-Mitogen-Activated Protein Kinase 3 (MAPK3) antibody (PE-Atto 594) (ABIN2486923)
Immunofluorescence (fixed cells) (IF/ICC) image for anti-Mitogen-Activated Protein Kinase 3 (MAPK3) antibody (PE-Atto 594) (ABIN2486923)
Immunohistochemistry (IHC) image for anti-Mitogen-Activated Protein Kinase 3 (MAPK3) antibody (PE-Atto 594) (ABIN2486923)
Immunofluorescence (fixed cells) (IF/ICC) image for anti-Mitogen-Activated Protein Kinase 3 (MAPK3) antibody (PE-Atto 594) (ABIN2486923)
Catalog No. ABIN2486923
$ 468.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
30.38187 ABIN2486924 ICC FACS IF IHC WB PerCP Rabbit Log in to see Polyclonal 3
30.38187 ABIN2486919 ICC FACS IF IHC WB APC Rabbit Log in to see Polyclonal 3
30.38187 ABIN2486609 ICC FACS IF IHC WB Atto 655 Rabbit Log in to see Polyclonal 3
30.38187 ABIN2486607 ICC FACS IF IHC WB Atto 594 Rabbit Log in to see Polyclonal 3
30.38187 ABIN2486611 ICC FACS IF IHC WB Atto 700 Rabbit Log in to see Polyclonal 3
30.38187 ABIN2486920 ICC FACS IF IHC WB Biotin Rabbit Log in to see Polyclonal 3
30.38187 ABIN2486606 ICC FACS IF IHC WB Atto 565 Rabbit Log in to see Polyclonal 3
30.38187 ABIN2486605 ICC FACS IF IHC WB Atto 488 Rabbit Log in to see Polyclonal 3
30.38187 ABIN2486610 ICC FACS IF IHC WB Atto 680 Rabbit Log in to see Polyclonal 3
30.38187 ABIN2486921 ICC FACS IF IHC WB FITC Rabbit Log in to see Polyclonal 3
30.38187 ABIN2486922 ICC FACS IF IHC WB HRP Rabbit Log in to see Polyclonal 3
30.38187 ABIN2486926 ICC FACS IF IHC WB Streptavidin Rabbit Log in to see Polyclonal 3
30.38187 ABIN2486604 ICC FACS IF IHC WB Atto 390 Rabbit Log in to see Polyclonal 3
30.38187 ABIN2486608 ICC FACS IF IHC WB Atto 633 Rabbit Log in to see Polyclonal 3
30.38187 ABIN2486918 ICC FACS IF IHC WB Alkaline Phosphatase (AP) Rabbit Log in to see Polyclonal 3
30.38187 ABIN2486925 ICC FACS IF IHC WB PE Rabbit Log in to see Polyclonal 3
19.88187 ABIN361833 ICC FACS IF IHC WB Rabbit Log in to see Polyclonal 9
1 ABIN5551325 IF IHC (fro) IHC (p) WB Rabbit IgG Log in to see Polyclonal 0

General

Antigen Mitogen-Activated Protein Kinase 3 (MAPK3) Antibodies
Reactivity Chicken, Cow (Bovine), Fruit Fly (Drosophila melanogaster), Human, Mouse (Murine), Rat (Rattus), Sheep (Ovine), Xenopus laevis
(530), (296), (267), (50), (41), (37), (37), (23), (20), (20), (20), (10), (10), (9), (9), (8), (7), (4), (3), (2), (2), (2), (1), (1), (1), (1)
Host Rabbit
(408), (127), (19), (6), (1)
Clonality
Polyclonal
Conjugate This MAPK3 antibody is conjugated to PE-Atto 594
(27), (25), (23), (14), (13), (13), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blotting (WB)
(456), (210), (206), (107), (83), (67), (60), (50), (39), (35), (15), (13), (8), (4), (3), (2), (1), (1), (1)
Pubmed 3 references available
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Product Details anti-MAPK3 Antibody

Target Details MAPK3 Application Details Handling References for anti-MAPK3 antibody (ABIN2486923) Images
Specificity Detects ~44kda (ERK1) and ~42 kDa (ERK2).
Purification Peptide Affinity Purified
Immunogen A 35 residue synthetic peptide, corresponding to Erk1 MAP kinase with the CGG spacer group added and the peptide coupled to KLH.
Plasmids, Primers & others

Target Details MAPK3

Product Details anti-MAPK3 Antibody Application Details Handling References for anti-MAPK3 antibody (ABIN2486923) Images back to top
Antigen
Alternative Name ERK1 (MAPK3 Antibody Abstract)
Background The extracellular signal-regulated kinases 1 and 2 (ERK1 and ERK2), also called p44 and p42 MAP kinases, are members of the Mitogen Activated Protein Kinase (MAPK) family of proteins found in all eukaryotes. Because the 44 kDa ERK1 and the 42 kDa ERK2 are highly homologous and both function in the same protein kinase cascade, the two proteins are often referred to collectively as ERK1/2 or p44/p42 MAP kinase (1). They are both located in the cytosol and mitochondria (2). While the role of cytosol ERK1/2 is well studied and involved in multiple cellular functions (2), the role of mitochondrial ERK1/2 remains poorly understood. Both ERK 1 and 2 are activated by MEK1 or MEK2, by dual phosphorylation of a threonine and tyrosine residue in the activation loop (TEY motif) (1, 3). Either phosphorylation alone can induce an electrophoretic mobility shift, but both are required for activation of the kinase. This dual phosphorylation is efficiently detected by phosphorylation state-specific antibody directed to the pTEpY motif. Once activated, MAP kinases phosphorylate a broad spectrum of substrates, including cytoskeletal proteins, translation regulators, transcription factors, and the Rsk family of protein kinases (4). ERK1/2 activation is generally thought to confer a survival advantage to cells (5), however there is increasing evidence that suggests that the activation of ERK1/2 also contributes to cell death under certain conditions (5). ERK1/2 also is activated in neuronal and renal epithelial cells upon exposure to oxidative stress and toxicants or deprivation of growth factors, and inhibition of the ERK pathway blocks apoptosis (5).
Gene ID 50689
NCBI Accession NP_059043
UniProt P21708
Pathways MAPK Signaling, RTK Signaling, Interferon-gamma Pathway, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, Response to Growth Hormone Stimulus, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Hepatitis C, Protein targeting to Nucleus, Toll-Like Receptors Cascades, Signaling Events mediated by VEGFR1 and VEGFR2, Signaling of Hepatocyte Growth Factor Receptor, VEGFR1 Specific Signals

Application Details

Product Details anti-MAPK3 Antibody Target Details MAPK3 Handling References for anti-MAPK3 antibody (ABIN2486923) Images back to top
Application Notes
  • WB (1:1000)
  • IHC (1:100)
  • ICC/IF (1:100)
  • FCM (1:100)
  • optimal dilutions for assays should be determined by the user.
Comment

A 1:1000 dilution of SPC-120 was sufficient for detection of ERK1/2 in 20 μg of HeLa cell lysate by ECL immunoblot analysis.
Restrictions For Research Use only

Handling

Product Details anti-MAPK3 Antibody Target Details MAPK3 Application Details References for anti-MAPK3 antibody (ABIN2486923) Images back to top
Format Liquid
Concentration 1 mg/mL
Buffer PBS pH 7.4, 50 % glycerol, 0.09 % sodium azide
Storage -20 °C

References for anti-MAPK3 antibody (ABIN2486923)

Product Details anti-MAPK3 Antibody Target Details MAPK3 Application Details Handling Images back to top
Background publications

Yoon, Seger: "The extracellular signal-regulated kinase: multiple substrates regulate diverse cellular functions." in: Growth factors (Chur, Switzerland), Vol. 24, Issue 1, pp. 21-44, 2006

Wolf: "Role of reactive oxygen species in angiotensin II-mediated renal growth, differentiation, and apoptosis." in: Antioxidants & redox signaling, Vol. 7, Issue 9-10, pp. 1337-45, 2005

Boulton, Gregory, Cobb: "Purification and properties of extracellular signal-regulated kinase 1, an insulin-stimulated microtubule-associated protein 2 kinase." in: Biochemistry, Vol. 30, Issue 1, pp. 278-86, 1991

Images

Product Details anti-MAPK3 Antibody Target Details MAPK3 Application Details Handling References for anti-MAPK3 antibody (ABIN2486923) back to top
Supplier Images
Immunofluorescence (fixed cells) (IF/ICC) image for anti-Mitogen-Activated Protein Kinase 3 (MAPK3) antibody (PE-Atto 594) (ABIN2486923) Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-Erk1/2 Polyclonal A...
Western Blotting (WB) image for anti-Mitogen-Activated Protein Kinase 3 (MAPK3) antibody (PE-Atto 594) (ABIN2486923) Western blot analysis of Human Cell line lysates showing detection of ERK1 protein us...
Immunohistochemistry (IHC) image for anti-Mitogen-Activated Protein Kinase 3 (MAPK3) antibody (PE-Atto 594) (ABIN2486923) Immunohistochemistry analysis using Rabbit Anti-ERK1 Polyclonal Antibody . Tissue: In...
Immunofluorescence (fixed cells) (IF/ICC) image for anti-Mitogen-Activated Protein Kinase 3 (MAPK3) antibody (PE-Atto 594) (ABIN2486923) Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-ERK1 Polyclonal Ant...
Immunohistochemistry (IHC) image for anti-Mitogen-Activated Protein Kinase 3 (MAPK3) antibody (PE-Atto 594) (ABIN2486923) Immunohistochemistry analysis using Rabbit Anti-ERK1 Polyclonal Antibody . Tissue: ba...
Immunofluorescence (fixed cells) (IF/ICC) image for anti-Mitogen-Activated Protein Kinase 3 (MAPK3) antibody (PE-Atto 594) (ABIN2486923) Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-Erk1/2 Polyclonal A...