anti-Sheep (Ovine) MAPK3 antibody for Immunofluorescence
Recommended MAPK3 Antibody (supplied by: Log in to see )
- Antigen
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Mitogen-Activated Protein Kinase 3 (MAPK3) Antibodies
show synonyms for this antigen- ERK-1
- ERK1
- ERT2
- HS44KDAP
- HUMKER1A
- P44ERK1
- P44MAPK
- PRKM3
- p44-ERK1
- p44-MAPK
- Erk-1
- Erk1
- Ert2
- Esrk1
- Mnk1
- Mtap2k
- Prkm3
- p44
- p44erk1
- p44mapk
- fi06b09
- wu:fi06b09
- zERK1
- Tb08.10J17.940
- erk6
- etID309866.18
- mapk12
- sapk3
- wu:fa05c12
- zgc:101695
- MAPK1
- MNK1
- ATMAPK3
- ATMPK3
- T6D9.4
- mitogen-activated protein kinase 3
- mitogen-activated protein kinase 3
- mitogen-activated protein kinase 12a
- mitogen activated protein kinase 3
- mitogen-activated serine/threonine-protein kinase
- MAPK3
- Mapk3
- mapk3
- Tc00.1047053509475.10
- Tb927.8.3550
- mapk12a
- CEK1
- MPK3
- Reactivity
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Chicken, Cow (Bovine), Fruit Fly (Drosophila melanogaster), Human, Mouse (Murine), Rat (Rattus), Sheep (Ovine), Xenopus laevis
Alternatives - Host
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Rabbit
Alternatives - Clonality
- Conjugate
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This MAPK3 antibody is un-conjugated
Alternatives - Application
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Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blotting (WB)
Alternatives - Options
- Supplier
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- Supplier Product No.
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| Relevance Score | ABIN | Application | Conjugate | Host | Isotype | Epitope | Supplier | Clonality | References | Details |
|---|---|---|---|---|---|---|---|---|---|---|
| 1 | ABIN2486608 | ICC FACS IF IHC WB | Atto 633 | Rabbit | Log in to see | Polyclonal | 3 | |||
| 1 | ABIN2486610 | ICC FACS IF IHC WB | Atto 680 | Rabbit | Log in to see | Polyclonal | 3 | |||
| 1 | ABIN2486919 | ICC FACS IF IHC WB | APC | Rabbit | Log in to see | Polyclonal | 3 | |||
| 1 | ABIN2486920 | ICC FACS IF IHC WB | Biotin | Rabbit | Log in to see | Polyclonal | 3 | |||
| 1 | ABIN2486921 | ICC FACS IF IHC WB | FITC | Rabbit | Log in to see | Polyclonal | 3 | |||
| 1 | ABIN2486923 | ICC FACS IF IHC WB | PE-Atto 594 | Rabbit | Log in to see | Polyclonal | 3 | |||
| 1 | ABIN2486926 | ICC FACS IF IHC WB | Streptavidin | Rabbit | Log in to see | Polyclonal | 3 | |||
| 1 | ABIN2486922 | ICC FACS IF IHC WB | HRP | Rabbit | Log in to see | Polyclonal | 3 | |||
| 1 | ABIN2486604 | ICC FACS IF IHC WB | Atto 390 | Rabbit | Log in to see | Polyclonal | 3 | |||
| 1 | ABIN2486606 | ICC FACS IF IHC WB | Atto 565 | Rabbit | Log in to see | Polyclonal | 3 | |||
| 1 | ABIN2486605 | ICC FACS IF IHC WB | Atto 488 | Rabbit | Log in to see | Polyclonal | 3 | |||
| 1 | ABIN2486925 | ICC FACS IF IHC WB | PE | Rabbit | Log in to see | Polyclonal | 3 | |||
| 1 | ABIN2486607 | ICC FACS IF IHC WB | Atto 594 | Rabbit | Log in to see | Polyclonal | 3 | |||
| 1 | ABIN2486609 | ICC FACS IF IHC WB | Atto 655 | Rabbit | Log in to see | Polyclonal | 3 | |||
| 1 | ABIN2486918 | ICC FACS IF IHC WB | Alkaline Phosphatase (AP) | Rabbit | Log in to see | Polyclonal | 3 | |||
| 1 | ABIN2486924 | ICC FACS IF IHC WB | PerCP | Rabbit | Log in to see | Polyclonal | 3 | |||
| 1 | ABIN2486611 | ICC FACS IF IHC WB | Atto 700 | Rabbit | Log in to see | Polyclonal | 3 | |||
| 1 | ABIN5551325 | IF IHC (fro) IHC (p) WB | Rabbit | IgG | Log in to see | Polyclonal | 0 |
General |
|
|---|---|
| Antigen | Mitogen-Activated Protein Kinase 3 (MAPK3) Antibodies |
| Reactivity | Chicken, Cow (Bovine), Fruit Fly (Drosophila melanogaster), Human, Mouse (Murine), Rat (Rattus), Sheep (Ovine), Xenopus laevis Alternatives |
| Host | Rabbit Alternatives |
| Clonality | |
| Conjugate | This MAPK3 antibody is un-conjugated Alternatives |
| Application |
Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blotting (WB)
Alternatives
|
|
9 references available |
| Supplier | Log in to see |
Product Details anti-MAPK3 AntibodyTarget Details MAPK3 Application Details Handling References for anti-MAPK3 antibody (ABIN361833) Images |
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| Specificity | Detects ~44kda (ERK1) and ~42 kDa (ERK2). |
| Purification | Peptide Affinity Purified |
| Immunogen | A 35 residue synthetic peptide, corresponding to Erk1 MAP kinase with the CGG spacer group added and the peptide coupled to KLH. |
| Plasmids, Primers & others | |
Target Details MAPK3Product Details anti-MAPK3 Antibody Application Details Handling References for anti-MAPK3 antibody (ABIN361833) Images back to top |
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| Antigen | |
| Alternative Name | ERK1 (MAPK3 Antibody Abstract) |
| Background | The extracellular signal-regulated kinases 1 and 2 (ERK1 and ERK2), also called p44 and p42 MAP kinases, are members of the Mitogen Activated Protein Kinase (MAPK) family of proteins found in all eukaryotes. Because the 44 kDa ERK1 and the 42 kDa ERK2 are highly homologous and both function in the same protein kinase cascade, the two proteins are often referred to collectively as ERK1/2 or p44/p42 MAP kinase (1). They are both located in the cytosol and mitochondria (2). While the role of cytosol ERK1/2 is well studied and involved in multiple cellular functions (2), the role of mitochondrial ERK1/2 remains poorly understood. Both ERK 1 and 2 are activated by MEK1 or MEK2, by dual phosphorylation of a threonine and tyrosine residue in the activation loop (TEY motif) (1, 3). Either phosphorylation alone can induce an electrophoretic mobility shift, but both are required for activation of the kinase. This dual phosphorylation is efficiently detected by phosphorylation state-specific antibody directed to the pTEpY motif. Once activated, MAP kinases phosphorylate a broad spectrum of substrates, including cytoskeletal proteins, translation regulators, transcription factors, and the Rsk family of protein kinases (4). ERK1/2 activation is generally thought to confer a survival advantage to cells (5), however there is increasing evidence that suggests that the activation of ERK1/2 also contributes to cell death under certain conditions (5). ERK1/2 also is activated in neuronal and renal epithelial cells upon exposure to oxidative stress and toxicants or deprivation of growth factors, and inhibition of the ERK pathway blocks apoptosis (5). |
| Gene ID | 50689 |
| NCBI Accession | NP_059043 |
| UniProt | P21708 |
| Pathways | MAPK Signaling, RTK Signaling, Interferon-gamma Pathway, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, Response to Growth Hormone Stimulus, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Hepatitis C, Protein targeting to Nucleus, Toll-Like Receptors Cascades, Signaling Events mediated by VEGFR1 and VEGFR2, Signaling of Hepatocyte Growth Factor Receptor, VEGFR1 Specific Signals |
Application DetailsProduct Details anti-MAPK3 Antibody Target Details MAPK3 Handling References for anti-MAPK3 antibody (ABIN361833) Images back to top |
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| Application Notes |
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| Comment |
A 1:1000 dilution of SPC-120 was sufficient for detection of ERK1/2 in 20 μg of HeLa cell lysate by ECL immunoblot analysis. |
| Restrictions | For Research Use only |
HandlingProduct Details anti-MAPK3 Antibody Target Details MAPK3 Application Details References for anti-MAPK3 antibody (ABIN361833) Images back to top |
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| Format | Liquid |
| Concentration | 1 mg/mL |
| Buffer | PBS pH 7.4, 50 % glycerol, 0.09 % sodium azide |
| Storage | -20 °C |
References for anti-MAPK3 antibody (ABIN361833)Product Details anti-MAPK3 Antibody Target Details MAPK3 Application Details Handling Images back to top |
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| Product cited in: |
Revuelta-López, Cal, Herraiz-Martínez, de Gonzalo-Calvo, Nasarre, Roura, Gálvez-Montón, Bayes-Genis, Badimon, Hove-Madsen, Llorente-Cortés: "Hypoxia-driven sarcoplasmic/endoplasmic reticulum calcium ATPase 2 (SERCA2) downregulation depends on low-density lipoprotein receptor-related protein 1 (LRP1)-signalling in cardiomyocytes." in: Journal of molecular and cellular cardiology, Vol. 85, pp. 25-36, 2015 Thon, Hosoi, Yoshii, Ozawa: "Leptin induced GRP78 expression through the PI3K-mTOR pathway in neuronal cells." in: Scientific reports, Vol. 4, pp. 7096, 2014 Bhat, Gomis, Potter, Tikoo: "Role of Hsp90 in CpG ODN mediated immunostimulation in avian macrophages." in: Molecular immunology, Vol. 47, Issue 6, pp. 1337-46, 2010 Enikanolaiye, Larivière, Troy, Arabzadeh, Atasoy, Turksen: "Involucrin-claudin-6 tail deletion mutant (CDelta206) transgenic mice: a model of delayed epidermal permeability barrier formation and repair." in: Disease models & mechanisms, Vol. 3, Issue 3-4, pp. 167-80, 2010 |
| Background publications |
Chuderland, Marmor, Shainskaya, Seger: "Calcium-mediated interactions regulate the subcellular localization of extracellular signal-regulated kinases." in: The Journal of biological chemistry, Vol. 283, Issue 17, pp. 11176-88, 2008 Yoon, Seger: "The extracellular signal-regulated kinase: multiple substrates regulate diverse cellular functions." in: Growth factors (Chur, Switzerland), Vol. 24, Issue 1, pp. 21-44, 2006 Zhuang, Schnellmann: "A death-promoting role for extracellular signal-regulated kinase." in: The Journal of pharmacology and experimental therapeutics, Vol. 319, Issue 3, pp. 991-7, 2006 Wolf: "Role of reactive oxygen species in angiotensin II-mediated renal growth, differentiation, and apoptosis." in: Antioxidants & redox signaling, Vol. 7, Issue 9-10, pp. 1337-45, 2005 Boulton, Gregory, Cobb: "Purification and properties of extracellular signal-regulated kinase 1, an insulin-stimulated microtubule-associated protein 2 kinase." in: Biochemistry, Vol. 30, Issue 1, pp. 278-86, 1991 |
ImagesProduct Details anti-MAPK3 Antibody Target Details MAPK3 Application Details Handling References for anti-MAPK3 antibody (ABIN361833) back to top |
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| Supplier Images |
Erk1 2 Western Blotting 1 in 1000 human cell line mix 10ug.
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