anti-Monkey RAF1 antibody for Immunoprecipitation

Recommended RAF1 Antibody (supplied by: Log in to see )

Antigen
V-Raf-1 Murine Leukemia Viral Oncogene Homolog 1 (RAF1) Antibodies
  • CRAF
  • NS5
  • Raf-1
  • c-Raf
  • c-raf
  • craf
  • raf1
  • RAF1
  • Afr-1
  • Afr1
  • Raf
  • mKIAA0854
  • 6430402F14Rik
  • AA990557
  • BB129353
  • Craf1
  • D830050J10Rik
  • v-Raf
  • raf
  • cRaf
  • Raf-1 proto-oncogene, serine/threonine kinase
  • Raf-1 proto-oncogene, serine/threonine kinase a
  • zinc fingers and homeoboxes 2
  • v-raf-leukemia viral oncogene 1
  • Raf-1 proto-oncogene, serine/threonine kinase S homeolog
  • RAF1
  • Raf1
  • raf1a
  • raf1
  • Zhx2
  • raf1.S
Epitope
AA 657-668
85
77
49
49
48
39
34
34
34
32
32
22
21
21
20
19
16
15
15
15
14
14
10
10
9
8
8
7
6
5
5
5
4
4
4
4
4
4
4
3
3
3
3
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Bat, Cow (Bovine), Dog (Canine), Hamster, Horse (Equine), Human, Monkey, Mouse (Murine), Rabbit, Rat (Rattus)
917
481
452
24
23
19
16
15
8
7
7
5
4
4
2
1
1
Host
Sheep
866
62
12
5
1
Clonality
Polyclonal
Conjugate
This RAF1 antibody is un-conjugated
46
46
45
30
30
29
14
14
12
12
12
12
12
12
12
12
12
2
2
2
2
2
2
2
2
2
1
1
Application
Immunoprecipitation (IP), Western Blotting (WB)
586
439
270
182
156
124
65
50
31
18
15
10
2
2
2
1
Supplier
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Catalog No. ABIN295906
$ 625.17
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN233706 IP WB Rabbit Pro302 Log in to see Polyclonal 0

General

Antigen V-Raf-1 Murine Leukemia Viral Oncogene Homolog 1 (RAF1) Antibodies
Epitope AA 657-668
(85), (77), (49), (49), (48), (39), (34), (34), (34), (32), (32), (22), (21), (21), (20), (19), (16), (15), (15), (15), (14), (14), (10), (10), (9), (8), (8), (7), (6), (5), (5), (5), (4), (4), (4), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Bat, Cow (Bovine), Dog (Canine), Hamster, Horse (Equine), Human, Monkey, Mouse (Murine), Rabbit, Rat (Rattus)
(917), (481), (452), (24), (23), (19), (16), (15), (8), (7), (7), (5), (4), (4), (2), (1), (1)
Host Sheep
(866), (62), (12), (5), (1)
Clonality
Polyclonal
Conjugate This RAF1 antibody is un-conjugated
(46), (46), (45), (30), (30), (29), (14), (14), (12), (12), (12), (12), (12), (12), (12), (12), (12), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1)
Application Immunoprecipitation (IP), Western Blotting (WB)
(586), (439), (270), (182), (156), (124), (65), (50), (31), (18), (15), (10), (2), (2), (2), (1)
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Product Details anti-RAF1 Antibody

Target Details RAF1 Application Details Handling Images
Specificity Recognizes Raf-1, Mr ~70kD. Species cross-reactivity: Human. Predicted to cross-react with mouse and rat based on sequence homology.
Predicted Reactivity Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Dog, Bat, Bovine, Horse, Rabbit (100%).
Purification Protein A purified
Immunogen 12 residue synthetic C terminal peptide (CTLTTSPRLPVF) corresponding to residues 637-648 of human Raf-1. Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Dog, Bat, Bovine, Horse, Rabbit (100%).

Type of Immunogen: Synthetic peptide
Isotype IgG
Plasmids, Primers & others

Target Details RAF1

Product Details anti-RAF1 Antibody Application Details Handling Images back to top
Antigen
Alternative Name RAF1 / RAF (RAF1 Antibody Abstract)
Background Name/Gene ID: RAF1
Subfamily: Raf
Family: Protein Kinase

Synonyms: RAF1, C-Raf, C-raf-1, CRAF, NS5, Raf-1, Proto-oncogene c-RAF, Oncogene RAF1, RAF, Raf1 kinase
Gene ID 5894
UniProt P04049
Pathways MAPK Signaling, RTK Signaling, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, cAMP Metabolic Process, Stem Cell Maintenance, Hepatitis C, Autophagy, Signaling of Hepatocyte Growth Factor Receptor, VEGF Signaling

Application Details

Product Details anti-RAF1 Antibody Target Details RAF1 Handling Images back to top
Application Notes Approved: IP, WB

Usage: Suitable for use in Western Blot, Immunoprecipitation. Western Blot Analysis: 2 μg/mL detects Raf-1 in RIPA lysates from 3T3/A31 cell lysates. 3T3/A31 cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with anti-Raf-1 (2 μg/mL). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. Included Positive Antigen Control: 3T3/A31 cell lysate. Add 2.5 μL of 2-mercaptoethanol/100 μL of lysate and boil for 5 minutes to reduce the preparation. Load 20 μg of reduced lysate per lane for minigels. MAPK Activation and MBP Phosphorylation: 4 μg immunoprecipitates Raf-1. The immunocomplex was then used in a coupled phosphorylation assay to activate MEK1, which in turn activates MAP Kinase 2 that phosphorylates myelin basic protein in vitro.
Comment

Target Species of Antibody: Human

Restrictions For Research Use only

Handling

Product Details anti-RAF1 Antibody Target Details RAF1 Application Details Images back to top
Format Liquid
Concentration Lot specific
Buffer 0.1 M Tris-glycine,  pH 7.4, 0.15 M sodium chloride, 0.05 % sodium azide, 30 % glycerol.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeat freeze-thaw cycles.
Storage 4 °C,-20 °C
Storage Comment Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.