anti-Mouse (Murine) SIRT1 antibody for ELISA

Recommended SIRT1 Antibody (supplied by: Log in to see )

Antigen
Sirtuin 1 (SIRT1) Antibodies
  • sirtuin 1
  • AA673258
  • SIR2L1
  • Sir2
  • Sir2a
  • Sir2alpha
  • sir2l1
  • sirtuin
  • SIR2
  • sirtuin 1
  • sirtuin 1 L homeolog
  • SIRT1
  • SRT1
  • Sirt1
  • sirt1.L
Epitope
Internal Region
25
24
21
17
15
15
12
11
10
6
6
5
4
3
3
3
3
3
3
3
3
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Mouse (Murine)
301
139
69
21
14
11
7
7
7
6
5
4
4
2
2
2
2
1
Host
Goat
201
86
27
6
3
Clonality
Polyclonal
Conjugate
This SIRT1 antibody is un-conjugated
16
14
9
7
7
6
6
4
4
4
4
4
3
3
3
3
3
3
3
3
3
3
3
2
2
2
2
1
Method Type
Peptide ELISA
Application
ELISA, Western Blotting (WB)
254
138
84
66
60
51
42
40
33
7
6
4
4
2
2
2
2
2
1
1
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Catalog No. ABIN185702
$ 358.02
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN4353901 ELISA FACS ICC IF IHC IHC (p) WB Mouse IgG1 Log in to see 1F3 1
1 ABIN1450758 IHC ELISA WB Rabbit IgG AA 13-62 Log in to see Polyclonal 3
1 ABIN3186962 ELISA IHC WB Rabbit IgG Tyr631 Log in to see Polyclonal 0
1 ABIN2460449 ELISA WB Rabbit Log in to see Polyclonal 0
1 ABIN2460448 ELISA WB Rabbit Log in to see Polyclonal 0
1 ABIN1457379 IHC ELISA WB Rabbit IgG AA 41-90 Log in to see Polyclonal 0
1 ABIN1031567 IF ELISA WB Chicken N-Term Log in to see Polyclonal 0
1 ABIN203406 ELISA Rabbit AA 646-660 Log in to see Polyclonal 0
1 ABIN2559927 ELISA WB Goat IgG Internal Region Log in to see Polyclonal 0
1 ABIN3182378 ELISA WB Rabbit IgG pSer47 Log in to see Polyclonal 0
1 ABIN5688245 ELISA WB Goat Internal Region Log in to see Polyclonal 0
1 ABIN574544 IF ELISA WB Chicken N-Term Log in to see Polyclonal 0
1 ABIN2885319 IHC ELISA WB Rabbit IgG AA 41-90 Log in to see Polyclonal 0
1 ABIN2747513 ELISA IHC IP WB Rabbit IgG AA 25-75 Log in to see Polyclonal 0
1 ABIN2360958 ELISA WB Alkaline Phosphatase (AP) Goat Log in to see Polyclonal 0
1 ABIN2360960 ELISA WB Biotin Goat Log in to see Polyclonal 0
1 ABIN2360961 ELISA WB FITC Goat Log in to see Polyclonal 0
1 ABIN2360959 ELISA WB APC Goat Log in to see Polyclonal 0
1 ABIN2360963 ELISA WB PE Goat Log in to see Polyclonal 0
1 ABIN2559933 IHC ELISA WB Goat IgG AA 577-590 Log in to see Polyclonal 0

General

Antigen Sirtuin 1 (SIRT1) Antibodies
Epitope Internal Region
(25), (24), (21), (17), (15), (15), (12), (11), (10), (6), (6), (5), (4), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Mouse (Murine)
(301), (139), (69), (21), (14), (11), (7), (7), (7), (6), (5), (4), (4), (2), (2), (2), (2), (1)
Host Goat
(201), (86), (27), (6), (3)
Clonality
Polyclonal
Conjugate This SIRT1 antibody is un-conjugated
(16), (14), (9), (7), (7), (6), (6), (4), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (1)
Method Type Peptide ELISA
Application ELISA, Western Blotting (WB)
(254), (138), (84), (66), (60), (51), (42), (40), (33), (7), (6), (4), (4), (2), (2), (2), (2), (2), (1), (1)
Pubmed 2 references available
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Product Details anti-SIRT1 Antibody

Target Details SIRT1 Application Details Handling References for anti-SIRT1 antibody (ABIN185702) Images
Blocking Peptide Blocking peptide for this product available: ABIN368993
Cross-Reactivity Human, Rat (Rattus), Dog (Canine)
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen Peptide with sequence C-EITEKPPRTQKE, from the internal region of the protein sequence according to NP_036370.2.

Target Details SIRT1

Product Details anti-SIRT1 Antibody Application Details Handling References for anti-SIRT1 antibody (ABIN185702) Images back to top
Antigen
Alternative Name SIRT1 (SIRT1 Antibody Abstract)
Background Synonyms: SIRT1, sirtuin (silent mating type information regulation 2 homolog) 1 (S. cerevisiae), RP11-57G10.3, SIR2L1, OTTHUMP00000060745, SIR2alpha, sir2-like 1, sirtuin (silent mating type information regulation 2, S. cerevisiae, homolog) 1, sirtuin 1, sirtuin type 1
Gene ID 23411, 93759, 309757
NCBI Accession NP_036370
Pathways MAPK Signaling, Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Carbohydrate Homeostasis, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process, Positive Regulation of Response to DNA Damage Stimulus, Negative Regulation of intrinsic apoptotic Signaling

Application Details

Product Details anti-SIRT1 Antibody Target Details SIRT1 Handling References for anti-SIRT1 antibody (ABIN185702) Images back to top
Application Notes ELISA: antibody detection limit dilution 1:64000.
Western blot: Approx 120 kDa band observed in Mouse fetal Brain lysates (calculated MW of 80.3 kDa according to Muose NP_062786.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Cheng et al, Proc Natl Acad Sci U S A. 2003 Sep 16,100(19):10794-9., PMID: 12960381). Recommended concentration: 1-3 µg/mL.
Restrictions For Research Use only

Handling

Product Details anti-SIRT1 Antibody Target Details SIRT1 Application Details References for anti-SIRT1 antibody (ABIN185702) Images back to top
Format Liquid
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.

References for anti-SIRT1 antibody (ABIN185702)

Product Details anti-SIRT1 Antibody Target Details SIRT1 Application Details Handling Images back to top
Background publications

Pruitt, Zinn, Ohm, McGarvey, Kang, Watkins, Herman, Baylin: "Inhibition of SIRT1 reactivates silenced cancer genes without loss of promoter DNA hypermethylation." in: PLoS genetics, Vol. 2, Issue 3, pp. e40, 2006

Chen, Steele, Lindquist, Guarente: "Increase in activity during calorie restriction requires Sirt1." in: Science (New York, N.Y.), Vol. 310, Issue 5754, pp. 1641, 2005

Images

Product Details anti-SIRT1 Antibody Target Details SIRT1 Application Details Handling References for anti-SIRT1 antibody (ABIN185702) back to top
Supplier Images
 image for anti-Sirtuin 1 (SIRT1) (Internal Region) antibody (ABIN185702) ABIN185702 (0.1µg/ml) staining of HeLa cell lysate (35µg protein in RIPA buffer). Pri...