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|Antigen||Yes-Associated Protein 1 (YAP1) Antibodies|
|Reactivity||Human, Mouse (Murine) Alternatives|
|Conjugate||This YAP1 antibody is un-conjugated Alternatives|
Chromatin Immunoprecipitation (ChIP), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Simple Western (SimWes), Western Blotting (WB)
|16 references available|
|Supplier||Log in to see|
Product Details anti-YAP1 AntibodyTarget Details YAP1 Application Details Handling References for anti-YAP1 antibody (ABIN258559) Images
|Purification||Immunogen affinity purified|
|Immunogen||Recombinant human YAP1 protein expressed in bacteria. [UniProt# P46937]|
Target Details YAP1Product Details anti-YAP1 Antibody Application Details Handling References for anti-YAP1 antibody (ABIN258559) Images back to top
|Alternative Name||YAP1 (YAP1 Antibody Abstract)|
|Background||Gene Symbol: YAP1|
|Molecular Weight||Theoretical MW: 65 kDa|
|Pathways||MAPK Signaling, Stem Cell Maintenance, Regulation of Lipid Metabolism by PPARalpha|
Application DetailsProduct Details anti-YAP1 Antibody Target Details YAP1 Handling References for anti-YAP1 antibody (ABIN258559) Images back to top
|Application Notes||Western Blot 1:1000, Simple Western 1:12.5, Chromatin Immunoprecipitation, Immunohistochemistry 1:50-1:200, Immunocytochemistry/Immunofluorescence 1:500, Immunoprecipitation 2-10 μg, Immunohistochemistry-Paraffin 1:50-1:200This YAP1 antibody is useful for Immunocytochemistry/Immunofluorescence, Western blot, Immunoprecipitation, Chromatin Immunoprecipitation and Immunohistochemistry paraffin-embedded sections. The Western blot was performed with transfected HEK 293 lysates, where a band was detected approx. 65 kDa. Its use in ChIP application has been reported in PMID 20516196. In Simple Western only 10 - 15 μL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.|
The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.
|Restrictions||For Research Use only|
HandlingProduct Details anti-YAP1 Antibody Target Details YAP1 Application Details References for anti-YAP1 antibody (ABIN258559) Images back to top
PBS, 15 % glycerol
Buffer contains: 0.02 % Sodium Azide
|Precaution of Use||This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid freeze-thaw cycles|
|Storage||-80 °C,-20 °C|
|Storage Comment||Aliquot and store at -20°C or -80°C. Avoid freeze-thaw cycles.|
References for anti-YAP1 antibody (ABIN258559)Product Details anti-YAP1 Antibody Target Details YAP1 Application Details Handling Images back to top
|Product cited in:||
Lin, Yao, Zhang, Jiang, Croll, Thompson-Peer, Chuang: "YAP is essential for mechanical force production and epithelial cell proliferation during lung branching morphogenesis." in: eLife, Vol. 6, 2017 Method employed by authors: Immunofluorescence (fixed cells) (IF/ICC) (Sample species: Mouse (Murine)).
Plubell, Wilmarth, Zhao, Fenton, Minnier, Reddy, Klimek, Yang, David, Pamir: "Extended Multiplexing of Tandem Mass Tags (TMT) Labeling Reveals Age and High Fat Diet Specific Proteome Changes in Mouse Epididymal Adipose Tissue." in: Molecular & cellular proteomics : MCP, Vol. 16, Issue 5, pp. 873-890, 2017
Morikawa, Heallen, Leach, Xiao, Martin: "Dystrophin-glycoprotein complex sequesters Yap to inhibit cardiomyocyte proliferation." in: Nature, Vol. 547, Issue 7662, pp. 227-231, 2017 Method employed by authors: Immunohistochemistry (Frozen Sections) (IHC (fro)) (Sample species: Mouse (Murine)).
Sarkar, Bristow, Dey, Rai, Perets, Ramirez-Cardenas, Malasi, Huang-Hobbs, Haemmerle, Wu, McGuire, Protopopov, Jiang, Liu, Hirsch, Chang, Lazar, Sood, Drapkin, DePinho, Draetta, Chin: "PRKCI promotes immune suppression in ovarian cancer." in: Genes & development, Vol. 31, Issue 11, pp. 1109-1121, 2017 (Sample species: Human).
Rizvi, Yamada, Hirsova, Bronk, Werneburg, Krishnan, Salim, Zhang, Trushina, Truty, Gores: "A Hippo and Fibroblast Growth Factor Receptor Autocrine Pathway in Cholangiocarcinoma." in: The Journal of biological chemistry, Vol. 291, Issue 15, pp. 8031-47, 2016 (Sample species: Human). Further details: Immunohistochemistry (Frozen Sections)
Tao, Kahr, Morikawa, Zhang, Rahmani, Heallen, Li, Sun, Olson, Amendt, Martin: "Pitx2 promotes heart repair by activating the antioxidant response after cardiac injury." in: Nature, Vol. 534, Issue 7605, pp. 119-23, 2016 (Sample species: Mouse (Murine)). Further details: Western Blotting,Chromatin Immunoprecipitation
Karystinou, Roelofs, Neve, Cantatore, Wackerhage, De Bari: "Yes-associated protein (YAP) is a negative regulator of chondrogenesis in mesenchymal stem cells." in: Arthritis research & therapy, Vol. 17, pp. 147, 2015 (Sample species: Mouse (Murine)). Further details: Immunohistochemistry
Chen, Zhang, Xie, Wang, Cai, Choi, David, Huang, Yabuta, Nojima, Anders, Pan: "Homeostatic control of Hippo signaling activity revealed by an endogenous activating mutation in YAP." in: Genes & development, Vol. 29, Issue 12, pp. 1285-97, 2015 (Sample species: Mouse (Murine)). Further details: Western Blotting
Cai, Maitra, Anders, Taketo, Pan: "β-Catenin destruction complex-independent regulation of Hippo-YAP signaling by APC in intestinal tumorigenesis." in: Genes & development, Vol. 29, Issue 14, pp. 1493-506, 2015 Further details: Western Blotting
Chen, Lin, Tang: "Matrix-Stiffness-Regulated Inverse Expression of Krüppel-Like Factor 5 and Krüppel-Like Factor 4 in the Pathogenesis of Renal Fibrosis." in: The American journal of pathology, Vol. 185, Issue 9, pp. 2468-81, 2015 (Sample species: Mouse (Murine)). Further details: Immunocytochemistry,Immunohistochemistry (Paraffin-embedded Sections),Immunofluorescence
Brusgard, Choe, Chumsri, Renoud, MacKerell, Sudol, Passaniti: "RUNX2 and TAZ-dependent signaling pathways regulate soluble E-Cadherin levels and tumorsphere formation in breast cancer cells." in: Oncotarget, Vol. 6, Issue 29, pp. 28132-50, 2015 Further details: Western Blotting
Kapoor, Yao, Ying, Hua, Liewen, Wang, Zhong, Wu, Sadanandam, Hu, Chang, Chu, Al-Khalil, Jiang, Xia, Fletcher-Sananikone, Lim, Horwitz, Viale, Pettazzoni, Sanchez, Wang, Protopopov, Zhang, Heffernan et al.: "Yap1 activation enables bypass of oncogenic Kras addiction in pancreatic cancer. ..." in: Cell, Vol. 158, Issue 1, pp. 185-97, 2014 Further details: Western Blotting,Immunohistochemistry (Paraffin-embedded Sections),Immunoprecipitation
Tsujiura, Mazack, Sudol, Kaspar, Nash, Carey, Gogoi: "Yes-associated protein (YAP) modulates oncogenic features and radiation sensitivity in endometrial cancer." in: PLoS ONE, Vol. 9, Issue 6, pp. e100974, 2014 (Sample species: Human). Further details: Immunocytochemistry,Western Blotting,Immunohistochemistry (Paraffin-embedded Sections),Immunofluorescence
Miao, Pan, Lei, Wen, Li, Liu, Qiu, Lin, Chen, Chen: "Knockdown of GPC3 inhibits the proliferation of Huh7 hepatocellular carcinoma cells through down-regulation of YAP." in: Journal of cellular biochemistry, Vol. 114, Issue 3, pp. 625-31, 2013 (Sample species: Human). Further details: Western Blotting
Kim, Kim, Song, Kim, Cho, Moon, Ro, Seo, Ryu, Myung, Jho: "Deubiquitinase YOD1 potentiates YAP/TAZ activities through enhancing ITCH stability." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 114, Issue 18, pp. 4691-4696, 1970 Method employed by authors: Immunohistochemistry (IHC) (Sample species: Human).
Zheng, Liu, Wang, Lei, Pulgar Prieto, Pan: "Homeostatic Control of Hpo/MST Kinase Activity through Autophosphorylation-Dependent Recruitment of the STRIPAK PP2A Phosphatase Complex." in: Cell reports, Vol. 21, Issue 12, pp. 3612-3623, 1970 Method employed by authors: Western Blotting (WB) (Sample species: Fruit Fly (Drosophila melanogaster)).
ImagesProduct Details anti-YAP1 Antibody Target Details YAP1 Application Details Handling References for anti-YAP1 antibody (ABIN258559) back to top