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|Antigen||rho-Associated, Coiled-Coil Containing Protein Kinase 2 (ROCK2) Antibodies|
|Epitope||AA 567-718 Alternatives|
|Reactivity||Dog (Canine), Human, Mouse (Murine), Rat (Rattus) Alternatives|
|Conjugate||This ROCK2 antibody is un-conjugated Alternatives|
Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
|5 references available|
|Supplier||Log in to see|
Product Details anti-ROCK2 AntibodyTarget Details ROCK2 Application Details Handling References for anti-ROCK2 antibody (ABIN968071) Images
|Cross-Reactivity||Mouse (Murine), Dog (Canine), Human|
1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
|Purification||The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.|
|Immunogen||Rat ROKalpha aa. 567-718|
Target Details ROCK2Product Details anti-ROCK2 Antibody Application Details Handling References for anti-ROCK2 antibody (ABIN968071) Images back to top
|Alternative Name||ROCK-II (ROCK2 Antibody Abstract)|
Activity of the GTP-binding proteins is regulated by GAPs, that accelerate binding, and GTPases, that enhance the rate of GTP hydrolysis (Ras, Rho, cdc42Hs, and Rac). The isoforms of Rho, a small GTP-binding protein, regulate cellular processes such as the formation of the stress fibers, lamelipodia, and filopodia. ROKalpha (RhoA-binding kinase) is a Ser/Thr protein kinase that interects with the GTP-binding form of RhoA. Like RhoA, ROKalpha localizes at the cellular margins and colocalizes with acti filaments. The ROKalpha gene encodes a protein of 1302 amino acids with homology to the human myotonic kinase. Although ROKalpha binds to GTP-RhoA, it is not yet clear whether this interaction induces the kinase activity of ROKalpha.
|Molecular Weight||180 kDa|
|Pathways||Microtubule Dynamics, WNT Signaling, Tube Formation|
Application DetailsProduct Details anti-ROCK2 Antibody Target Details ROCK2 Handling References for anti-ROCK2 antibody (ABIN968071) Images back to top
|Restrictions||For Research Use only|
HandlingProduct Details anti-ROCK2 Antibody Target Details ROCK2 Application Details References for anti-ROCK2 antibody (ABIN968071) Images back to top
|Buffer||Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.|
|Precaution of Use||This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Storage Comment||Store undiluted at -20° C.|
References for anti-ROCK2 antibody (ABIN968071)Product Details anti-ROCK2 Antibody Target Details ROCK2 Application Details Handling Images back to top
|Product cited in:||
Begum, Sandu, Ito, Lohmann, Smolenski: "Active Rho kinase (ROK-alpha ) associates with insulin receptor substrate-1 and inhibits insulin signaling in vascular smooth muscle cells." in: The Journal of biological chemistry, Vol. 277, Issue 8, pp. 6214-22, 2002
Wang, Eto, Steers, Somlyo, Somlyo: "RhoA-mediated Ca2+ sensitization in erectile function." in: The Journal of biological chemistry, Vol. 277, Issue 34, pp. 30614-21, 2002
Adachi, Vita, Sannohe, Stafford, Alam, Kayaba, Chihara: "The functional role of rho and rho-associated coiled-coil forming protein kinase in eotaxin signaling of eosinophils." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 167, Issue 8, pp. 4609-15, 2001
Sahai, Olson, Marshall: "Cross-talk between Ras and Rho signalling pathways in transformation favours proliferation and increased motility." in: The EMBO journal, Vol. 20, Issue 4, pp. 755-66, 2001
Leung, Manser, Tan, Lim: "A novel serine/threonine kinase binding the Ras-related RhoA GTPase which translocates the kinase to peripheral membranes." in: The Journal of biological chemistry, Vol. 270, Issue 49, pp. 29051-4, 1996
ImagesProduct Details anti-ROCK2 Antibody Target Details ROCK2 Application Details Handling References for anti-ROCK2 antibody (ABIN968071) back to top