anti-Human CD40 Ligand antibody for Western Blotting

Recommended CD40 Ligand Antibody (supplied by: Log in to see )

Antigen
CD40 Ligand (CD40LG) Antibodies
  • CD154
  • cd40l
  • CD40L
  • HIGM1
  • IGM
  • IMD3
  • T-BAM
  • TNFSF5
  • TRAP
  • gp39
  • hCD40L
  • CD40-L
  • Cd40l
  • Ly-62
  • Ly62
  • Tnfsf5
  • CD40 ligand
  • TNF superfamily member 5
  • CD40LG
  • cd40l
  • Cd40lg
Epitope
Center
23
16
15
15
9
8
8
7
7
5
5
4
4
4
4
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine)
451
196
63
33
20
12
8
5
4
4
3
3
3
3
2
2
2
1
1
1
Host
Rabbit
253
207
87
31
23
17
3
1
Clonality
Polyclonal
Conjugate
This CD40 Ligand antibody is un-conjugated
76
54
42
15
7
7
7
6
6
5
5
4
4
4
4
4
4
3
3
3
3
3
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
Application
Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
346
296
151
106
72
43
40
35
34
22
13
12
9
9
8
6
5
4
3
3
2
2
2
1
1
1
1
1
Options
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Catalog No. ABIN2854749
$ 466.18
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
26.063175 ABIN5611141 ELISA FACS WB Mouse IgG1 AA 47-261 Log in to see 8H10F5 0
26.063175 ABIN5611142 ELISA FACS WB Mouse IgG2a AA 47-261 Log in to see 5A3A9 0
20.063175 ABIN3020937 IHC WB Rabbit IgG N-Term Log in to see 0
18.306385 ABIN3044319 IHC (p) WB Rabbit IgG AA 47-65, N-Term Log in to see Polyclonal 1
17.75679 ABIN2192075 IA FACS IP Neut WB Mouse Log in to see 24-31 2
17.063175 ABIN5668046 BR FACS WB Human IgG1, kappa Log in to see hu5c8 (Ruplizumab) 0
17.063175 ABIN634665 WB Rabbit Log in to see Polyclonal 0
16.25679 ABIN5668047 BR FACS WB Rabbit IgG, kappa Log in to see Chimeric 0
13.806385 ABIN390458 FACS IF IHC (p) WB Rabbit Ig Fraction AA 33-62, N-Term Log in to see Polyclonal 0
13.806385 ABIN1858301 ICC IHC WB Rabbit IgG AA 48-244 Log in to see Polyclonal 0
13.806385 ABIN394480 ELISA WB Mouse IgG2b kappa AA 101-204 Log in to see 2E2 0
13.806385 ABIN2896202 IF/ICC IHC IP WB Rabbit AA 48-244 Log in to see Polyclonal 0
13.25679 ABIN5564228 FACS Func WB Human IgG1 Log in to see hu5c8 0
13.25679 ABIN5619121 WB Rabbit Center Log in to see Polyclonal 0
13.25679 ABIN234628 ELISA WB Mouse IgG1 Log in to see 2B10 0
13.25679 ABIN288646 FACS IHC ELISA WB FITC Mouse IgG1 Log in to see 24-31 0
13.25679 ABIN288645 FACS IHC ELISA WB Biotin Mouse IgG1 Log in to see 24-31 0
13.25679 ABIN2136604 IHC (p) WB Rabbit IgG N-Term Log in to see Polyclonal 0
13.25679 ABIN636589 Inhibition ELISA WB Rabbit Log in to see Polyclonal 0
13.25679 ABIN636590 Inhibition ELISA WB Goat Log in to see Polyclonal 0

General

Antigen CD40 Ligand (CD40LG) Antibodies
Epitope Center
(23), (16), (15), (15), (9), (8), (8), (7), (7), (5), (5), (4), (4), (4), (4), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine)
(451), (196), (63), (33), (20), (12), (8), (5), (4), (4), (3), (3), (3), (3), (2), (2), (2), (1), (1), (1)
Host Rabbit
(253), (207), (87), (31), (23), (17), (3), (1)
Clonality
Polyclonal
Conjugate This CD40 Ligand antibody is un-conjugated
(76), (54), (42), (15), (7), (7), (7), (6), (6), (5), (5), (4), (4), (4), (4), (4), (4), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(346), (296), (151), (106), (72), (43), (40), (35), (34), (22), (13), (12), (9), (9), (8), (6), (5), (4), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1)
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Product Details anti-CD40 Ligand Antibody

Target Details CD40 Ligand Application Details Handling Images
Characteristics Rabbit Polyclonal antibody to TRAP (acid phosphatase 5, tartrate resistant)
TRAP antibody
Purification Purified by antigen-affinity chromatography.
Immunogen Recombinant protein encompassing a sequence within the center region of human TRAP. The exact sequence is proprietary.
Isotype IgG
Plasmids, Primers & others

Target Details CD40 Ligand

Product Details anti-CD40 Ligand Antibody Application Details Handling Images back to top
Antigen
Alternative Name TRAP (CD40LG Antibody Abstract)
Background This gene encodes an iron containing glycoprotein which catalyzes the conversion of orthophosphoric monoester to alcohol and orthophosphate. It is the most basic of the acid phosphatases and is the only form not inhibited by L(+)-tartrate.

Cellular Localization: Lysosome
Molecular Weight 37 kDa
Gene ID 54
Pathways NF-kappaB Signaling, Production of Molecular Mediator of Immune Response

Application Details

Product Details anti-CD40 Ligand Antibody Target Details CD40 Ligand Handling Images back to top
Application Notes Suggested dilution Reference ICC/IF Assay-dependent dilution IHC (Formalin-fixed paraffin-embedded sections) 1:100-1:1000* Western blot 1:500-1:3000* Not tested in other applications. *Optimal dilutions/concentrations should be determined by the researcher.Suggested dilutionReferenceICC/IFAssay-dependent dilution IHC (Formalin-fixed paraffin-embedded sections)1:100-1:1000* Western blot1:500-1:3000*
Comment

Positive Control: A431 , Molt-4 , NIH-3T3

Restrictions For Research Use only

Handling

Product Details anti-CD40 Ligand Antibody Target Details CD40 Ligand Application Details Images back to top
Format Liquid
Concentration 1 mg/mL
Buffer 1XPBS, 1 % BSA, 20 % Glycerol ( pH 7). 0.01 % Thimerosal was added as a preservative.
Preservative Thimerosal (Merthiolate)
Precaution of Use This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Images

Product Details anti-CD40 Ligand Antibody Target Details CD40 Ligand Application Details Handling back to top
Supplier Images
Immunohistochemistry (IHC) image for anti-CD40 Ligand (CD40LG) (Center) antibody (ABIN2854749) IHC-P Image TRAP antibody detects ACP5 protein at cytosol on mouse intestine by immun...
Western Blotting (WB) image for anti-CD40 Ligand (CD40LG) (Center) antibody (ABIN2854749) WB Image Sample(30 ug whole cell lysate) A:A431, B:MOLT4 , 12% SDS PAGE antibody dilu...
Western Blotting (WB) image for anti-CD40 Ligand (CD40LG) (Center) antibody (ABIN2854749) WB Image TRAP antibody detects TRAP protein by western blot analysis. Whole cell extr...
Western Blotting (WB) image for anti-CD40 Ligand (CD40LG) (Center) antibody (ABIN2854749) WB Image Sample (30 ug of whole cell lysate) A:NIH-3T3 10% SDS PAGE antibody diluted ...