anti-Human CD40 Ligand antibody for Western Blotting

Recommended CD40 Ligand Antibody (supplied by: Log in to see )

Antigen
CD40 Ligand (CD40LG) Antibodies
  • CD154
  • cd40l
  • CD40L
  • HIGM1
  • IGM
  • IMD3
  • T-BAM
  • TNFSF5
  • TRAP
  • gp39
  • hCD40L
  • CD40-L
  • Cd40l
  • Ly-62
  • Ly62
  • Tnfsf5
  • CD40 ligand
  • TNF superfamily member 5
  • CD40LG
  • cd40l
  • Cd40lg
Epitope
Middle Region
23
16
15
15
9
8
8
7
7
5
5
4
4
4
4
4
3
3
3
2
2
2
2
1
1
1
1
1
1
1
1
1
1
Reactivity
Cow (Bovine), Dog (Canine), Horse (Equine), Human, Mouse (Murine), Pig (Porcine), Rabbit, Rat (Rattus), Sheep (Ovine)
456
195
62
33
19
11
7
4
4
3
3
3
3
3
3
2
1
1
1
1
Host
Rabbit
256
207
86
31
25
17
3
1
Clonality
Polyclonal
Conjugate
This CD40 Ligand antibody is un-conjugated
76
59
42
15
7
7
7
6
6
5
5
4
4
4
4
4
4
3
3
3
3
3
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
Application
Immunohistochemistry (IHC), Western Blotting (WB)
347
298
154
107
71
43
41
37
34
23
13
12
9
9
8
6
5
4
3
3
2
2
2
1
1
1
1
1
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Catalog No. ABIN2778145
$ 289.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
3.0806255 ABIN3044319 IHC (p) WB Rabbit IgG AA 47-65, N-Term Log in to see Polyclonal 0
3.0806255 ABIN2705743 WB Rabbit Center Log in to see Polyclonal 0
3.0806255 ABIN390458 FACS IF IHC (p) WB Rabbit Ig Fraction AA 33-62, N-Term Log in to see Polyclonal 0
3.0806255 ABIN222966 WB Rabbit IgG Log in to see Polyclonal 0
3.0806255 ABIN4899210 CyTOF FACS WB Goat IgG AA 108-261 Log in to see Polyclonal 2
3.0806255 ABIN2854749 ICC IF IHC (p) WB Rabbit IgG Center Log in to see Polyclonal 0
3.0806255 ABIN1858301 ICC IHC WB Rabbit IgG AA 48-244 Log in to see Polyclonal 0
3.0806255 ABIN453437 EIA WB Rabbit N-Term Log in to see Polyclonal 2
3.0806255 ABIN453857 EIA FACS WB Rabbit C-Term Log in to see Polyclonal 2
3.0806255 ABIN394480 ELISA WB Mouse IgG2b kappa AA 101-204 Log in to see 2E2 0
3.0806255 ABIN4292978 IHC IHC (p) WB Rabbit IgG Log in to see Polyclonal 0
3.0806255 ABIN5668046 BR FACS WB Human IgG1, kappa Log in to see hu5c8 (Ruplizumab) 0
3.0806255 ABIN5611141 ELISA FACS WB Mouse IgG1 AA 47-261 Log in to see 8H10F5 0
3.0806255 ABIN5611142 ELISA FACS WB Mouse IgG2a AA 47-261 Log in to see 5A3A9 0
3.0806255 ABIN5945557 FACS ICC IF IHC IHC (p) WB Rabbit IgG N-Term Log in to see JM11-34 0
3.0806255 ABIN652211 FACS IHC (p) WB Rabbit Ig Fraction AA 181-207, C-Term Log in to see Polyclonal 0
3.0806255 ABIN223518 WB Rabbit IgG Log in to see Polyclonal 0
3.0806255 ABIN1871635 IF IHC WB Rabbit IgG Log in to see Polyclonal 0
3.0806255 ABIN1105902 IHC (p) WB Rabbit IgG N-Term Log in to see Polyclonal 0
3.0806255 ABIN1172349 ICC IHC WB Rabbit IgG AA 180-261 Log in to see Polyclonal 0

General

Antigen CD40 Ligand (CD40LG) Antibodies
Epitope Middle Region
(23), (16), (15), (15), (9), (8), (8), (7), (7), (5), (5), (4), (4), (4), (4), (4), (3), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Cow (Bovine), Dog (Canine), Horse (Equine), Human, Mouse (Murine), Pig (Porcine), Rabbit, Rat (Rattus), Sheep (Ovine)
(456), (195), (62), (33), (19), (11), (7), (4), (4), (3), (3), (3), (3), (3), (3), (2), (1), (1), (1), (1)
Host Rabbit
(256), (207), (86), (31), (25), (17), (3), (1)
Clonality
Polyclonal
Conjugate This CD40 Ligand antibody is un-conjugated
(76), (59), (42), (15), (7), (7), (7), (6), (6), (5), (5), (4), (4), (4), (4), (4), (4), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunohistochemistry (IHC), Western Blotting (WB)
(347), (298), (154), (107), (71), (43), (41), (37), (34), (23), (13), (12), (9), (9), (8), (6), (5), (4), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1)
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Product Details anti-CD40 Ligand Antibody

Target Details CD40 Ligand Application Details Handling Images
Blocking Peptide Blocking peptide for this product available: ABIN974677
Sequence ENSFEMQKGD QNPQIAAHVI SEASSKTTSV LQWAEKGYYT MSNNLVTLEN
Predicted Reactivity Cow: 100%, Dog: 100%, Horse: 100%, Human: 100%, Mouse: 83%, Pig: 100%, Rabbit: 92%, Rat: 85%, Sheep: 100%
Characteristics This is a rabbit polyclonal antibody against CD40LG. It was validated on Western Blot using a cell lysate as a positive control.
Purification Affinity Purified
Immunogen The immunogen is a synthetic peptide directed towards the middle region of human CD40LG
Plasmids, Primers & others

Target Details CD40 Ligand

Product Details anti-CD40 Ligand Antibody Application Details Handling Images back to top
Antigen
Alternative Name CD40LG (CD40LG Antibody Abstract)
Background CD40LG is expressed on the surface of T cells. It regulates B cell function by engaging CD40 on the B cell surface. A defect in this gene results in an inability to undergo immunoglobulin class switch and is associated with hyper-IgM syndrome.The protein encoded by this gene is expressed on the surface of T cells. It regulates B cell function by engaging CD40 on the B cell surface. A defect in this gene results in an inability to undergo immunoglobulin class switch and is associated with hyper-IgM syndrome. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications. PRIMARYREFSEQ_SPAN PRIMARY_IDENTIFIER PRIMARY_SPAN COMP 1-1375 BC071754.1 1-1375 1376-1599 X67878.1 1349-1572 1600-1834 BC071754.1 1596-1830
Alias Symbols: CD154, CD40L, HIGM1, IGM, IMD3, T-BAM, TNFSF5, TRAP, gp39, hCD40L
Protein Interaction Partner: UBC, TRAF2, TRAF1, CD40, BIRC3, BIRC2, CRYAB, IGHG1, CD5L, CD40LG, TP53, IGKC, RNF128, HPR, APOA1,
Protein Size: 261
Molecular Weight 29 kDa
Gene ID 959
NCBI Accession NM_000074, NP_000065
UniProt P29965
Pathways NF-kappaB Signaling, Production of Molecular Mediator of Immune Response

Application Details

Product Details anti-CD40 Ligand Antibody Target Details CD40 Ligand Handling Images back to top
Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 261 AA

Restrictions For Research Use only

Handling

Product Details anti-CD40 Ligand Antibody Target Details CD40 Ligand Application Details Images back to top
Format Liquid
Concentration Lot specific
Buffer Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Images

Product Details anti-CD40 Ligand Antibody Target Details CD40 Ligand Application Details Handling back to top
Supplier Images
Immunohistochemistry (IHC) image for anti-CD40 Ligand (CD40LG) (Middle Region) antibody (ABIN2778145) Immunohistochemistry with Thymus tissue at an antibody concentration of 5µg/ml using ...
Western Blotting (WB) image for anti-CD40 Ligand (CD40LG) (Middle Region) antibody (ABIN2778145) WB Suggested Anti-CD40LG Antibody Titration: 0.2-1 ug/ml ELISA Titer: 1:312500 Po...
Western Blotting (WB) image for anti-CD40 Ligand (CD40LG) (Middle Region) antibody (ABIN2778145) Host: Rabbit Target Name: CD40LG Sample Type: Human Fetal Muscle Lane A: Prima...