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|Antigen||CD40 Ligand (CD40LG) Antibodies|
|Reactivity||Mouse (Murine) Alternatives|
|Conjugate||This CD40 Ligand antibody is conjugated to FITC Alternatives|
Functional Studies (Func), Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (IHC)
|8 references available|
|Supplier||Log in to see|
Product Details anti-CD40 Ligand AntibodyTarget Details CD40 Ligand Application Details Handling References for anti-CD40 Ligand antibody (ABIN1302930) Images
|Specificity||The Armenian hamster monoclonal antibody MR-1 detects mouse CD154 / CD40L (CD40-ligand), a 39 kDa cell surface glycoprotein expressed predominantly on activated CD4+ lymphocytes.|
|Characteristics||The purified antibody is conjugated with Fluorescein isothiocyanate (FITC) under optimum conditions. The reagent is free of unconjugated FITC.|
|Immunogen||Activated mouse Th1 clone D1.6|
|Plasmids, Primers & others|
Target Details CD40 LigandProduct Details anti-CD40 Ligand Antibody Application Details Handling References for anti-CD40 Ligand antibody (ABIN1302930) Images back to top
|Alternative Name||CD40LG (CD40LG Antibody Abstract)|
|Background||CD154 / CD40L (CD40 ligand) is a member of the tumor necrosis factor family, and is expressed primarily on activated CD4+ lymphocytes, but also on mast cells, basophils, eosinophils and human dendritic cells. Its counter-receptor CD40 is expressed on antigen presenting cells, including dendritic cells, macrophages, and B cells, and also on fibroblasts. Triggering of CD40 by CD40L causes maturation of dendritic cells and upregulation of antigen presentation in functions of the MHC and costimulatory molecules. CD40L also functions as a direct stimulating factor for T cells. CD40L plays also roles e.g. in antibody class switching and modulation of apoptosis in the germinal center.|
|Pathways||NF-kappaB Signaling, Production of Molecular Mediator of Immune Response|
Application DetailsProduct Details anti-CD40 Ligand Antibody Target Details CD40 Ligand Handling References for anti-CD40 Ligand antibody (ABIN1302930) Images back to top
The reagent is designed for Flow Cytometry analysis. Suggested working concentration is 3 µg/mL. Indicated dilution is recommended starting point for use of this product. Working concentrations should be determined by the investigator.
Working concentrations should be determined by the investigator.
CD and Related Antigens & MHC (Rodent)
|Restrictions||For Research Use only|
HandlingProduct Details anti-CD40 Ligand Antibody Target Details CD40 Ligand Application Details References for anti-CD40 Ligand antibody (ABIN1302930) Images back to top
|Buffer||Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4|
|Precaution of Use||This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
Do not freeze.
Avoid prolonged exposure to light.
|Storage Comment||Store in the dark at 2-8 °C. Do not use after expiration date stamped on vial label. Short-term exposure to room temperature should not affect the quality of the reagent. However, if reagent is stored under any conditions other than those specified, the conditions must be verified by the user.|
References for anti-CD40 Ligand antibody (ABIN1302930)Product Details anti-CD40 Ligand Antibody Target Details CD40 Ligand Application Details Handling Images back to top
|Product cited in:||
Shin, Kennedy, Gorski, Tsuchiya, Koseki, Azuma, Yagita, Chen, Powell, Pardoll, Housseau: "Cooperative B7-1/2 (CD80/CD86) and B7-DC costimulation of CD4+ T cells independent of the PD-1 receptor." in: The Journal of experimental medicine, Vol. 198, Issue 1, pp. 31-8, 2003
Luzina, Atamas, Storrer, daSilva, Kelsoe, Papadimitriou, Handwerger: "Spontaneous formation of germinal centers in autoimmune mice." in: Journal of leukocyte biology, Vol. 70, Issue 4, pp. 578-84, 2001
Hwang, Nahm, Briles, Thomas, Purkerson: "Acquired, but not innate, immune responses to Streptococcus pneumoniae are compromised by neutralization of CD40L." in: Infection and immunity, Vol. 68, Issue 2, pp. 511-7, 2000
Lode, Xiang, Pertl, Förster, Schoenberger, Gillies, Reisfeld: "Melanoma immunotherapy by targeted IL-2 depends on CD4(+) T-cell help mediated by CD40/CD40L interaction." in: The Journal of clinical investigation, Vol. 105, Issue 11, pp. 1623-30, 2000
Howard, Miga, Vanderlugt, Dal Canto, Laman, Noelle, Miller: "Mechanisms of immunotherapeutic intervention by anti-CD40L (CD154) antibody in an animal model of multiple sclerosis." in: The Journal of clinical investigation, Vol. 103, Issue 2, pp. 281-90, 1999
Salgado, Nakamura, Sugaya, Tada, Asahina, Koyama, Irie, Tamaki: "Functional CD40 ligand is expressed on epidermal Langerhans cells." in: Journal of leukocyte biology, Vol. 66, Issue 2, pp. 281-5, 1999
Wu, Zhou, Zheng, Liu: "CD28-independent induction of T helper cells and immunoglobulin class switches requires costimulation by the heat-stable antigen." in: The Journal of experimental medicine, Vol. 187, Issue 7, pp. 1151-6, 1998
Noelle, Roy, Shepherd, Stamenkovic, Ledbetter, Aruffo: "A 39-kDa protein on activated helper T cells binds CD40 and transduces the signal for cognate activation of B cells." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 89, Issue 14, pp. 6550-4, 1992