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|Antigen||Interleukin 1 Receptor Antagonist (IL1RN) Antibodies|
|Conjugate||This IL1RN antibody is un-conjugated Alternatives|
Flow Cytometry (FACS), Immunohistochemistry (IHC), Radioimmunoassay (RIA), ELISA, Western Blotting (WB)
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Product Details anti-IL1RN AntibodyTarget Details IL1RN Application Details Handling Images
|Specificity||Recombinant human sIL-1Ra produced in E. coli.|
Recombinant human IL1RN / IL-1RA.
Type of Immunogen: Recombinant protein
Target Details IL1RNProduct Details anti-IL1RN Antibody Application Details Handling Images back to top
|Alternative Name||IL1RN (IL1RN Antibody Abstract)|
Name/Gene ID: IL1RN
Synonyms: IL1RN, Anakinra, DIRA, IL-1ra, IL-1RN, IL1F3, IL1RA, MVCD4, IL1 inhibitor, IL1RN (IL1F3), IRAP, ICIL-1RA, IL-1ra3
|Pathways||NF-kappaB Signaling, Hormone Transport|
Application DetailsProduct Details anti-IL1RN Antibody Target Details IL1RN Handling Images back to top
Approved: ELISA (1:500 - 1:1000), Flo, IHC, RIA, WB
Usage: This IgG fraction antibody of anti-Human IL-1 Receptor Antagonist has been tested for use in neutralizations, ELISA, radioimmunoassays, western blotting, immunohistochemistry and flow cytometry. Reactivity in other immunoassays is unknown. This product has been assayed by immunoblot using HRP Goat-anti-Rabbit IgG [H&L] (catalog no. LS-C60884) and TMB as a substrate. A working dilution range of 1:400 to 1:800 is suggested for this application. This product has been assayed by ELISA against IL-1 Receptor Antagonist using HRP Conjugated Anti-Rabbit IgG [H&L] (Goat) (catalog no. LS-C60884) and ABTS as a substrate for 30 minutes at room temperature. A working dilution range of 1:500 to 1:1000 is suggested for this product. For use in ELISA formats, this antibody is best used as the second antibody in combination with a monoclonal antibody as a capture antibody. This product has been assayed by immunohistochemistry. A dilution of 1:400 or greater is suggested for this immunoassay. Either paraffin fixation or cryofixation can be used for immunohistochemistry. This product has been assayed by radioimmunoassay against antigen. A dilution of 1:8000 is suggested for this immunoassay.
|Restrictions||For Research Use only|
HandlingProduct Details anti-IL1RN Antibody Target Details IL1RN Application Details Images back to top
|Buffer||0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.01 % sodium azide.|
|Precaution of Use||This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid cycles of freezing and thawing.|
|Storage Comment||Store vial at -20 °C prior to opening. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20 °C or below. Avoid cycles of freezing and thawing.|