anti-Horse (Equine) Insulin antibody for Immunohistochemistry (Frozen Sections)

Recommended Insulin Antibody (supplied by: Log in to see )

Antigen
Insulin (INS) Antibodies
  • ins1
  • xins
  • ins1-a
  • Insulin
  • IDDM2
  • ILPR
  • IRDN
  • MODY10
  • AA986540
  • Ins-2
  • InsII
  • Mody
  • Mody4
  • proinsulin
  • zgc:109842
  • insulin
  • insulin precursor
  • insulin II
  • preproinsulin
  • ins
  • PIN
  • INS
  • Ins
  • INS-IGF2
  • Ins2
Reactivity
Cat (Feline), Cow (Bovine), Dog (Canine), Guinea Pig, Horse (Equine), Human, Pig (Porcine), Rabbit, Rat (Rattus), Sheep (Ovine)
876
391
263
257
206
119
49
5
5
5
4
4
3
2
2
1
1
1
1
1
1
1
Host
Mouse
708
179
53
16
3
1
Clonality
Monoclonal
Conjugate
This Insulin antibody is un-conjugated
47
38
37
23
22
22
22
17
17
14
14
14
14
14
14
14
14
10
9
8
8
8
5
5
5
5
5
5
2
1
Application
Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
507
352
229
205
189
181
147
124
66
64
33
26
15
11
8
6
5
4
4
3
3
2
2
1
1
1
1
1
Supplier
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Pubmed
2 references available
Catalog No. ABIN114751
$ 308.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN114750 IHC (fro) IHC (p) Mouse IgG1 Log in to see K36aC10 0

General

Antigen Insulin (INS) Antibodies
Reactivity Cat (Feline), Cow (Bovine), Dog (Canine), Guinea Pig, Horse (Equine), Human, Pig (Porcine), Rabbit, Rat (Rattus), Sheep (Ovine)
(876), (391), (263), (257), (206), (119), (49), (5), (5), (5), (4), (4), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Host Mouse
(708), (179), (53), (16), (3), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This Insulin antibody is un-conjugated
(47), (38), (37), (23), (22), (22), (22), (17), (17), (14), (14), (14), (14), (14), (14), (14), (14), (10), (9), (8), (8), (8), (5), (5), (5), (5), (5), (5), (2), (1)
Application Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
(507), (352), (229), (205), (189), (181), (147), (124), (66), (64), (33), (26), (15), (11), (8), (6), (5), (4), (4), (3), (3), (2), (2), (1), (1), (1), (1), (1)
Pubmed 2 references available
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Product Details anti-Insulin Antibody

Target Details Insulin Application Details Handling References for anti-Insulin antibody (ABIN114751) Images
Specificity This antibody recognizes purified Insulin from the pancreas of Human, Bovine, Horse, Sheep as well as Proinsulin from Human. Cross reaction has been observed with insulin containing cells in fixed sections of pancreas from Human, Porcine, Dog, Rabbit, Bovine, Sheep, Rat, Guinea Pig and Cat.
Cellular Localization: Cytoplasmic.Cellular Localization
Purification Supernatant
Immunogen Human insulin.
Clone K36aC10
Isotype IgG1

Target Details Insulin

Product Details anti-Insulin Antibody Application Details Handling References for anti-Insulin antibody (ABIN114751) Images back to top
Antigen
Alternative Name Insulin (INS Antibody Abstract)
Background Insulin is one of the major regulatory hormones of intermediate metabolism throughout the body. The biological actions of this hormone involve integration of carbohydrate, protein, and lipid metabolism. Insulin enhances membrane transport of glucose, amino acids, and certain ions. It also promotes glycogen storage, formation of triglycerides and synthesis of proteins and nucleic acids. Immunocytochemical investigations have localized insulin in the B cells of pancreatic islets of Langerhans. Deficiency of insulin results in diabetes mellitus, one of the leading causes of morbidity and mortality in the general population. Insulin is also present in tumors of B cell origin such as insulinoma.Synonyms: INS
Gene ID 3630
NCBI Accession NP_000198
UniProt P01308
Pathways NF-kappaB Signaling, RTK Signaling, Positive Regulation of Peptide Hormone Secretion, Peptide Hormone Metabolism, Hormone Activity, Carbohydrate Homeostasis, ER-Nucleus Signaling, Regulation of Carbohydrate Metabolic Process, Feeding Behaviour, Autophagy, Negative Regulation of intrinsic apoptotic Signaling, Brown Fat Cell Differentiation, Positive Regulation of fat Cell Differentiation

Application Details

Product Details anti-Insulin Antibody Target Details Insulin Handling References for anti-Insulin antibody (ABIN114751) Images back to top
Application Notes Immunohistochemistry on Formalin/Paraffin Sections: Use a dilution of 1/25-1/50 in anABC method (30 minutes at room temperature). Recommended Positive Control: Pancreas.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-Insulin Antibody Target Details Insulin Application Details References for anti-Insulin antibody (ABIN114751) Images back to top
Format Liquid
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

References for anti-Insulin antibody (ABIN114751)

Product Details anti-Insulin Antibody Target Details Insulin Application Details Handling Images back to top
Background publications

Madsen: "Proinsulin-specific monoclonal antibodies. Immunocytochemical application as beta-cell markers and as probes for conversion." in: Diabetes, Vol. 36, Issue 10, pp. 1203-11, 1987

Keilacker, Dietz, Witt, Woltanski, Berling, Ziegler: "Kinetic properties of monoclonal insulin antibodies." in: Biomedica biochimica acta, Vol. 45, Issue 8, pp. 1093-102, 1986