anti-Human Insulin antibody for Immunohistochemistry (Formalin-fixed Sections)

Recommended Insulin Antibody (supplied by: Log in to see )

Antigen
Insulin (INS) Antibodies
  • ins1
  • xins
  • ins1-a
  • Insulin
  • IDDM2
  • ILPR
  • IRDN
  • MODY10
  • AA986540
  • Ins-2
  • InsII
  • Mody
  • Mody4
  • proinsulin
  • zgc:109842
  • insulin
  • insulin precursor
  • insulin II
  • preproinsulin
  • ins
  • PIN
  • INS
  • Ins
  • INS-IGF2
  • Ins2
Reactivity
Cow (Bovine), Human, Mouse (Murine), Pig (Porcine)
987
495
380
373
235
188
47
9
7
6
5
5
4
2
2
1
1
1
1
1
1
1
Host
Mouse
817
196
50
16
3
1
Clonality
Monoclonal
Conjugate
This Insulin antibody is conjugated to CF®770
53
43
38
28
27
22
22
19
17
17
15
14
14
14
14
14
14
14
9
9
9
8
8
8
5
5
5
5
5
5
5
5
5
5
5
5
5
5
5
5
5
5
4
2
1
Application
Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))
501
390
278
278
250
235
189
118
105
67
66
31
26
23
21
8
7
6
5
4
4
3
2
1
1
1
1
1
1
Supplier
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Catalog No. ABIN6157345
$ 209.23
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN6157351 FACS IF IHC (f) CF®488A Mouse IgG1, kappa Log in to see 2D11-H5 0
1 ABIN6157347 FACS IF IHC (f) CF®680 Mouse IgG1, kappa Log in to see 2D11-H5 0
1 ABIN6157389 FACS IF IHC (f) CF®488A Mouse IgG Log in to see E2-E3 2D11-H5 0
1 ABIN6157417 FACS IF IHC (f) CF®660R Mouse IgG1, kappa Log in to see E2-E3 0
1 ABIN6157419 FACS IF IHC (f) CF®568 Mouse IgG1, kappa Log in to see E2-E3 0
1 ABIN6157421 FACS IF IHC (f) CF®770 Mouse IgG1, kappa Log in to see E2-E3 0
1 ABIN6157425 FACS IF IHC (f) CF®680R Mouse IgG1, kappa Log in to see E2-E3 0
1 ABIN6157427 FACS IF IHC (f) CF®488A Mouse IgG1, kappa Log in to see E2-E3 0
1 ABIN6157443 FACS IF IHC (f) CF®405S Mouse IgG1, kappa Log in to see IRDN-794 0
1 ABIN6157447 FACS IF IHC (f) CF®640R Mouse IgG1, kappa Log in to see IRDN-794 0
1 ABIN6157453 FACS IF IHC (f) CF®555 Mouse IgG1, kappa Log in to see IRDN-794 0
1 ABIN6157457 FACS IF IHC (f) CF®568 Mouse IgG1, kappa Log in to see IRDN-794 0
1 ABIN6157489 FACS IF IHC (f) IHC (p) ISt CF®647 Mouse IgG1, kappa Log in to see IRDN-805 0
1 ABIN6157495 FACS IF IHC (f) IHC (p) ISt CF®568 Mouse IgG1, kappa Log in to see IRDN-805 0
1 ABIN6157481 FACS IF IHC (f) IHC (p) ISt CF®405S Mouse IgG1, kappa Log in to see IRDN-805 0
1 ABIN6157487 FACS IF IHC (f) IHC (p) ISt CF®543 Mouse IgG1, kappa Log in to see IRDN-805 0
1 ABIN6157497 FACS IF IHC (f) IHC (p) ISt CF®770 Mouse IgG1, kappa Log in to see IRDN-805 0
1 ABIN6157499 FACS IF IHC (f) IHC (p) ISt CF®680 Mouse IgG1, kappa Log in to see IRDN-805 0
1 ABIN6157505 FACS IF IHC (f) IHC (p) ISt CF®594 Mouse IgG1, kappa Log in to see IRDN-805 0
1 ABIN6157339 FACS IF IHC (f) CF®555 Mouse IgG1, kappa Log in to see 2D11-H5 0

General

Antigen Insulin (INS) Antibodies
Reactivity Cow (Bovine), Human, Mouse (Murine), Pig (Porcine)
(987), (495), (380), (373), (235), (188), (47), (9), (7), (6), (5), (5), (4), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Host Mouse
(817), (196), (50), (16), (3), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This Insulin antibody is conjugated to CF®770
(53), (43), (38), (28), (27), (22), (22), (19), (17), (17), (15), (14), (14), (14), (14), (14), (14), (14), (9), (9), (9), (8), (8), (8), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (4), (2), (1)
Application Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))
(501), (390), (278), (278), (250), (235), (189), (118), (105), (67), (66), (31), (26), (23), (21), (8), (7), (6), (5), (4), (4), (3), (2), (1), (1), (1), (1), (1), (1)
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Product Details anti-Insulin Antibody

Target Details Insulin Application Details Handling Images
Purpose Mouse Monoclonal anti-Insulin (2D11-H5), CF770 Conjugate
Characteristics This antibody recognizes a polypeptide which is identified as insulin, a 51-amino acid polypeptide composed of A and B chains connected through the C-peptide. Proinsulin, which has very little biological activity, is cleaved by proteases within its cell of origin into the insulin molecule and the C-terminal basic residue. Insulin enhances membrane transport of glucose, amino acids, and certain ions. It also promotes glycogen storage, formation of triglycerides, and synthesis of proteins and nucleic acids. Deficiency of insulin results in diabetes mellitus. The main storage site for insulin is the pancreatic islets. Antibodies to insulin are important as beta-cell and insulinoma marker. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
Immunogen Purified pig insulin, conjugated to KLH
Clone 2D11-H5
Isotype IgG1, kappa
Plasmids, Primers & others

Target Details Insulin

Product Details anti-Insulin Antibody Application Details Handling Images back to top
Antigen
Alternative Name Insulin (INS Antibody Abstract)
Background IDDM2, ILPR, IRDN, MODY10, Proinsulin
Molecular Weight 6 kDa
Gene ID 3630, 272259
UniProt P01308
Pathways NF-kappaB Signaling, RTK Signaling, Positive Regulation of Peptide Hormone Secretion, Peptide Hormone Metabolism, Hormone Activity, Carbohydrate Homeostasis, ER-Nucleus Signaling, Regulation of Carbohydrate Metabolic Process, Feeding Behaviour, Autophagy, Negative Regulation of intrinsic apoptotic Signaling, Brown Fat Cell Differentiation, Positive Regulation of fat Cell Differentiation

Application Details

Product Details anti-Insulin Antibody Target Details Insulin Handling Images back to top
Application Notes Immunohistology formalin-fixed 0.1-0.2 μg/mL
  • Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
  • Flow Cytometry 0.5-1 μg/million cells/0.1 mL
  • Immunofluorescence 1-2 μg/mL
  • Optimal dilution for a specific application should be determined by user
Comment

MIA PaCa-2 cells or Pancreas

Restrictions For Research Use only

Handling

Product Details anti-Insulin Antibody Target Details Insulin Application Details Images back to top
Format Liquid
Concentration 100 μg/mL
Buffer PBS/0.1 % BSA/0.05 % azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Protect from light