anti-Human Insulin antibody for Immunohistochemistry (Paraffin-embedded Sections)

Recommended Insulin Antibody (supplied by: Log in to see )

Antigen
Insulin (INS) Antibodies
  • ins1
  • xins
  • ins1-a
  • Insulin
  • IDDM2
  • ILPR
  • IRDN
  • MODY10
  • AA986540
  • Ins-2
  • InsII
  • Mody
  • Mody4
  • proinsulin
  • zgc:109842
  • insulin
  • insulin precursor
  • insulin II
  • preproinsulin
  • ins
  • PIN
  • INS
  • Ins
  • INS-IGF2
  • Ins2
Reactivity
Human
980
491
379
369
232
185
47
9
7
6
5
5
4
2
2
1
1
1
1
1
1
1
Host
Mouse
810
196
50
16
3
1
Clonality
Monoclonal
Conjugate
This Insulin antibody is conjugated to HRP
53
42
38
28
27
22
22
19
17
17
15
14
14
14
14
14
14
14
9
9
9
8
8
8
5
5
5
5
5
5
5
5
5
5
5
5
5
5
5
5
5
5
5
2
1
Application
ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
500
387
279
272
245
233
189
118
106
67
66
31
26
23
21
8
7
6
5
4
4
3
2
1
1
1
1
1
1
Supplier
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Catalog No. ABIN337134
$ 484.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
13.042537 ABIN214027 ELISA IHC IHC (p) WB Mouse IgG1, kappa Log in to see 2D11-H5 0
13.042537 ABIN316680 EIA IHC (p) Mouse IgG1 Log in to see 2D11 0
13.042537 ABIN260218 IHC (p) IHC ELISA Mouse IgG1 Log in to see 2D11 0
13.042537 ABIN396953 IHC IHC (p) Mouse IgG1, kappa Log in to see 0
13.042537 ABIN461989 ELISA IHC IHC (p) Mouse IgG1 Log in to see 0
13.042537 ABIN269653 DB ICC IF IHC (p) IHC RIA Mouse IgG1 Log in to see K36aC10 0
13.042537 ABIN4326017 IHC IHC (p) WB Rabbit IgG Log in to see Polyclonal 2
13.042537 ABIN337249 FACS ICC IF IHC IHC (p) Rabbit IgG Log in to see Polyclonal 0
13.042537 ABIN448377 IHC (p) IHC Mouse IgG1 Log in to see ISL-8J 0
13.042537 ABIN261237 ELISA IHC (p) Mouse IgG1 kappa Log in to see 8E2 0
13.042537 ABIN498557 IHC (p) Rabbit Log in to see Polyclonal 0
13.042537 ABIN4326016 FACS ICC IF IHC (p) Rabbit IgG Log in to see Polyclonal 0
1 ABIN3043651 IHC (p) Mouse IgG1 Log in to see K36AC10 20
1 ABIN372838 EIA FACS IF IHC (p) Rabbit IgG Log in to see Polyclonal 3
1 ABIN1804388 ELISA IHC IHC (fro) IHC (p) Mouse IgG1 Log in to see D3E7 (5B6-6) 0
1 ABIN768892 ELISA IHC IHC (fro) IHC (p) Mouse IgG1 Log in to see D3E7 0
1 ABIN1804387 ELISA IHC IHC (fro) IHC (p) Mouse IgG1 Log in to see E2E3 0
1 ABIN112214 EIA IHC (fro) IHC (p) Mouse IgG1 Log in to see D3E7 (5B6-6) 1
1 ABIN255726 IHC (fro) IHC (p) IHC ELISA Mouse IgG1 Log in to see D3E7 (5B6-6) 0
1 ABIN956886 IHC IHC (p) Mouse IgG1, kappa Log in to see INS04 0

General

Antigen Insulin (INS) Antibodies
Reactivity Human
(980), (491), (379), (369), (232), (185), (47), (9), (7), (6), (5), (5), (4), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Host Mouse
(810), (196), (50), (16), (3), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This Insulin antibody is conjugated to HRP
(53), (42), (38), (28), (27), (22), (22), (19), (17), (17), (15), (14), (14), (14), (14), (14), (14), (14), (9), (9), (9), (8), (8), (8), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (2), (1)
Application ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
(500), (387), (279), (272), (245), (233), (189), (118), (106), (67), (66), (31), (26), (23), (21), (8), (7), (6), (5), (4), (4), (3), (2), (1), (1), (1), (1), (1), (1)
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Product Details anti-Insulin Antibody

Target Details Insulin Application Details Handling Images
Brand IHC-plus™
Specificity Recognizes human Insulin. Species cross-reactivity: human proinsulin, bovine insulin (30 % ) and porcine insulin. Does not cross-react with free C-peptide.
Purification Protein A purified
Immunogen Purified human insulin.

Type of Immunogen: Purified protein
Clone 8E2
Isotype IgG1, kappa
Plasmids, Primers & others

Target Details Insulin

Product Details anti-Insulin Antibody Application Details Handling Images back to top
Antigen
Alternative Name Insulin (INS Antibody Abstract)
Background Name/Gene ID: INS
Family: Hormone

Synonyms: INS, IRDN, IDDM2, Preproinsulin, ILPR, Insulin, MODY10, Proinsulin
Gene ID 3630
UniProt P01308
Pathways NF-kappaB Signaling, RTK Signaling, Positive Regulation of Peptide Hormone Secretion, Peptide Hormone Metabolism, Hormone Activity, Carbohydrate Homeostasis, ER-Nucleus Signaling, Regulation of Carbohydrate Metabolic Process, Feeding Behaviour, Autophagy, Negative Regulation of intrinsic apoptotic Signaling, Brown Fat Cell Differentiation, Positive Regulation of fat Cell Differentiation

Application Details

Product Details anti-Insulin Antibody Target Details Insulin Handling Images back to top
Application Notes Approved: ELISA, IHC, IHC-P (10 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 10 μg/mL. Recommended as a detection antibody for ELISA and in sandwich assay with clone 3A6 as the capture antibody. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
Comment

Target Species of Antibody: Human

Restrictions For Research Use only

Handling

Product Details anti-Insulin Antibody Target Details Insulin Application Details Images back to top
Format Liquid
Concentration Lot specific
Buffer PBS,  pH 7.4, 0.05 % Proclin 300
Preservative ProClin
Precaution of Use This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid freeze-thaw cycles and prolonged exposure to light.
Storage 4 °C,-20 °C
Storage Comment Short term: store at 4°C. Long term: aliquot and store -20°C. Avoid freeze-thaw cycles. Protect from light.

Images

Product Details anti-Insulin Antibody Target Details Insulin Application Details Handling back to top
Supplier Images
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-Insulin (INS) antibody (HRP) (ABIN337134) Human Pancreas (formalin-fixed, paraffin-embedded) stained with INS antibody ABIN3371...