anti-Rat (Rattus) Insulin antibody for Flow Cytometry

Recommended Insulin Antibody

Antigen
Insulin (INS) Antibodies
  • ins1
  • xins
  • ins1-a
  • Insulin
  • IDDM2
  • ILPR
  • IRDN
  • MODY10
  • AA986540
  • Ins-2
  • InsII
  • Mody
  • Mody4
  • proinsulin
  • zgc:109842
  • insulin
  • insulin precursor
  • insulin II
  • preproinsulin
  • ins
  • PIN
  • INS
  • Ins
  • INS-IGF2
  • Ins2
Reactivity
Cow (Bovine), Human, Mouse (Murine), Pig (Porcine), Rat (Rattus)
717
274
245
219
197
108
9
8
6
6
6
4
3
2
2
2
2
2
2
1
1
1
1
Host
Mouse
538
213
50
4
2
1
Clonality
Monoclonal
Conjugate
This Insulin antibody is conjugated to APC
39
29
24
15
13
12
8
8
8
8
8
8
5
5
5
5
5
5
5
5
5
5
5
5
5
5
5
5
5
5
5
2
1
Application
Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))
335
227
193
165
158
126
106
66
57
33
30
30
23
21
21
7
5
4
4
4
3
2
2
2
1
1
1
1
1
Options

Available images

Catalog No. ABIN6157469
$ 441.85
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Clonality References Details
7 ABIN6157477 FACS IF IHC (f) PE Mouse IgG1, kappa IRDN-794 0
7 ABIN6157476 FACS IF IHC (f) PerCP Mouse IgG1, kappa IRDN-794 0
7 ABIN6157480 FACS IF IHC (f) Mouse IgG1, kappa IRDN-794 0
7 ABIN337249 FACS ICC IF IHC IHC (p) Rabbit IgG Polyclonal 0
4 ABIN1735946 ICC FACS IF IHC (p) ELISA Rabbit IgG Polyclonal 0
1 ABIN6157443 FACS IF IHC (f) CF®405S Mouse IgG1, kappa IRDN-794 0
1 ABIN6157453 FACS IF IHC (f) CF®555 Mouse IgG1, kappa IRDN-794 0
1 ABIN6157455 FACS IF IHC (f) CF®660R Mouse IgG1, kappa IRDN-794 0
1 ABIN6157461 FACS IF IHC (f) CF®680 Mouse IgG1, kappa IRDN-794 0
1 ABIN6157463 FACS IF IHC (f) CF®680R Mouse IgG1, kappa IRDN-794 0
1 ABIN6157445 FACS IF IHC (f) CF405M Mouse IgG1, kappa IRDN-794 0
1 ABIN6157465 FACS IF IHC (f) CF®488A Mouse IgG1, kappa IRDN-794 0
1 ABIN6157449 FACS IF IHC (f) CF®543 Mouse IgG1, kappa IRDN-794 0
1 ABIN6157457 FACS IF IHC (f) CF®568 Mouse IgG1, kappa IRDN-794 0
1 ABIN6157467 FACS IF IHC (f) CF®594 Mouse IgG1, kappa IRDN-794 0
1 ABIN6157447 FACS IF IHC (f) CF®640R Mouse IgG1, kappa IRDN-794 0
1 ABIN6157451 FACS IF IHC (f) CF®647 Mouse IgG1, kappa IRDN-794 0
1 ABIN6157459 FACS IF IHC (f) CF®770 Mouse IgG1, kappa IRDN-794 0
1 ABIN6157478 FACS IF IHC (f) Mouse IgG1, kappa IRDN-794 0
1 ABIN6157470 FACS IF IHC (f) Alkaline Phosphatase (AP) Mouse IgG1, kappa IRDN-794 0

General

Antigen Insulin (INS) Antibodies
Reactivity Cow (Bovine), Human, Mouse (Murine), Pig (Porcine), Rat (Rattus)
(717), (274), (245), (219), (197), (108), (9), (8), (6), (6), (6), (4), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1)
Host Mouse
(538), (213), (50), (4), (2), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This Insulin antibody is conjugated to APC
(39), (29), (24), (15), (13), (12), (8), (8), (8), (8), (8), (8), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (2), (1)
Application Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))
(335), (227), (193), (165), (158), (126), (106), (66), (57), (33), (30), (30), (23), (21), (21), (7), (5), (4), (4), (4), (3), (2), (2), (2), (1), (1), (1), (1), (1)
Supplier

Product Details anti-Insulin Antibody

Target Details Insulin Application Details Handling Images
Purpose Mouse Monoclonal anti-Insulin (IRDN/794), APC Conjugate
Characteristics This antibody recognizes a polypeptide which is identified as insulin, a 51-amino acid polypeptide composed of A and B chains connected through the C-peptide. Proinsulin, which has very little biological activity, is cleaved by proteases within its cell of origin into the insulin molecule and the C-terminal basic residue. Insulin enhances membrane transport of glucose, amino acids, and certain ions. It also promotes glycogen storage, formation of triglycerides, and synthesis of proteins and nucleic acids. Deficiency of insulin results in diabetes mellitus. The main storage site for insulin is the pancreatic islets. Antibodies to insulin are important as beta-cell and insulinoma marker. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
Immunogen Recombinant INS protein
Clone IRDN-794
Isotype IgG1, kappa
Plasmids, Primers & others

Target Details Insulin

Product Details anti-Insulin Antibody Application Details Handling Images back to top
Antigen
Alternative Name Insulin (INS Antibody Abstract)
Background IDDM2, ILPR, IRDN, MODY10, Proinsulin
Molecular Weight 6 kDa
Gene ID 3630, 272259
UniProt P01308
Pathways NF-kappaB Signaling, RTK Signaling, Positive Regulation of Peptide Hormone Secretion, Peptide Hormone Metabolism, Hormone Activity, Carbohydrate Homeostasis, ER-Nucleus Signaling, Regulation of Carbohydrate Metabolic Process, Feeding Behaviour, Autophagy, Negative Regulation of intrinsic apoptotic Signaling, Brown Fat Cell Differentiation, Positive Regulation of fat Cell Differentiation

Application Details

Product Details anti-Insulin Antibody Target Details Insulin Handling Images back to top
Application Notes Immunohistology formalin-fixed 0.1-0.2 μg/mL
  • Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
  • Flow Cytometry 0.5-1 μg/million cells/0.1 mL
  • Immunofluorescence 1-2 μg/mL
  • Optimal dilution for a specific application should be determined by user
Comment

MIA PaCa-2 cells or Pancreas

Restrictions For Research Use only

Handling

Product Details anti-Insulin Antibody Target Details Insulin Application Details Images back to top
Format Liquid
Concentration 100 μg/mL
Buffer PBS/0.1 % BSA/0.05 % azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Images

Product Details anti-Insulin Antibody Target Details Insulin Application Details Handling back to top
Supplier Images
 image for anti-Insulin (INS) antibody (APC) (ABIN6157469) anti-Insulin (INS) antibody (APC)
 image for anti-Insulin (INS) antibody (APC) (ABIN6157469) anti-Insulin (INS) antibody (APC) (Image 2)