anti-Mouse (Murine) PKC zeta antibody for Immunohistochemistry

Recommended PKC zeta Antibody (supplied by: Log in to see )

Antigen
Protein Kinase C, zeta (PRKCZ) Antibodies
  • TPKC
  • PKCzeta
  • prkcz-A
  • pkc-zeta
  • PRKCZ
  • pkc2
  • PKC-ZETA
  • PKC2
  • AI098070
  • C80388
  • Pkcz
  • R74924
  • aPKCzeta
  • zetaPKC
  • 14-3-3-zetaisoform
  • r14-3-3
  • pkcz
  • si:ch211-150o23.4
  • protein kinase C zeta L homeolog
  • protein kinase C zeta
  • protein kinase C, zeta
  • prkcz.L
  • PRKCZ
  • prkcz
  • Prkcz
Epitope
pThr410
44
30
19
16
11
8
7
6
5
4
4
4
3
3
3
3
3
2
2
2
2
2
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
194
128
110
11
9
8
4
4
4
2
2
2
2
2
Host
Rabbit
221
6
1
Clonality
Polyclonal
Conjugate
This PKC zeta antibody is un-conjugated
10
9
7
4
4
4
1
1
1
1
1
1
1
1
1
1
1
Application
ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blotting (WB)
173
116
104
43
36
18
13
13
11
3
3
2
2
2
1
1
Supplier
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Catalog No. ABIN6256558
$ 390.77
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
18.992594 ABIN6264247 ELISA ICC IF IHC WB Rabbit IgG Log in to see Polyclonal 0
18.760654 ABIN3186477 ELISA IF IHC WB Rabbit IgG Thr33 Log in to see Polyclonal 0
18.760654 ABIN3182128 ELISA IF IHC WB Rabbit IgG pThr410 Log in to see Polyclonal 0
15.760654 ABIN3186476 ELISA IF IHC WB Rabbit IgG Thr322 Log in to see Polyclonal 0
15.760654 ABIN3182129 ELISA IHC WB Rabbit IgG pThr560 Log in to see Polyclonal 0
12.992594 ABIN2705134 IC IF IP IHC WB Rabbit C-Term Log in to see Polyclonal 0
12.992594 ABIN1531192 IF IHC ELISA WB Rabbit IgG AA 376-425, pThr410 Log in to see Polyclonal 0
12.992594 ABIN1532525 IF IHC ELISA WB Rabbit IgG AA 526-575 Log in to see Polyclonal 0
12.992594 ABIN1532208 IF IHC ELISA WB Rabbit IgG AA 376-425 Log in to see Polyclonal 0
12.992594 ABIN6256579 ELISA ICC IF IHC WB Rabbit IgG pThr560 Log in to see Polyclonal 0
12.992594 ABIN1847212 ELISA IF/ICC IHC WB Rabbit pThr410 Log in to see Polyclonal 0
12.760654 ABIN5622152 IHC WB Rabbit Log in to see Polyclonal 0
12.760654 ABIN2149132 IF IHC ELISA WB Rabbit IgG pThr410 Log in to see Polyclonal 0
12.760654 ABIN2149134 IHC ELISA WB Rabbit IgG pThr560 Log in to see Polyclonal 0
12.760654 ABIN966843 IHC Rabbit pThr410 Log in to see 0
12.760654 ABIN966844 IHC Rabbit Log in to see Polyclonal 0
9.992594 ABIN1531510 IHC ELISA WB Rabbit IgG AA 526-575, pThr560 Log in to see Polyclonal 0
7 ABIN1860238 ICC IHC WB Rabbit IgG AA 254-484 Log in to see Polyclonal 0
7 ABIN2921988 IF/ICC IHC IP WB Rabbit AA 254-484 Log in to see Polyclonal 0
6.992594 ABIN1870530 IHC Rabbit IgG pThr410 Log in to see Polyclonal 0

General

Antigen Protein Kinase C, zeta (PRKCZ) Antibodies
Epitope pThr410
(44), (30), (19), (16), (11), (8), (7), (6), (5), (4), (4), (4), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(194), (128), (110), (11), (9), (8), (4), (4), (4), (2), (2), (2), (2), (2)
Host Rabbit
(221), (6), (1)
Clonality
Polyclonal
Conjugate This PKC zeta antibody is un-conjugated
(10), (9), (7), (4), (4), (4), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blotting (WB)
(173), (116), (104), (43), (36), (18), (13), (13), (11), (3), (3), (2), (2), (2), (1), (1)
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Product Details anti-PKC zeta Antibody

Target Details PKC zeta Application Details Handling Images
Specificity Phospho-PKC zeta (Thr410) Antibody detects endogenous levels of PKC zeta only when phosphorylated at Threonine 410
Cross-Reactivity Human, Mouse (Murine), Rat (Rattus)
Purification The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.
Immunogen A synthesized peptide derived from human PKC zeta around the phosphorylation site of Threonine 410
Isotype IgG
Plasmids, Primers & others

Target Details PKC zeta

Product Details anti-PKC zeta Antibody Application Details Handling Images back to top
Antigen
Alternative Name PKC zeta (PRKCZ Antibody Abstract)
Background

Description: Calcium- and diacylglycerol-independent serine/threonine-protein kinase that functions in phosphatidylinositol 3-kinase (PI3K) pathway and mitogen-activated protein (MAP) kinase cascade, and is involved in NF-kappa-B activation, mitogenic signaling, cell proliferation, cell polarity, inflammatory response and maintenance of long-term potentiation (LTP). Upon lipopolysaccharide (LPS) treatment in macrophages, or following mitogenic stimuli, functions downstream of PI3K to activate MAP2K1/MEK1-MAPK1/ERK2 signaling cascade independently of RAF1 activation. Required for insulin-dependent activation of AKT3, but may function as an adapter rather than a direct activator. Upon insulin treatment may act as a downstream effector of PI3K and contribute to the activation of translocation of the glucose transporter SLC2A4/GLUT4 and subsequent glucose transport in adipocytes. In EGF-induced cells, binds and activates MAP2K5/MEK5-MAPK7/ERK5 independently of its kinase activity and can activate JUN promoter through MEF2C. Through binding with SQSTM1/p62, functions in interleukin-1 signaling and activation of NF-kappa-B with the specific adapters RIPK1 and TRAF6. Participates in TNF-dependent transactivation of NF-kappa-B by phosphorylating and activating IKBKB kinase, which in turn leads to the degradation of NF-kappa-B inhibitors. In migrating astrocytes, forms a cytoplasmic complex with PARD6A and is recruited by CDC42 to function in the establishment of cell polarity along with the microtubule motor and dynein. In association with FEZ1, stimulates neuronal differentiation in PC12 cells. In the inflammatory response, is required for the T-helper 2 (Th2) differentiation process, including interleukin production, efficient activation of JAK1 and the subsequent phosphorylation and nuclear translocation of STAT6. May be involved in development of allergic airway inflammation (asthma), a process dependent on Th2 immune response. In the NF-kappa-B-mediated inflammatory response, can relieve SETD6-dependent repression of NF-kappa-B target genes by phosphorylating the RELA subunit at 'Ser-311'. Necessary and sufficient for LTP maintenance in hippocampal CA1 pyramidal cells. In vein endothelial cells treated with the oxidant peroxynitrite, phosphorylates STK11 leading to nuclear export of STK11, subsequent inhibition of PI3K/Akt signaling, and increased apoptosis. Phosphorylates VAMP2 in vitro (PubMed:17313651).

Gene: PRKCZ

Molecular Weight 70kDa
Gene ID 5590
UniProt Q05513
Pathways NF-kappaB Signaling, RTK Signaling, Myometrial Relaxation and Contraction, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Synaptic Membrane, Production of Molecular Mediator of Immune Response, CXCR4-mediated Signaling Events, Thromboxane A2 Receptor Signaling

Application Details

Product Details anti-PKC zeta Antibody Target Details PKC zeta Handling Images back to top
Application Notes WB 1:500-1:2000 IHC 1:50-1:200 IF/ICC 1:100-1:500
Restrictions For Research Use only

Handling

Product Details anti-PKC zeta Antibody Target Details PKC zeta Application Details Images back to top
Format Liquid
Concentration 1 mg/mL
Buffer Rabbit IgG in phosphate buffered saline ,  pH 7.4, 150  mM NaCl, 0.02 % sodium azide and 50 % glycerol.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store at -20 °C.Stable for 12 months from date of receipt
Expiry Date 12 months

Images

Product Details anti-PKC zeta Antibody Target Details PKC zeta Application Details Handling back to top
Supplier Images
Immunohistochemistry (IHC) image for anti-Protein Kinase C, zeta (PRKCZ) (pThr410) antibody (ABIN6256558) ABIN6267613 at 1/100 staining human brain tissue sections by IHC-P. The tissue was fo...
Immunofluorescence (fixed cells) (IF/ICC) image for anti-Protein Kinase C, zeta (PRKCZ) (pThr410) antibody (ABIN6256558) ABIN6267613 staining NIH-3T3 by IF/ICC. The sample were fixed with PFA and permeabili...
Immunofluorescence (fixed cells) (IF/ICC) image for anti-Protein Kinase C, zeta (PRKCZ) (pThr410) antibody (ABIN6256558) ABIN6267613 staining C6 cells treated with λ phosphatase by ICC/IF. Cells were fixed ...
Western Blotting (WB) image for anti-Protein Kinase C, zeta (PRKCZ) (pThr410) antibody (ABIN6256558) Western blot analysis of PKC zeta phosphorylation expression in mouse liver lysates,T...
Western Blotting (WB) image for anti-Protein Kinase C, zeta (PRKCZ) (pThr410) antibody (ABIN6256558) Western blot analysis of PKC zeta phosphorylation expression in PMA treated NIH-3T3 w...