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|Antigen||Nuclear Receptor Subfamily 0, Group B, Member 2 (NR0B2) Antibodies|
|Epitope||AA 110-159 Alternatives|
|Reactivity||Dog (Canine), Human, Monkey, Rabbit Alternatives|
|Conjugate||This NR0B2 antibody is un-conjugated Alternatives|
Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
Product Details anti-NR0B2 AntibodyTarget Details NR0B2 Application Details Handling Images
|Predicted Reactivity||Percent identity by BLAST analysis: Human, Dog (100%) Horse (91%).|
Synthetic peptide located between aa110-159 of human NR0B2 (Q15466, NP_068804). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Tamarin, Dog, Rabbit (100%), Baboon, Galago (92%), Horse (91%), Elephant, Guinea pig (85%).
Type of Immunogen: Synthetic peptide
|Plasmids, Primers & others|
Target Details NR0B2Product Details anti-NR0B2 Antibody Application Details Handling Images back to top
|Alternative Name||NR0B2 / SHP (NR0B2 Antibody Abstract)|
Name/Gene ID: NR0B2
Subfamily: NR0 Knirps-like
Synonyms: NR0B2, Short heterodimer partner, SHP, Nuclear receptor SHP, Orphan nuclear receptor SHP, Small heterodimer partner, SHP1
|Pathways||Nuclear Receptor Transcription Pathway, Positive Regulation of Peptide Hormone Secretion, Intracellular Steroid Hormone Receptor Signaling Pathway, Steroid Hormone Mediated Signaling Pathway|
Application DetailsProduct Details anti-NR0B2 Antibody Target Details NR0B2 Handling Images back to top
Approved: IHC, IHC-P (5 μg/mL), WB
Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. ELISA titer using peptide based assay: 1:312500. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.
Target Species of Antibody: Human
|Restrictions||For Research Use only|
HandlingProduct Details anti-NR0B2 Antibody Target Details NR0B2 Application Details Images back to top
|Buffer||Lyophilized from PBS with 2 % sucrose|
|Handling Advice||Avoid repeat freeze-thaw cycles.|
|Storage||4 °C,-20 °C|
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
ImagesProduct Details anti-NR0B2 Antibody Target Details NR0B2 Application Details Handling back to top