anti-Dog (Canine) NR0B2 antibody for Western Blotting

Recommended NR0B2 Antibody

Antigen
Nuclear Receptor Subfamily 0, Group B, Member 2 (NR0B2) Antibodies
  • SHP
  • SHP1
  • SHP-1
  • Shp1
  • Shp
  • NR0B2-A
  • Nr0b2
  • SHP-A
  • gb:bc058069
  • nuclear receptor subfamily 0 group B member 2
  • nuclear receptor subfamily 0, group B, member 2
  • nuclear receptor subfamily 0, group B, member 2a
  • NR0B2
  • Nr0b2
  • nr0b2a
Epitope
AA 110-159
18
11
9
7
4
3
2
2
2
1
1
1
1
1
1
1
1
Reactivity
Dog (Canine), Human, Monkey, Rabbit
88
20
19
6
5
5
3
2
2
2
1
1
Host
Rabbit
70
16
4
Clonality
Polyclonal
Conjugate
This NR0B2 antibody is un-conjugated
4
3
3
2
2
2
1
1
1
1
1
1
Application
Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
72
38
32
27
16
13
12
6
3
1
1
1
1
Options

Available images

Catalog No. ABIN6741607
$ 727.83
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Clonality References Details
7.890334 ABIN2782296 IHC WB Rabbit Middle Region Polyclonal 1
4.890334 ABIN2774985 WB Rabbit Middle Region Polyclonal 1
4.890334 ABIN2782295 WB Rabbit N-Term Polyclonal 1
4.890334 ABIN2774986 WB Rabbit C-Term Polyclonal 1
4 ABIN6737786 WB Rabbit IgG AA 146-195 Polyclonal 0

General

Antigen Nuclear Receptor Subfamily 0, Group B, Member 2 (NR0B2) Antibodies
Epitope AA 110-159
(18), (11), (9), (7), (4), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Dog (Canine), Human, Monkey, Rabbit
(88), (20), (19), (6), (5), (5), (3), (2), (2), (2), (1), (1)
Host Rabbit
(70), (16), (4)
Clonality
Polyclonal
Conjugate This NR0B2 antibody is un-conjugated
(4), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Application Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(72), (38), (32), (27), (16), (13), (12), (6), (3), (1), (1), (1), (1)
Supplier

Product Details anti-NR0B2 Antibody

Target Details NR0B2 Application Details Handling Images
Brand IHC-plus™
Specificity Human NR0B2
Predicted Reactivity Percent identity by BLAST analysis: Human, Dog (100%) Horse (91%).
Purification Immunoaffinity purified
Immunogen Synthetic peptide located between aa110-159 of human NR0B2 (Q15466, NP_068804). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Tamarin, Dog, Rabbit (100%), Baboon, Galago (92%), Horse (91%), Elephant, Guinea pig (85%).

Type of Immunogen: Synthetic peptide
Isotype IgG
Plasmids, Primers & others

Target Details NR0B2

Product Details anti-NR0B2 Antibody Application Details Handling Images back to top
Antigen
Alternative Name NR0B2 / SHP (NR0B2 Antibody Abstract)
Background Name/Gene ID: NR0B2
Subfamily: NR0 Knirps-like
Family: NHR

Synonyms: NR0B2, Short heterodimer partner, SHP, Nuclear receptor SHP, Orphan nuclear receptor SHP, Small heterodimer partner, SHP1
Gene ID 8431
NCBI Accession NP_068804
UniProt Q15466
Pathways Nuclear Receptor Transcription Pathway, Positive Regulation of Peptide Hormone Secretion, Intracellular Steroid Hormone Receptor Signaling Pathway, Steroid Hormone Mediated Signaling Pathway

Application Details

Product Details anti-NR0B2 Antibody Target Details NR0B2 Handling Images back to top
Application Notes Approved: IHC, IHC-P (5 μg/mL), WB

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. ELISA titer using peptide based assay: 1:312500. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.
Comment

Target Species of Antibody: Human

Restrictions For Research Use only

Handling

Product Details anti-NR0B2 Antibody Target Details NR0B2 Application Details Images back to top
Format Lyophilized
Reconstitution Distilled Water.
Concentration Lot specific
Buffer Lyophilized from PBS with 2 % sucrose
Handling Advice Avoid repeat freeze-thaw cycles.
Storage 4 °C,-20 °C
Storage Comment Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Images

Product Details anti-NR0B2 Antibody Target Details NR0B2 Application Details Handling back to top
Supplier Images
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-Nuclear Receptor Subfamily 0, Group B, Member 2 (NR0B2) (AA 110-159) antibody (ABIN6741607) Human Liver (formalin-fixed, paraffin-embedded) stained with NR0B2 antibody ABIN46224...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-Nuclear Receptor Subfamily 0, Group B, Member 2 (NR0B2) (AA 110-159) antibody (ABIN6741607) Human Prostate (formalin-fixed, paraffin-embedded) stained with NR0B2 antibody ABIN46...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-Nuclear Receptor Subfamily 0, Group B, Member 2 (NR0B2) (AA 110-159) antibody (ABIN6741607) Human Spleen (formalin-fixed, paraffin-embedded) stained with NR0B2 antibody ABIN4622...
Immunohistochemistry (IHC) image for anti-Nuclear Receptor Subfamily 0, Group B, Member 2 (NR0B2) (AA 110-159) antibody (ABIN6741607) Anti-NR0B2 antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraf...