anti-Mouse (Murine) NR0B2 antibody for Immunohistochemistry (Paraffin-embedded Sections)

Recommended NR0B2 Antibody (supplied by: Log in to see )

Antigen
Nuclear Receptor Subfamily 0, Group B, Member 2 (NR0B2) Antibodies
  • SHP
  • SHP1
  • SHP-1
  • Shp1
  • Shp
  • NR0B2-A
  • Nr0b2
  • SHP-A
  • gb:bc058069
  • nuclear receptor subfamily 0 group B member 2
  • nuclear receptor subfamily 0, group B, member 2
  • nuclear receptor subfamily 0, group B, member 2a
  • NR0B2
  • Nr0b2
  • nr0b2a
Epitope
Ligand Binding Domain
19
11
10
7
4
3
2
2
1
1
1
1
1
1
1
1
1
1
Reactivity
Hamster, Human, Monkey, Mouse (Murine), Rabbit
82
13
11
6
6
5
3
2
2
2
1
Host
Rabbit
64
16
4
Clonality
Polyclonal
Conjugate
This NR0B2 antibody is un-conjugated
3
3
3
2
2
2
Application
Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
74
38
32
27
17
13
12
6
3
1
1
1
1
Options
Supplier
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Available images

Catalog No. ABIN1049128
$ 484.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN1387415 IF (p) IHC (p) WB Rabbit IgG AA 45-75 Log in to see Polyclonal 0
0.9458804 ABIN271164 IHC (p) Rabbit Log in to see Polyclonal 2

General

Antigen Nuclear Receptor Subfamily 0, Group B, Member 2 (NR0B2) Antibodies
Epitope Ligand Binding Domain
(19), (11), (10), (7), (4), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Hamster, Human, Monkey, Mouse (Murine), Rabbit
(82), (13), (11), (6), (6), (5), (3), (2), (2), (2), (1)
Host Rabbit
(64), (16), (4)
Clonality
Polyclonal
Conjugate This NR0B2 antibody is un-conjugated
(3), (3), (3), (2), (2), (2)
Application Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
(74), (38), (32), (27), (17), (13), (12), (6), (3), (1), (1), (1), (1)
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Product Details anti-NR0B2 Antibody

Target Details NR0B2 Application Details Handling Images
Brand IHC-plus™
Specificity Human NR0B2. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
Predicted Reactivity Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Hamster, Rabbit (100%) Rat, Bovine, Bat (94%) Elephant, Panda, Horse, Pig, Opossum (88%) Dog (81%).
Purification Immunoaffinity purified
Immunogen Synthetic 16 amino acid peptide from ligand-binding domain of human NR0B2. Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Hamster, Rabbit (100%), Rat, Bovine, Bat (94%), Elephant, Panda, Horse, Pig, Opossum (88%), Dog (81%).

Type of Immunogen: Synthetic peptide
Plasmids, Primers & others

Target Details NR0B2

Product Details anti-NR0B2 Antibody Application Details Handling Images back to top
Antigen
Alternative Name NR0B2 / SHP (NR0B2 Antibody Abstract)
Background Name/Gene ID: NR0B2
Subfamily: NR0 Knirps-like
Family: NHR

Synonyms: NR0B2, Short heterodimer partner, SHP, Nuclear receptor SHP, Orphan nuclear receptor SHP, Small heterodimer partner, SHP1
Gene ID 8431
Pathways Nuclear Receptor Transcription Pathway, Positive Regulation of Peptide Hormone Secretion, Intracellular Steroid Hormone Receptor Signaling Pathway, Steroid Hormone Mediated Signaling Pathway

Application Details

Product Details anti-NR0B2 Antibody Target Details NR0B2 Handling Images back to top
Application Notes Approved: ICC, IF, IHC, IHC-P (4 μg/mL)
Comment

Target Species of Antibody: Human

Assay Procedure The IHC-pro Immunohistochemistry Protocol

Tissue Preparation
Formalin fixation and embedding in paraffin wax

Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.

Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.

Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.

Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.

Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip
Restrictions For Research Use only

Handling

Product Details anti-NR0B2 Antibody Target Details NR0B2 Application Details Images back to top
Format Liquid
Concentration Lot specific
Buffer PBS, less than 0.1 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C,-20 °C
Storage Comment Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
Expiry Date 12 months

Images

Product Details anti-NR0B2 Antibody Target Details NR0B2 Application Details Handling back to top
Supplier Images
Immunohistochemistry (IHC) image for anti-Nuclear Receptor Subfamily 0, Group B, Member 2 (NR0B2) (Ligand Binding Domain) antibody (ABIN1049128) Anti-NR0B2 antibody ABIN1049128 IHC staining of human liver, hepatocytes. Immunohist...
Immunohistochemistry (IHC) image for anti-Nuclear Receptor Subfamily 0, Group B, Member 2 (NR0B2) (Ligand Binding Domain) antibody (ABIN1049128) Anti-NR0B2 antibody IHC of human liver, hepatocytes. Immunohistochemistry of formalin...