anti-Zebrafish (Danio rerio) NR0B2 antibody for Western Blotting

Recommended NR0B2 Antibody

Antigen
Nuclear Receptor Subfamily 0, Group B, Member 2 (NR0B2) Antibodies
  • SHP
  • SHP1
  • SHP-1
  • Shp1
  • Shp
  • NR0B2-A
  • Nr0b2
  • SHP-A
  • gb:bc058069
  • nuclear receptor subfamily 0 group B member 2
  • nuclear receptor subfamily 0, group B, member 2
  • nuclear receptor subfamily 0, group B, member 2a
  • NR0B2
  • Nr0b2
  • nr0b2a
Epitope
C-Term
18
11
9
7
4
3
2
2
2
1
1
1
1
1
1
1
1
Reactivity
Cow (Bovine), Dog (Canine), Guinea Pig, Horse (Equine), Human, Rabbit, Rat (Rattus), Zebrafish (Danio rerio)
88
19
19
5
5
5
3
2
2
1
1
Host
Rabbit
70
16
4
Clonality
Polyclonal
Conjugate
This NR0B2 antibody is un-conjugated
4
3
3
2
2
2
1
1
1
1
1
1
Application
Western Blotting (WB)
72
39
32
27
16
13
13
6
3
1
1
1
1
Options

Available images

Catalog No. ABIN2774986
$ 289.00
Plus shipping costs $45.00

General

Antigen Nuclear Receptor Subfamily 0, Group B, Member 2 (NR0B2) Antibodies
Epitope C-Term
(18), (11), (9), (7), (4), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Cow (Bovine), Dog (Canine), Guinea Pig, Horse (Equine), Human, Rabbit, Rat (Rattus), Zebrafish (Danio rerio)
(88), (19), (19), (5), (5), (5), (3), (2), (2), (1), (1)
Host Rabbit
(70), (16), (4)
Clonality
Polyclonal
Conjugate This NR0B2 antibody is un-conjugated
(4), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Application Western Blotting (WB)
(72), (39), (32), (27), (16), (13), (13), (6), (3), (1), (1), (1), (1)
Pubmed 1 reference available
Supplier

Product Details anti-NR0B2 Antibody

Target Details NR0B2 Application Details Handling References for anti-NR0B2 antibody (ABIN2774986) Images
Blocking Peptide Blocking peptide for this product available: ABIN980868
Sequence LQAASHIGHL QQEAHWVLCE VLEPWCPAAQ GRLTRVLLTA STLKSIPTSL
Predicted Reactivity Cow: 93%, Dog: 93%, Guinea Pig: 93%, Horse: 93%, Human: 100%, Rabbit: 93%, Rat: 93%, Zebrafish: 92%
Characteristics This is a rabbit polyclonal antibody against NR0B2. It was validated on Western Blot using a cell lysate as a positive control.
Purification Affinity Purified
Immunogen The immunogen is a synthetic peptide directed towards the C terminal region of human NR0B2
Plasmids, Primers & others

Target Details NR0B2

Product Details anti-NR0B2 Antibody Application Details Handling References for anti-NR0B2 antibody (ABIN2774986) Images back to top
Antigen
Alternative Name NR0B2 (NR0B2 Antibody Abstract)
Background NR0B2 is an unusual orphan receptor that contains a putative ligand-binding domain but lacks a conventional DNA-binding domain. It is a member of the nuclear hormone receptor family, a group of transcription factors regulated by small hydrophobic hormones, a subset of which do not have known ligands and are referred to as orphan nuclear receptors. The protein has been shown to interact with retinoid and thyroid hormone receptors, inhibiting their ligand-dependent transcriptional activation. In addition, interaction with estrogen receptors has been demonstrated, leading to inhibition of function. Studies suggest that the protein represses nuclear hormone receptor-mediated transactivation via two separate steps: competition with coactivators and the direct effects of its transcriptional repressor function. The protein encoded by this gene is an unusual orphan receptor that contains a putative ligand-binding domain but lacks a conventional DNA-binding domain. The gene product is a member of the nuclear hormone receptor family, a group of transcription factors regulated by small hydrophobic hormones, a subset of which do not have known ligands and are referred to as orphan nuclear receptors. The protein has been shown to interact with retinoid and thyroid hormone receptors, inhibiting their ligand-dependent transcriptional activation. In addition, interaction with estrogen receptors has been demonstrated, leading to inhibition of function. Studies suggest that the protein represses nuclear hormone receptor-mediated transactivation via two separate steps: competition with coactivators and the direct effects of its transcriptional repressor function. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.
Alias Symbols: SHP, SHP1
Protein Interaction Partner: TP53, SMAD4, IL3RA, HNRNPA1, FN1, PEF1, RBP5, ZMYND10, CHRD, ZAP70, RXRA, PLSCR1, Nr1i3, EID1, NR5A2, ESRRG, MDM2, RNF31, UBC, HDAC3, HDAC1, GPS2, SIN3A, EHMT2, TRAF6, SMARCA2, SNW1, VDR, RARG, PPARD, HNF4G, HNF4A, ESRRA, RUNX2, Prmt5, SIRT6, SIRT7, SIRT1
Protein Size: 257
Molecular Weight 28 kDa
Gene ID 8431
NCBI Accession NM_021969, NP_068804
UniProt Q15466
Pathways Nuclear Receptor Transcription Pathway, Positive Regulation of Peptide Hormone Secretion, Intracellular Steroid Hormone Receptor Signaling Pathway, Steroid Hormone Mediated Signaling Pathway

Application Details

Product Details anti-NR0B2 Antibody Target Details NR0B2 Handling References for anti-NR0B2 antibody (ABIN2774986) Images back to top
Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 257 AA

Restrictions For Research Use only

Handling

Product Details anti-NR0B2 Antibody Target Details NR0B2 Application Details References for anti-NR0B2 antibody (ABIN2774986) Images back to top
Format Liquid
Concentration Lot specific
Buffer Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-NR0B2 antibody (ABIN2774986)

Product Details anti-NR0B2 Antibody Target Details NR0B2 Application Details Handling Images back to top
Background publications

Park, Park, An, Choi, Kim, Cheong, Yang: "Bile acid regulates c-Jun expression through the orphan nuclear receptor SHP induction in gastric cells." in: Biochemical and biophysical research communications, Vol. 369, Issue 2, pp. 437-43, 2008

Images

Product Details anti-NR0B2 Antibody Target Details NR0B2 Application Details Handling References for anti-NR0B2 antibody (ABIN2774986) back to top
Supplier Images
Western Blotting (WB) image for anti-Nuclear Receptor Subfamily 0, Group B, Member 2 (NR0B2) (C-Term) antibody (ABIN2774986) WB Suggested Anti-NR0B2 Antibody Titration: 0.2-1 ug/ml ELISA Titer: 1:62500 Posi...