anti-Dog (Canine) Nuclear Receptor Subfamily 1, Group D, Member 2 antibody for Immunohistochemistry

Recommended Nuclear Receptor Subfamily 1, Group D, Member 2 Antibody (supplied by: Log in to see )

Antigen
Nuclear Receptor Subfamily 1, Group D, Member 2 (NR1D2) Antibodies
  • BD73
  • EAR-1R
  • RVR
  • HZF-2
  • Rev-erb
  • REV-ERBB
  • NR1D2
  • rvr
  • bd73
  • hzf2
  • ear-1r
  • hs.37288
  • NR1D2-A
  • Reverbbeta-A
  • reverbb1
  • nuclear receptor subfamily 1 group D member 2
  • nuclear receptor subfamily 1, group D, member 2
  • nuclear receptor subfamily 1, group D, member 2a
  • NR1D2
  • Nr1d2
  • nr1d2
  • nr1d2a
Epitope
Ligand Binding Domain
11
11
10
9
8
7
5
5
4
4
3
2
2
1
1
Reactivity
Bat, Cow (Bovine), Dog (Canine), Horse (Equine), Human, Monkey, Rabbit
77
17
14
9
9
9
8
6
6
5
5
3
2
2
1
1
1
1
Host
Rabbit
65
15
Clonality
Polyclonal
Conjugate
Un-conjugated
3
3
3
2
2
2
Application
Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
55
29
29
22
5
3
3
2
1
1
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Catalog No. ABIN1049132
$ 484.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
8.404079 ABIN410772 ELISA IHC IHC (p) Rabbit Internal Region Log in to see Polyclonal 0
8.404079 ABIN2470775 IHC ELISA Rabbit C-Term Log in to see Polyclonal 0

General

Antigen Nuclear Receptor Subfamily 1, Group D, Member 2 (NR1D2) Antibodies
Epitope Ligand Binding Domain
(11), (11), (10), (9), (8), (7), (5), (5), (4), (4), (3), (2), (2), (1), (1)
Reactivity Bat, Cow (Bovine), Dog (Canine), Horse (Equine), Human, Monkey, Rabbit
(77), (17), (14), (9), (9), (9), (8), (6), (6), (5), (5), (3), (2), (2), (1), (1), (1), (1)
Host Rabbit
(65), (15)
Clonality
Polyclonal
Conjugate Un-conjugated
(3), (3), (3), (2), (2), (2)
Application Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
(55), (29), (29), (22), (5), (3), (3), (2), (1), (1)
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Product details

Target Details Application Details Handling Images
Brand IHC-plus™
Specificity Human NR1D2. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
Predicted Reactivity Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Bovine, Dog, Bat, Panda, Horse, Rabbit, Opossum, Platypus (100%) Mouse, Rat, Elephant (94%) Chicken (82%).
Purification Immunoaffinity purified
Immunogen Synthetic 17 amino acid peptide from ligand-binding domain of human NR1D2. Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Bovine, Dog, Bat, Panda, Horse, Rabbit, Opossum, Platypus (100%), Mouse, Rat, Elephant (94%), Chicken (82%).

Type of Immunogen: Synthetic peptide

Target Details

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Antigen
Alternative Name NR1D2 (NR1D2 Antibody Abstract)
Background Name/Gene ID: NR1D2
Subfamily: NR1 Thyroid hormone-like
Family: NHR

Synonyms: NR1D2, EAR-1R, HZF2, Nuclear receptor rvr, Rev-erbabeta, Rev-erb-beta, Rev-erba beta, RVR, BD73, Nuclear receptor ear-1r
Gene ID 9975
Pathways Nuclear Receptor Transcription Pathway, Steroid Hormone Mediated Signaling Pathway

Application Details

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Application Notes Approved: IHC, IHC-P (4 - 7 μg/mL)
Comment

Target Species of Antibody: Human

Assay Procedure The IHC-pro Immunohistochemistry Protocol

Tissue Preparation
Formalin fixation and embedding in paraffin wax

Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.

Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.

Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.

Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.

Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip
Restrictions For Research Use only

Handling

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Format Liquid
Concentration Lot specific
Buffer PBS, less than 0.1 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C,-20 °C
Storage Comment Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
Expiry Date 12 months

Images

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Supplier Images
Immunohistochemistry (IHC) image for anti-Nuclear Receptor Subfamily 1, Group D, Member 2 (NR1D2) (Ligand Binding Domain) antibody (ABIN1049132) Anti-NR1D2 antibody ABIN1049132 IHC staining of human brain, cerebellum. Immunohisto...
Immunohistochemistry (IHC) image for anti-Nuclear Receptor Subfamily 1, Group D, Member 2 (NR1D2) (Ligand Binding Domain) antibody (ABIN1049132) Anti-NR1D2 antibody IHC of human Skin, Melanoma. Immunohistochemistry of formalin-fix...