anti-Human BAX antibody for Flow Cytometry

Recommended BAX Antibody (supplied by: Log in to see )

Antigen
BCL2-Associated X Protein (BAX) Antibodies
  • BAX-ALPHA
  • bax-A
  • xBax
  • xlbax
  • BAX
  • bax
  • fj16e01
  • wu:fc50b10
  • wu:fj16e01
  • BCL2L4
  • BCL2 associated X, apoptosis regulator
  • BCL2-associated X protein L homeolog
  • BCL2-associated X protein
  • bcl2-associated X protein, a
  • BAX
  • bax.L
  • bax
  • baxa
  • Bax
Epitope
AA 41-76, BH3 Domain
88
77
31
22
21
19
15
13
12
12
10
10
9
9
8
7
7
5
4
4
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
555
233
213
128
44
31
28
23
21
11
7
7
6
5
3
3
3
2
2
2
1
1
1
1
1
Host
Rabbit
346
243
4
2
Clonality
Polyclonal
Conjugate
This BAX antibody is un-conjugated
19
15
14
12
11
9
8
6
5
4
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
Application
Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
453
230
194
168
142
139
113
47
42
40
32
26
13
5
5
2
2
1
1
1
1
1
1
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Catalog No. ABIN388094
$ 335.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
14.319576 ABIN725390 FACS IF (cc) IF (p) IHC (p) WB Rabbit IgG AA 60-110 Log in to see Polyclonal 24
14.319576 ABIN5949413 FACS IHC IHC (p) IP WB Rabbit IgG AA 20-150 Log in to see SZ3-07 0
14.319576 ABIN2657050 FACS Alexa Fluor 488 Mouse IgG1 kappa Log in to see 2D2 7
14.319576 ABIN3030145 FACS IHC ELISA WB Rabbit Ig Fraction AA 41-76 Log in to see Polyclonal 0
14.319576 ABIN5774249 FACS FITC Rabbit IgG Log in to see T22-A 0
1 ABIN4283106 FACS ICC IF IHC IHC (fro) IHC (p) WB Mouse IgG1 kappa AA 3-16 Log in to see 2D2 1
1 ABIN451743 ELISA FACS ICC IF IHC IHC (fro) IP WB Mouse IgG1 kappa N-Term Log in to see 6A7 3
1 ABIN2664009 FACS Rabbit IgG Log in to see Polyclonal 9
1 ABIN2736349 FACS IF IP IHC WB Rabbit IgG Log in to see Polyclonal 0
1 ABIN4903010 FACS IF IHC WB Mouse IgG Log in to see 0
1 ABIN2208719 FACS IF IP IHC ELISA WB Mouse IgG1 AA 3-16 Log in to see 2D2 0
1 ABIN6175302 FACS IF IHC (f) WB CF®770 Mouse IgG1 AA 3-16 Log in to see 2D2 0
1 ABIN6175304 FACS IF IHC (f) WB CF®680 Mouse IgG1 AA 3-16 Log in to see 2D2 0
1 ABIN6175306 FACS IF IHC (f) WB CF®680R Mouse IgG1 AA 3-16 Log in to see 2D2 0
1 ABIN6175286 FACS IF IHC (f) WB CF®405S Mouse IgG1 AA 3-16 Log in to see 2D2 0
1 ABIN6175290 FACS IF IHC (f) WB CF®640R Mouse IgG1 AA 3-16 Log in to see 2D2 0
1 ABIN6175294 FACS IF IHC (f) WB CF®647 Mouse IgG1 AA 3-16 Log in to see 2D2 0
1 ABIN6175292 FACS IF IHC (f) WB CF®543 Mouse IgG1 AA 3-16 Log in to see 2D2 0
1 ABIN6175298 FACS IF IHC (f) WB CF®660R Mouse IgG1 AA 3-16 Log in to see 2D2 0
1 ABIN6175310 FACS IF IHC (f) WB CF®594 Mouse IgG1 AA 3-16 Log in to see 2D2 0

General

Antigen BCL2-Associated X Protein (BAX) Antibodies
Epitope AA 41-76, BH3 Domain
(88), (77), (31), (22), (21), (19), (15), (13), (12), (12), (10), (10), (9), (9), (8), (7), (7), (5), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(555), (233), (213), (128), (44), (31), (28), (23), (21), (11), (7), (7), (6), (5), (3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1)
Host Rabbit
(346), (243), (4), (2)
Clonality (Clone)
Polyclonal   ( )
Conjugate This BAX antibody is un-conjugated
(19), (15), (14), (12), (11), (9), (8), (6), (5), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2)
Application Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(453), (230), (194), (168), (142), (139), (113), (47), (42), (40), (32), (26), (13), (5), (5), (2), (2), (1), (1), (1), (1), (1), (1)
Pubmed 13 references available
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Product Details anti-BAX Antibody

Target Details BAX Application Details Handling References for anti-BAX antibody (ABIN388094) Images
Specificity This Bax antibody was developed against a synthetic peptide between 51~81 amino acids from the N-terminal region (BH3 domain) of human Bax.
Predicted Reactivity Cow (Bovine)
Purification This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Immunogen This Bax antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 41-76 AA from human Bax.
Clone RB0952
Isotype Ig Fraction
Plasmids, Primers & others

Target Details BAX

Product Details anti-BAX Antibody Application Details Handling References for anti-BAX antibody (ABIN388094) Images back to top
Antigen
Alternative Name Bax (BAX Antibody Abstract)
Background Bax belongs to the BCL2 protein family. BCL2 family members form hetero- or homodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. Bax forms a heterodimer with BCL2, and functions as an apoptotic activator. This protein is reported to interact with, and increase the opening of, the mitochondrial voltage-dependent anion channel (VDAC), which leads to the loss in membrane potential and the release of cytochrome c. The expression of the Bax gene is regulated by the tumor suppressor P53 and has been shown to be involved in P53-mediated apoptosis.
Synonyms: apoptosis regulator BAX
Molecular Weight 24220 DA
Gene ID 581
UniProt Q07814
Pathways p53 Signaling, PI3K-Akt Signaling, Apoptosis, Caspase Cascade in Apoptosis, Positive Regulation of Endopeptidase Activity, Unfolded protein response

Application Details

Product Details anti-BAX Antibody Target Details BAX Handling References for anti-BAX antibody (ABIN388094) Images back to top
Application Notes WB = 1:1000, IHC (p) = 1:50-100, FACS = 1:10-50
Restrictions For Research Use only

Handling

Product Details anti-BAX Antibody Target Details BAX Application Details References for anti-BAX antibody (ABIN388094) Images back to top
Format Liquid
Concentration 1.6 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Expiry Date 6 months

References for anti-BAX antibody (ABIN388094)

Product Details anti-BAX Antibody Target Details BAX Application Details Handling Images back to top
Product cited in:

Cui, Lin, Shi, Pan: "Hypoxic postconditioning attenuates apoptosis via inactivation of adenosine A2a receptor through NDRG3-Raf-ERK pathway." in: Biochemical and biophysical research communications, Vol. 491, Issue 2, pp. 277-284, 2017 Method employed by authors: Western Blotting (WB)

Lin, Ma, Wang, Zhao, Liu, Pu, Hu, Hu, Shao: "Edaravone ameliorates compression-induced damage in rat nucleus pulposus cells." in: Life sciences, Vol. 189, pp. 76-83, 2017 Method employed by authors: Western Blotting (WB)

Hu, Kuo, Senadi, Chang, Wang: "Photodynamic Therapy Using Indolines-Fused-Triazoles Induces Mitochondrial Apoptosis in Human Non-Melanoma BCC Cells." in: Anticancer research, Vol. 37, Issue 10, pp. 5499-5505, 2017 Method employed by authors: Immunofluorescence (IF)

Wang, Wang, Li, Yang, Xing, Chen, Xu: "HMGB1 knockdown effectively inhibits the progression of rectal cancer by suppressing HMGB1 expression and promoting apoptosis of rectal cancer cells." in: Molecular medicine reports, Vol. 14, Issue 1, pp. 1026-32, 2016 Method employed by authors: Western Blotting (WB)

Soni, Adebiyi: "Early septic insult in neonatal pigs increases serum and urinary soluble Fas ligand and decreases kidney function without inducing significant renal apoptosis." in: Renal failure, pp. 1-12, 2016 Method employed by authors: Western Blotting (WB)

Li, Wang, Wang, Gao, Zhang, Lu: "Oridonin phosphate-induced autophagy effectively enhances cell apoptosis of human breast cancer cells." in: Medical oncology (Northwood, London, England), Vol. 32, Issue 1, pp. 365, 2014 Method employed by authors: Immunofluorescence (IF)

Gavathiotis, Reyna, Davis, Bird, Walensky: "BH3-triggered structural reorganization drives the activation of proapoptotic BAX." in: Molecular cell, Vol. 40, Issue 3, pp. 481-92, 2010 Method employed by authors: Functional Studies (Func)

Granado, Gangoiti, Ouro, Arana, Gómez-Muñoz: "Ceramide 1-phosphate inhibits serine palmitoyltransferase and blocks apoptosis in alveolar macrophages." in: Biochimica et biophysica acta, Vol. 1791, Issue 4, pp. 263-72, 2009 Method employed by authors: Western Blotting (WB)

Background publications

Liu, Newland, Jia: "Bax conformational change is a crucial step for PUMA-mediated apoptosis in human leukemia." in: Biochemical and biophysical research communications, Vol. 310, Issue 3, pp. 956-62, 2003

Roucou, Guo, Zhang, Goodyer, LeBlanc: "Cytosolic prion protein is not toxic and protects against Bax-mediated cell death in human primary neurons." in: The Journal of biological chemistry, Vol. 278, Issue 42, pp. 40877-81, 2003

Cao, Deng, May: "Cleavage of Bax to p18 Bax accelerates stress-induced apoptosis, and a cathepsin-like protease may rapidly degrade p18 Bax." in: Blood, Vol. 102, Issue 7, pp. 2605-14, 2003

McJilton, Van Sikes, Wescott, Wu, Foreman, Gregory, Weidner, Harris Ford, Morgan Lasater, Mohler, Terrian: "Protein kinase Cepsilon interacts with Bax and promotes survival of human prostate cancer cells." in: Oncogene, Vol. 22, Issue 39, pp. 7958-68, 2003

Bidère, Lorenzo, Carmona, Laforge, Harper, Dumont, Senik: "Cathepsin D triggers Bax activation, resulting in selective apoptosis-inducing factor (AIF) relocation in T lymphocytes entering the early commitment phase to apoptosis." in: The Journal of biological chemistry, Vol. 278, Issue 33, pp. 31401-11, 2003

Images

Product Details anti-BAX Antibody Target Details BAX Application Details Handling References for anti-BAX antibody (ABIN388094) back to top
Supplier Images
Western Blotting (WB) image for anti-BCL2-Associated X Protein (BAX) (AA 41-76), (BH3 Domain) antibody (ABIN388094) Bax Antibody BH3(ABIN388094) western blot analysis in HL-60 cell line lysates (35 µg/...
Immunohistochemistry (IHC) image for anti-BCL2-Associated X Protein (BAX) (AA 41-76), (BH3 Domain) antibody (ABIN388094) Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with Bax Antibody ...
Immunohistochemistry (IHC) image for anti-BCL2-Associated X Protein (BAX) (AA 41-76), (BH3 Domain) antibody (ABIN388094) Flow cytometric analysis of HepG2 cells using Bax Antibody (BH3 Domain Specific)(bott...
Flow Cytometry (FACS) image for anti-BCL2-Associated X Protein (BAX) (AA 41-76), (BH3 Domain) antibody (ABIN388094) Overlay histogram showing HeLa cells stained with ABIN2837860(green line). The cells ...
Western Blotting (WB) image for anti-BCL2-Associated X Protein (BAX) (AA 41-76), (BH3 Domain) antibody (ABIN388094) Anti-Bax Antibody (BH3) at 1:2000 dilution + mouse spleen lysate Lysates/proteins at ...
Western Blotting (WB) image for anti-BCL2-Associated X Protein (BAX) (AA 41-76), (BH3 Domain) antibody (ABIN388094) All lanes : Anti-Bax Antibody (BH3) at 1:2000 dilution Lane 1: HT-1080 whole cell lys...
Western Blotting (WB) image for anti-BCL2-Associated X Protein (BAX) (AA 41-76), (BH3 Domain) antibody (ABIN388094) All lanes : Anti-Bax Antibody (BH3 Domain Specific) at 1:2000 dilution Lane 1: HT-108...