|Antigen||Protein Phosphatase 1, Regulatory Subunit 13B (PPP1R13B) Antibodies|
|Epitope||Internal Region Alternatives|
|Reactivity||Human, Mouse (Murine) Alternatives|
|Conjugate||This PPP1R13B antibody is un-conjugated Alternatives|
Enzyme Immunoassay (EIA), Western Blotting (WB)
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Product Details anti-PPP1R13B AntibodyTarget Details PPP1R13B Application Details Handling Images
|Specificity||This antibody is directed against ASPP1.|
Species reactivity (expected):Mouse.
Species reactivity (tested):Human.
|Characteristics||Concentration: 1.3 mg/mL (by UV absorbance at 280 nm)|
|Immunogen||Synthetic peptide corresponding to an internal sequence of human ASPP1|
Target Details PPP1R13BProduct Details anti-PPP1R13B Antibody Application Details Handling Images back to top
|Alternative Name||PPP1R13B (PPP1R13B Antibody Abstract)|
|Background||ASPP (ankyrin-repeat-, SH3-domain- proline-rich-region protein) proteins (ASPP1, ASPP2 and iASPP) represent a new family of p53 binding proteins. ASPP1 and ASPP2 bind and enhance p53-mediated apoptosis. In contrast, iASPP functionally inactivates p53. ASPPs may also regulate p63- and p73-mediated apoptosis. Both ASPP1 and 2 directly interact with p53 and specifically enhance the apoptotic function of p53 by stimulating its DNA binding and transactivation function on promoters of pro-apoptotic genes, such as Bax and PIG-3. Not all cell cycle arrest genes are affected, such as p21. Interestingly, expression of ASPP is frequently down-regulated in human breast carcinomas expressing wild-type p53 but not mutant p53. Therefore, ASPP might regulate the tumor suppression function of p53 in vivo.Synonyms: ASPP1, Apoptosis-stimulating of p53 protein 1, KIAA0771, Protein phosphatase 1 regulatory subunit 13B|
Application DetailsProduct Details anti-PPP1R13B Antibody Target Details PPP1R13B Handling Images back to top
ELISA: 1/2,500 - 1/10,000. Western Blot: 1/500 - 1/3,000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-PPP1R13B Antibody Target Details PPP1R13B Application Details Images back to top
|Buffer||0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing 0.01 % (w/v) Sodium Azide|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid repeated freezing and thawing.|