Myosin Phosphatase, Target Subunit 1 (PPP1R12A) (Middle Region) Peptide
PPP1R12A
Reactivity: Human
Host: Synthetic
BP, WB
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- Target See all PPP1R12A products
- PPP1R12A (Myosin Phosphatase, Target Subunit 1 (PPP1R12A))
- Protein Region
- Middle Region
- Origin
- Human
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Source
- Synthetic
- Application
- Blocking Peptide (BP), Western Blotting (WB)
- Sequence
- RRPREKRRST GVSFWTQDSD ENEQEQQSDT EEGSNKKETQ TDSISRYETS
- Characteristics
- This is a synthetic peptide designed for use in combination with anti- PPP1R12A Antibody. It may block above mentioned antibody from binding to its target protein in western blot and/or immunohistochecmistry under proper experimental settings. There is no guarantee for its use in other applications.
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Add 100 μL of sterile PBS. Final peptide concentration is 1 mg/mL in PBS.
- Storage
- -20 °C
- Storage Comment
- For longer periods of storage, store at -20°C. Avoid repeat freeze-thaw cycles.
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- Target
- PPP1R12A (Myosin Phosphatase, Target Subunit 1 (PPP1R12A))
- Synonyms
- 1200015F06Rik Peptide, 5730577I22Rik Peptide, AA792106 Peptide, AV099298 Peptide, D10Ertd625e Peptide, Mypt1 Peptide, M130 Peptide, MBS Peptide, MYPT1 Peptide, M110 Peptide, MBSP Peptide, protein phosphatase 1, regulatory (inhibitor) subunit 12A Peptide, protein phosphatase 1 regulatory subunit 12A Peptide, protein phosphatase 1, regulatory subunit 12A Peptide, Ppp1r12a Peptide, PPP1R12A Peptide
- Background
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Myosin phosphatase target subunit 1, which is also called the myosin-binding subunit of myosin phosphatase, is one of the subunits of myosin phosphatase. Myosin phosphatase regulates the interaction of actin and myosin downstream of the guanosine triphosphatase Rho. The small guanosine triphosphatase Rho is implicated in myosin light chain (MLC) phosphorylation, which results in contraction of smooth muscle and interaction of actin and myosin in nonmuscle cells. The guanosine triphosphate (GTP)-bound, active form of RhoA (GTP.RhoA) specifically interacted with the myosin-binding subunit (MBS) of myosin phosphatase, which regulates the extent of phosphorylation of MLC. Rho-associated kinase (Rho-kinase), which is activated by GTP. RhoA, phosphorylated MBS and consequently inactivated myosin phosphatase. Overexpression of RhoA or activated RhoA in NIH 3T3 cells increased phosphorylation of MBS and MLC. Thus, Rho appears to inhibit myosin phosphatase through the action of Rho-kinase. Several transcript variants encoding different isoforms have been found for this gene.
Alias Symbols: MBS, M130, MYPT1
Protein Size: 1030 - Gene ID
- 4659
- NCBI Accession
- NM_001143885, NP_001137357
- UniProt
- O14974
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