Polymerase (DNA Directed) iota (POLI) (N-Term) Peptide
POLI
Reactivity: Human
Host: Synthetic
BP, WB
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- Target See all POLI products
- POLI (Polymerase (DNA Directed) iota (POLI))
- Protein Region
- N-Term
- Origin
- Human
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Source
- Synthetic
- Application
- Blocking Peptide (BP), Western Blotting (WB)
- Sequence
- PVVERLGFDE NFVDLTEMVE KRLQQLQSDE LSAVTVSGHV YNNQSINLLD
- Characteristics
- This is a synthetic peptide designed for use in combination with anti-POLI Antibody(ARP52299_P050),. It may block above mentioned antibody from binding to its target protein in western blot and/or immunohistochecmistry under proper experimental settings. There is no guarantee for its use in other applications.
- Purification
- Purified
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- Application Notes
- Each Investigator should determine their own optimal working dilution for specific applications.
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Add 100 μL of sterile PBS. Final peptide concentration is 1 mg/mL in PBS.
- Concentration
- 1 mg/mL
- Buffer
- Final peptide concentration is 1 mg/mL in PBS.
- Handling Advice
- Avoid repeated freeze-thaw cycles.
- Storage
- -20 °C
- Storage Comment
- For longer periods of storage, store at -20°C. Avoid repeat freeze-thaw cycles.
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- Target
- POLI (Polymerase (DNA Directed) iota (POLI))
- Synonyms
- poli Peptide, wu:fc27h11 Peptide, zgc:110185 Peptide, GB12093 Peptide, NV16187 Peptide, RAD30B Peptide, RAD3OB Peptide, Rad30b Peptide, DNA polymerase iota Peptide, polymerase (DNA directed) iota Peptide, polymerase (DNA directed), iota Peptide, POLI Peptide, poli Peptide, LOC551219 Peptide, AFUA_4G11140 Peptide, LOC5568276 Peptide, Pol-iota Peptide, Poli Peptide
- Background
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POLI is an error-prone DNA polymerase specifically involved in DNA repair.POLI plays an important role in translesion synthesis, where the normal high-fidelity DNA polymerases cannot proceed and DNA synthesis stalls.POLI favors Hoogsteen base-pairing in the active site.POLI inserts the correct base with high-fidelity opposite an adenosine template.POLI exhibits low fidelity and efficiency opposite a thymidine template, where it will preferentially insert guanosine.POLI may play a role in hypermutation of immunogobulin genes.POLI forms a Schiff base with 5'-deoxyribose phosphate at abasic sites, but may not have lyase activity.
Alias Symbols: RAD30B, RAD3OB
Protein Interaction Partner: REV1,CALCOCO2,DAZAP2,DVL2,PCNA,POLH,REV1,RNF11,UBA52,UBB,CALCOCO2,DAZAP2,DVL2
Protein Size: 740 - Molecular Weight
- 83 kDa
- Gene ID
- 11201
- NCBI Accession
- NM_007195, NP_009126
- UniProt
- Q9UNA4
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