anti-Human RFWD2 antibody for Western Blotting

Recommended RFWD2 Antibody (supplied by: Log in to see )

Antigen
Ring Finger and WD Repeat Domain 2, E3 Ubiquitin Protein Ligase (RFWD2) Antibodies
  • COP1
  • RNF200
  • AI316802
  • C80879
  • Cop1
  • RGD1304773
  • RFWD2
  • cop1
  • rnf200
  • zgc:163067
  • ring finger and WD repeat domain 2
  • ring finger and WD repeat domain 2, E3 ubiquitin protein ligase
  • ring finger and WD repeat domain 2, E3 ubiquitin protein ligase L homeolog
  • RFWD2
  • Rfwd2
  • rfwd2
  • rfwd2.L
Epitope
AA 632-732
18
16
10
8
7
4
3
3
3
2
2
2
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine)
80
36
7
4
4
3
3
3
2
2
1
1
1
1
Host
Mouse
77
3
2
Clonality
Monoclonal
Conjugate
This RFWD2 antibody is un-conjugated
3
3
3
3
3
3
Application
Immunofluorescence (IF), Western Blotting (WB)
80
53
8
5
4
3
1
Options
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Available images

Catalog No. ABIN394529
$ 527.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
19.728132 ABIN566254 IF ELISA WB Mouse IgG2b kappa AA 632-731 Log in to see 1E4 0
15.75221 ABIN3184044 ELISA WB Rabbit IgG Tyr762 Log in to see Polyclonal 0
15.75221 ABIN6146897 WB Rabbit Log in to see Polyclonal 0
12.024078 ABIN3184045 ELISA WB Rabbit IgG C-Term Log in to see Polyclonal 0
12.024078 ABIN3182370 ELISA WB Rabbit IgG pSer387 Log in to see Polyclonal 0
12.024078 ABIN5621213 ELISA WB Rabbit IgG Log in to see Polyclonal 0
12.024078 ABIN2150542 IHC ELISA WB Rabbit IgG Log in to see Polyclonal 0
12.024078 ABIN2150543 ELISA WB Rabbit IgG pSer385 Log in to see Polyclonal 0
12.024078 ABIN2150544 ELISA WB Rabbit IgG Ser387 Log in to see Polyclonal 0
12.024078 ABIN6135081 WB Rabbit Log in to see Polyclonal 0
10.728131 ABIN6259497 ELISA ICC IF WB Rabbit IgG Log in to see Polyclonal 0
9.228131 ABIN1881747 WB Rabbit AA 672-701, C-Term Log in to see Polyclonal 3
7 ABIN5538462 FACS WB Rabbit Ig Fraction AA 37-65, N-Term Log in to see Polyclonal 0
4.7281313 ABIN2774775 WB Rabbit C-Term Log in to see Polyclonal 0
4.7281313 ABIN2775650 WB Rabbit Middle Region Log in to see Polyclonal 0
4.7281313 ABIN2801443 IP WB Rabbit pSer387 Log in to see Polyclonal 0
4.7281313 ABIN1532168 ELISA WB Rabbit IgG AA 353-402, pSer387 Log in to see Polyclonal 1
4.7281313 ABIN1534866 ELISA WB Rabbit IgG AA 661-710 Log in to see Polyclonal 1
4.7281313 ABIN655275 FACS WB Rabbit Ig Fraction AA 37-65, N-Term Log in to see Polyclonal 0
4.7281313 ABIN5962361 ELISA WB Rabbit IgG Ser387 Log in to see Polyclonal 0

General

Antigen Ring Finger and WD Repeat Domain 2, E3 Ubiquitin Protein Ligase (RFWD2) Antibodies
Epitope AA 632-732
(18), (16), (10), (8), (7), (4), (3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine)
(80), (36), (7), (4), (4), (3), (3), (3), (2), (2), (1), (1), (1), (1)
Host Mouse
(77), (3), (2)
Clonality (Clone)
Monoclonal   ( )
Conjugate This RFWD2 antibody is un-conjugated
(3), (3), (3), (3), (3), (3)
Application Immunofluorescence (IF), Western Blotting (WB)
(80), (53), (8), (5), (4), (3), (1)
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Product Details anti-RFWD2 Antibody

Target Details RFWD2 Application Details Handling Images
Cross-Reactivity (Details) Human: ~~ Positive
Purification This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Immunogen

Antigen Type: Recombinant Protein

Target/Specificity: RFWD2 (NP_071902, 632 a.a. ~ 731 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Clone 1E4
Isotype IgG2b kappa
Plasmids, Primers & others

Target Details RFWD2

Product Details anti-RFWD2 Antibody Application Details Handling Images back to top
Antigen
Alternative Name RFWD2 (RFWD2 Antibody Abstract)
Background

Other Names: E3 ubiquitin-protein ligase RFWD2, 632-, Constitutive photomorphogenesis protein 1 homolog, hCOP1, RING finger and WD repeat domain protein 2, RING finger protein 200, RFWD2, COP1, RNF200

Gene Description: ring finger and WD repeat domain 2

Molecular Weight 80474 DA
Gene ID 64326
NCBI Accession NP_071902, NP_001001740
UniProt Q8NHY2
Pathways Photoperiodism

Application Details

Product Details anti-RFWD2 Antibody Target Details RFWD2 Handling Images back to top
Application Notes Western blot = 1:500-1000
Restrictions For Research Use only

Handling

Product Details anti-RFWD2 Antibody Target Details RFWD2 Application Details Images back to top
Buffer Clear, colorless solution in phosphate buffered saline,  pH 7.2 .
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Expiry Date 6 months

Images

Product Details anti-RFWD2 Antibody Target Details RFWD2 Application Details Handling back to top
Supplier Images
Western Blotting (WB) image for anti-Ring Finger and WD Repeat Domain 2, E3 Ubiquitin Protein Ligase (RFWD2) (AA 632-732) antibody (ABIN394529) Immunofluorescence of monoclonal antibody to RFWD2 on NIH/3T3 cell (antibody concentr...
Immunohistochemistry (IHC) image for anti-Ring Finger and WD Repeat Domain 2, E3 Ubiquitin Protein Ligase (RFWD2) (AA 632-732) antibody (ABIN394529) anti-Ring Finger and WD Repeat Domain 2, E3 Ubiquitin Protein Ligase (RFWD2) (AA 632-732) antibody (Image 2)
Western Blotting (WB) image for anti-Ring Finger and WD Repeat Domain 2, E3 Ubiquitin Protein Ligase (RFWD2) (AA 632-732) antibody (ABIN394529) Antibody Reactive Against Recombinant Protein.Western Blot detection against Immunoge...
Western Blotting (WB) image for anti-Ring Finger and WD Repeat Domain 2, E3 Ubiquitin Protein Ligase (RFWD2) (AA 632-732) antibody (ABIN394529) RFWD2 monoclonal antibody (M01), clone 1E4. Western Blot analysis of RFWD2 expression...
Western Blotting (WB) image for anti-Ring Finger and WD Repeat Domain 2, E3 Ubiquitin Protein Ligase (RFWD2) (AA 632-732) antibody (ABIN394529) RFWD2 monoclonal antibody (M01), clone 1E4 Western Blot analysis of RFWD2 expression ...
Immunofluorescence (IF) image for anti-Ring Finger and WD Repeat Domain 2, E3 Ubiquitin Protein Ligase (RFWD2) (AA 632-732) antibody (ABIN394529) Immunofluorescence of monoclonal antibody to RFWD2 on NIH/3T3 cell. [antibody concent...
ELISA image for anti-Ring Finger and WD Repeat Domain 2, E3 Ubiquitin Protein Ligase (RFWD2) (AA 632-732) antibody (ABIN394529) Detection limit for recombinant GST tagged RFWD2 is approximately 0.1ng/ml as a captu...