anti-Mouse (Murine) BIM antibody for Immunohistochemistry

Recommended BIM Antibody

Antigen
BCL2-Like 11 (Apoptosis Facilitator) (BCL2L11) Antibodies
  • bim
  • BAM
  • BIM
  • BOD
  • 1500006F24Rik
  • Bim
  • Bod
  • BimL
  • BIMEL
  • BCL2-like 11
  • BCL2 like 11
  • BCL2-like 11 (apoptosis facilitator)
  • bcl2l11
  • BCL2L11
  • Bcl2l11
Reactivity
Human, Mouse (Murine), Rat (Rattus)
443
316
296
11
6
6
4
3
3
1
1
Host
Rabbit
403
18
17
9
6
Clonality
Polyclonal
Conjugate
This BIM antibody is conjugated to PE-Atto 594
25
15
12
12
11
11
10
10
10
10
10
10
6
6
6
6
6
6
6
6
6
6
3
2
2
2
2
2
2
2
2
2
2
2
2
1
Application
Immunohistochemistry (IHC), Western Blotting (WB)
330
182
114
63
61
38
36
28
25
17
7
5
3
3
2
2
2
2
Options

Available images

Catalog No. ABIN2485940
$ 477.10
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Clonality References Details
22.643246 ABIN2485927 IHC WB Atto 390 Rabbit Polyclonal 2
22.643246 ABIN2485928 IHC WB Atto 488 Rabbit Polyclonal 2
22.643246 ABIN2485929 IHC WB Atto 565 Rabbit Polyclonal 2
22.643246 ABIN2485930 IHC WB Atto 594 Rabbit Polyclonal 2
22.643246 ABIN2485932 IHC WB Atto 655 Rabbit Polyclonal 2
22.643246 ABIN2485933 IHC WB Atto 680 Rabbit Polyclonal 2
22.643246 ABIN2485934 IHC WB Atto 700 Rabbit Polyclonal 2
22.643246 ABIN2485936 IHC WB APC Rabbit Polyclonal 2
22.643246 ABIN2485931 IHC WB Atto 633 Rabbit Polyclonal 2
22.643246 ABIN2485938 IHC WB FITC Rabbit Polyclonal 2
22.643246 ABIN2485941 IHC WB PerCP Rabbit Polyclonal 2
22.643246 ABIN2485942 IHC WB PE Rabbit Polyclonal 2
22.643246 ABIN2485943 IHC WB Streptavidin Rabbit Polyclonal 2
22.643246 ABIN2485935 IHC WB Alkaline Phosphatase (AP) Rabbit Polyclonal 2
22.643246 ABIN2485937 IHC WB Biotin Rabbit Polyclonal 2
22.643246 ABIN2485939 IHC WB HRP Rabbit Polyclonal 2
19.0936 ABIN6271662 ELISA IHC WB Rabbit IgG pSer104, pSer44 Polyclonal 0
19.0936 ABIN6260264 ELISA IHC WB Rabbit IgG Polyclonal 0
18.049648 ABIN965668 IHC Rabbit pSer69, pSer65 2
18.049648 ABIN965669 IHC Rabbit Polyclonal 2

General

Antigen BCL2-Like 11 (Apoptosis Facilitator) (BCL2L11) Antibodies
Reactivity Human, Mouse (Murine), Rat (Rattus)
(443), (316), (296), (11), (6), (6), (4), (3), (3), (1), (1)
Host Rabbit
(403), (18), (17), (9), (6)
Clonality
Polyclonal
Conjugate This BIM antibody is conjugated to PE-Atto 594
(25), (15), (12), (12), (11), (11), (10), (10), (10), (10), (10), (10), (6), (6), (6), (6), (6), (6), (6), (6), (6), (6), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1)
Application Immunohistochemistry (IHC), Western Blotting (WB)
(330), (182), (114), (63), (61), (38), (36), (28), (25), (17), (7), (5), (3), (3), (2), (2), (2), (2)
Pubmed 2 references available
Supplier

Product Details anti-BIM Antibody

Target Details BIM Application Details Handling References for anti-BIM antibody (ABIN2485940) Images
Specificity Detects ~23 kDa.
Purification Protein A Purified
Immunogen Internal central amino acids of human Bim
Plasmids, Primers & others

Target Details BIM

Product Details anti-BIM Antibody Application Details Handling References for anti-BIM antibody (ABIN2485940) Images back to top
Antigen
Alternative Name BIM/BOD (BCL2L11 Antibody Abstract)
Background Members in the Bcl-2 family are critical regulators of apoptosis by either inhibiting or promoting cell death. Bim/BOD is a group of three splice variants, BimEL, BimL and BimS, with apparent molecular masses of ~23, 16, and 13 kDa, respectively. Bcl-2 homology 3 (BH3) domain is a potent death domain. BH3 domain containing pro-apoptotic proteins, including Bad, Bax, Bid, Bik, and Hrk, form a growing subclass of the Bcl-2 family. A novel BH3 domain containing protein was recently identified and designated Bim or BOD in human, mouse and rat (1,2). Bim/BOD interacts with diverse members in the pro-survival Bcl-2 sub-family including Bcl-2, Bcl-xL and Bcl-w. Bim/BOD induces apoptosis. The messenger RNA of Bim is ubiquitously expressed in multiple tissues and cell lines (1 ,2).
Gene ID 10018
NCBI Accession NP_619527
UniProt O43521
Pathways PI3K-Akt Signaling, Neurotrophin Signaling Pathway, Tube Formation, Positive Regulation of Endopeptidase Activity

Application Details

Product Details anti-BIM Antibody Target Details BIM Handling References for anti-BIM antibody (ABIN2485940) Images back to top
Application Notes
  • WB (1:1000)
  • IHC (1:100)
  • optimal dilutions for assays should be determined by the user.
Comment

1 μg/ml of SPC-113 was sufficient for detection of Bim in 20 μg of K562 and A549 cell lysates by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.

Restrictions For Research Use only

Handling

Product Details anti-BIM Antibody Target Details BIM Application Details References for anti-BIM antibody (ABIN2485940) Images back to top
Format Liquid
Concentration 1 mg/mL
Buffer PBS, 50 % glycerol, 0.09 % sodium azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
Storage -20 °C

References for anti-BIM antibody (ABIN2485940)

Product Details anti-BIM Antibody Target Details BIM Application Details Handling Images back to top
Background publications

OConnor, Strasser, OReilly, Hausmann, Adams, Cory, Huang: "Bim: a novel member of the Bcl-2 family that promotes apoptosis." in: The EMBO journal, Vol. 17, Issue 2, pp. 384-95, 1998

Hsu, Lin, Hsueh: "BOD (Bcl-2-related ovarian death gene) is an ovarian BH3 domain-containing proapoptotic Bcl-2 protein capable of dimerization with diverse antiapoptotic Bcl-2 members." in: Molecular endocrinology (Baltimore, Md.), Vol. 12, Issue 9, pp. 1432-40, 1998

Images

Product Details anti-BIM Antibody Target Details BIM Application Details Handling References for anti-BIM antibody (ABIN2485940) back to top
Supplier Images
Immunohistochemistry (IHC) image for anti-BCL2-Like 11 (Apoptosis Facilitator) (BCL2L11) antibody (PE-Atto 594) (ABIN2485940) Immunohistochemistry analysis using Rabbit Anti-BIM Polyclonal Antibody . Tissue: ski...
Western Blotting (WB) image for anti-BCL2-Like 11 (Apoptosis Facilitator) (BCL2L11) antibody (PE-Atto 594) (ABIN2485940) Western blot analysis of Human K562 (Left) and A549 (Right) whole cell lysates showin...