anti-Monkey Cyclin D1 antibody for Western Blotting

Recommended Cyclin D1 Antibody (supplied by: Log in to see )

Antigen
Cyclin D1 (CCND1) Antibodies
  • BCL1
  • D11S287E
  • PRAD1
  • U21B31
  • AI327039
  • CycD1
  • Cyl-1
  • bcl-1
  • cD1
  • cb161
  • cycd1
  • etID37810.7
  • fb52e01
  • fc45c08
  • fc83a12
  • id:ibd1198
  • wu:fb52e01
  • wu:fc45c08
  • wu:fc83a12
  • ccnd1b
  • prad1
  • ccnd1
  • ccnd1a
  • bcl1
  • d11s287e
  • u21b31
  • CCND1
  • cyclin D1
  • cyclin D1 S homeolog
  • cyclin D1 L homeolog
  • cyclin D2
  • CCND1
  • Ccnd1
  • ccnd1
  • ccnd1.S
  • ccnd1.L
  • CCND2
Reactivity
Human, Monkey, Mouse (Murine), Rat (Rattus)
518
281
250
41
40
19
18
17
16
4
4
4
4
4
3
3
2
2
2
2
2
1
1
1
1
1
1
Host
Mouse
361
239
19
6
Clonality
Monoclonal
Conjugate
This Cyclin D1 antibody is un-conjugated
31
27
19
13
11
8
7
4
4
4
4
4
4
4
4
4
4
4
4
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
Application
Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
546
201
159
142
141
129
103
78
47
47
22
8
7
6
3
3
1
1
Supplier
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Catalog No. ABIN3026634
$ 361.90
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
3.1493068 ABIN3024574 FACS IF IHC (p) WB Mouse IgG2a, kappa Log in to see SPM587 0
1 ABIN470384 WB Rabbit C-Term Log in to see Polyclonal 0
1 ABIN490790 ELISA ICC IHC IP WB Rabbit C-Term Log in to see Polyclonal 0
1 ABIN6161217 FACS IF IHC (f) WB CF®660R Mouse IgG2a, kappa Log in to see DCS-6 0
1 ABIN6161219 FACS IF IHC (f) WB CF®568 Mouse IgG2a, kappa Log in to see DCS-6 0
1 ABIN6161227 FACS IF IHC (f) WB CF®488A Mouse IgG2a, kappa Log in to see DCS-6 0
1 ABIN6161223 FACS IF IHC (f) WB CF®680 Mouse IgG2a, kappa Log in to see DCS-6 0
1 ABIN6161209 FACS IF IHC (f) WB CF®640R Mouse IgG2a, kappa Log in to see DCS-6 0
1 ABIN6161207 FACS IF IHC (f) WB CF405M Mouse IgG2a, kappa Log in to see DCS-6 0
1 ABIN6161211 FACS IF IHC (f) WB CF®543 Mouse IgG2a, kappa Log in to see DCS-6 0
1 ABIN6161225 FACS IF IHC (f) WB CF®680R Mouse IgG2a, kappa Log in to see DCS-6 0
1 ABIN6161229 FACS IF IHC (f) WB CF®594 Mouse IgG2a, kappa Log in to see DCS-6 0
1 ABIN6161221 FACS IF IHC (f) WB CF®770 Mouse IgG2a, kappa Log in to see DCS-6 0
1 ABIN6161205 FACS IF IHC (f) WB CF®405S Mouse IgG2a, kappa Log in to see DCS-6 0
1 ABIN6161213 FACS IF IHC (f) WB CF®647 Mouse IgG2a, kappa Log in to see DCS-6 0
1 ABIN6161215 FACS IF IHC (f) WB CF®555 Mouse IgG2a, kappa Log in to see DCS-6 0
1 ABIN6161240 FACS IF IHC (f) WB Mouse IgG2a, kappa Log in to see DCS-6 0
1 ABIN6161234 FACS IF IHC (f) WB Biotin Mouse IgG2a, kappa Log in to see DCS-6 0
1 ABIN6161232 FACS IF IHC (f) WB Alkaline Phosphatase (AP) Mouse IgG2a, kappa Log in to see DCS-6 0
1 ABIN6161231 FACS IF IHC (f) WB APC Mouse IgG2a, kappa Log in to see DCS-6 0

General

Antigen Cyclin D1 (CCND1) Antibodies
Reactivity Human, Monkey, Mouse (Murine), Rat (Rattus)
(518), (281), (250), (41), (40), (19), (18), (17), (16), (4), (4), (4), (4), (4), (3), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Host Mouse
(361), (239), (19), (6)
Clonality (Clone)
Monoclonal   ( )
Conjugate This Cyclin D1 antibody is un-conjugated
(31), (27), (19), (13), (11), (8), (7), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1)
Application Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(546), (201), (159), (142), (141), (129), (103), (78), (47), (47), (22), (8), (7), (6), (3), (3), (1), (1)
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Product Details anti-Cyclin D1 Antibody

Target Details Cyclin D1 Application Details Handling Images
Characteristics This antibody recognizes a protein of 36 kDa, identified as Cyclin D1. It is a putative proto-oncogene overexpressed in a wide variety of human neoplasms and a key cell cycle regulator. This antibody neutralizes the activity of Cyclin D1 in vivo. About 60 % of mantle cell lymphomas (MCL) contain a t(11, 14)(q13, q32) translocation resulting in over-expression. Cyclin D1 antibody is useful in identifying mantle cell lymphomas, which stain positive, from CLL/SLL and follicular lymphomas, which stain negative. Occasionally, hairy cell leukemia and plasma cell myeloma weakly express Cyclin D1.
Purification Protein G affinity chromatography
Immunogen Human recombinant full length protein was used as the immunogen for this Cyclin D1 antibody.
Clone DCS-6
Isotype IgG2a, kappa
Plasmids, Primers & others

Target Details Cyclin D1

Product Details anti-Cyclin D1 Antibody Application Details Handling Images back to top
Antigen
Alternative Name Cyclin D1 (CCND1 Antibody Abstract)
Background This antibody recognizes a protein of 36  kDa, identified as Cyclin D1. It is a putative proto-oncogene overexpressed in a wide variety of human neoplasms and a key cell cycle regulator. This antibody neutralizes the activity of Cyclin D1 in vivo. About 60 % of mantle cell lymphomas (MCL) contain a t(11, 14)(q13, q32) translocation resulting in over-expression. Cyclin D1 antibody is useful in identifying mantle cell lymphomas, which stain positive, from CLL/SLL and follicular lymphomas, which stain negative. Occasionally, hairy cell leukemia and plasma cell myeloma weakly express Cyclin D1.
Gene ID 595
Pathways PI3K-Akt Signaling, Cell Division Cycle, Mitotic G1-G1/S Phases, ER-Nucleus Signaling

Application Details

Product Details anti-Cyclin D1 Antibody Target Details Cyclin D1 Handling Images back to top
Application Notes The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the Cyclin D1 antibody to be titered up or down for optimal performance.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. FACS: 0.5-1 μg/million cells,IF: 1-2 μg/mL,WB: 0.5-1 μg/mL,IHC (FFPE): 0.5-1 μg/mL for 30 minutes at RT (1),Prediluted format : incubate for 30 min at RT (2)

Restrictions For Research Use only

Handling

Product Details anti-Cyclin D1 Antibody Target Details Cyclin D1 Application Details Images back to top
Concentration 0.2 mg/mL
Buffer 0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C,-20 °C
Storage Comment Store the Cyclin D1 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Images

Product Details anti-Cyclin D1 Antibody Target Details Cyclin D1 Application Details Handling back to top
Supplier Images
Flow Cytometry (FACS) image for anti-Cyclin D1 (CCND1) antibody (ABIN3026634) FACS testing of Jurkat cells: Black=cells alone; Green=isotype control; Red=Cyclin D1...
Flow Cytometry (FACS) image for anti-Cyclin D1 (CCND1) antibody (ABIN3026634) FACS testing of MCF-7 cells: Black=cells alone; Green=isotype control; Red=Cyclin D1 ...
Immunohistochemistry (IHC) image for anti-Cyclin D1 (CCND1) antibody (ABIN3026634) IHC testing of human mantle cell lymphoma stained with Cyclin D1 antibody. Nuclear s...
Western Blotting (WB) image for anti-Cyclin D1 (CCND1) antibody (ABIN3026634) Western blot testing of human and mouse samples with DCS-6 mAb.