Mouse (Murine) PPP2R4 ELISA Kit

Recommended PPP2R4 Product (supplied by: Log in to see )

Antigen
Protein Phosphatase 2A Activator, Regulatory Subunit 4 (PPP2R4) ELISA Kits
  • 6330556D22Rik
  • PP2A
  • PR65
  • PR53
  • PTPA
  • PTPRA
  • 2610042B21Rik
  • C77440
  • N28142
  • protein phosphatase 2, regulatory subunit A, alpha
  • protein phosphatase 2 phosphatase activator
  • protein phosphatase 2 protein activator
  • Ppp2r1a
  • PTPA
  • Ptpa
Epitope
Regulatory Subunit 4
Reactivity
Mouse (Murine)
Alternatives
Kits with alternative reactivity to:
6
2
1
1
Method Type
Sandwich ELISA
Detection Range
0.156 ng/mL - 10 ng/mL
Minimum Detection Limit
0.156 ng/mL
Application
ELISA
Supplier
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Catalog No. ABIN5674923
$ 929.89
Plus shipping costs $45.00
Relevance Score ABIN Method Type Sample Type Detection Range Detection Minimum Supplier References Details
1 ABIN1157493 Log in to see

General

Antigen Protein Phosphatase 2A Activator, Regulatory Subunit 4 (PPP2R4) ELISA Kits
Epitope Regulatory Subunit 4
Reactivity Mouse (Murine)
Kits with alternative reactivity to:
(6), (2), (1), (1)
Method Type Sandwich ELISA
Detection Range 0.156 ng/mL - 10 ng/mL
Minimum Detection Limit 0.156 ng/mL
Application ELISA
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Product Details PPP2R4 ELISA Kit

Target Details Application Details Handling Images
Sample Type Tissue Homogenate
Detection Method Colorimetric
Analytical Method Quantitative
Sensitivity 0062 ng/mL
Components Pre-coated, ready to use 96-well strip plate
Standard (freeze dried)
Standard Diluent
Detection Reagent A
Detection Reagent B
Assay Diluent A
Assay Diluent B
TMB Substhumane
Stop Solution
Wash Buffer(30 x concenthumane)
Plate sealer for 96 wells
Instruction manual
Material not included 1. Microplate reader with 450 ± 10nm filter.
2. Precision single or multi-channel pipettes and disposable tips.
3. Eppendorf Tubes for diluting samples.
4. Deionized or distilled water.
5. Absorbent paper for blotting the microtiter plate.
6. Container for Wash Solution.

Target Details

Product Details PPP2R4 ELISA Kit Application Details Handling Images back to top
Antigen
Alternative Name Protein Phosphatase 2A Activator, (PPP2R4) (PPP2R4 ELISA Kit Abstract)
Background Alternative name: PP2A, PR53, PTPA, Phosphotyrosyl Phosphatase Activator, PP2A Phosphatase Activator, PP2A, subunit B', PR53 isoform, Serine/threonine-protein phosphatase 2A activator
Gene ID 110854
UniProt P58389
Pathways PI3K-Akt Signaling, M Phase, Hepatitis C, Toll-Like Receptors Cascades

Application Details

Product Details PPP2R4 ELISA Kit Target Details Handling Images back to top
Application Notes Optimal working dilution should be determined by the investigator.
Assay Time 1 - 4.5 h
Plate Pre-coated
Protocol 1. Prepare all reagents, samples and standards
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37℃
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37℃
4. Aspirate and wash 3 times
5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37℃
6. Aspirate and wash 5 times
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37℃
8. Add 50µL Stop Solution. Read at 450nm immediately.
Restrictions For Research Use only

Handling

Product Details PPP2R4 ELISA Kit Target Details Application Details Images back to top
Storage 4 °C
Expiry Date 12 months