anti-Human BLK antibody for Western Blotting

Recommended BLK Antibody

Antigen
B Lymphoid Tyrosine Kinase (BLK) Antibodies
  • bltk
  • si:dkey-33i22.2
  • zgc:136231
  • MODY11
  • BLK proto-oncogene, Src family tyrosine kinase
  • B lymphoid kinase
  • blk
  • BLK
  • Blk
Reactivity
Human
170
79
51
3
2
1
1
1
1
1
1
Host
Mouse
114
46
16
Clonality
Monoclonal
Conjugate
This BLK antibody is un-conjugated
6
4
4
3
3
3
3
3
3
3
3
3
1
1
1
1
1
Application
Immunocytochemistry (ICC), Flow Cytometry (FACS), ELISA, Western Blotting (WB)
123
103
74
22
13
8
6
5
4
3
1
1
Options

Available images

Catalog No. ABIN968983
$ 393.80
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Clonality References Details
24.93992 ABIN968981 IHC ELISA WB Mouse IgG1 9D10B7 1
24.93992 ABIN968982 IHC ELISA WB Mouse IgG1 9D10B7H6 1
21.390388 ABIN560064 ELISA WB Mouse IgG1 kappa AA 1-90 7A12 2
20.43992 ABIN931403 IHC ELISA WB Mouse IgG1 9D10B7H6?9D10A8F8 0
18.049534 ABIN965673 IHC ELISA WB IgG2a 1
18.049534 ABIN965674 IHC ELISA WB IgG1 1
17.43992 ABIN2854388 WB Rabbit IgG Internal Region Polyclonal 0
17.43992 ABIN630217 WB Rabbit Middle Region Polyclonal 0
13.890388 ABIN6271432 ELISA IHC WB Rabbit IgG pTyr389 Polyclonal 0
13.890388 ABIN1842966 ELISA FACS IF/ICC WB Mouse IgG1 0
13.890388 ABIN560063 ELISA WB Mouse IgG1 kappa AA 1-505 3E5-3A8 0
13.549533 ABIN5539479 ELISA WB Goat N-Term Polyclonal 0
13.549533 ABIN4901081 ELISA IHC (p) WB Mouse IgG1 9D10B7 0
13.549533 ABIN4901082 ELISA IHC (p) WB Mouse IgG1 9D10B7H6-9D10A8F8 0
13.549533 ABIN4901083 ELISA FACS IF WB Mouse IgG1 1E6 0
13.549533 ABIN931401 IHC ELISA WB Mouse IgG1 9D10B7 0
13.549533 ABIN5621805 ELISA FACS IF WB Mouse IgG1 M72765 0
13.549533 ABIN2134765 IHC ELISA WB Rabbit IgG Polyclonal 0
13.549533 ABIN6137572 WB Rabbit Polyclonal 0
13.549533 ABIN965675 IHC ELISA WB IgG1 1

General

Antigen B Lymphoid Tyrosine Kinase (BLK) Antibodies
Reactivity Human
(170), (79), (51), (3), (2), (1), (1), (1), (1), (1), (1)
Host Mouse
(114), (46), (16)
Clonality (Clone)
Monoclonal   ( )
Conjugate This BLK antibody is un-conjugated
(6), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (1), (1), (1), (1), (1)
Application Immunocytochemistry (ICC), Flow Cytometry (FACS), ELISA, Western Blotting (WB)
(123), (103), (74), (22), (13), (8), (6), (5), (4), (3), (1), (1)
Pubmed 3 references available
Supplier

Product Details anti-BLK Antibody

Target Details BLK Application Details Handling References for anti-BLK antibody (ABIN968983) Images
Purification Ascites
Immunogen Purified recombinant fragment of human BLK expressed in E. Coli.
Clone 1-00E-06
Isotype IgG1
Plasmids, Primers & others

Target Details BLK

Product Details anti-BLK Antibody Application Details Handling References for anti-BLK antibody (ABIN968983) Images back to top
Antigen
Alternative Name BLK (BLK Antibody Abstract)
Background Blk is a Src family protein tyrosine kinase expressed in all stages of B cell development . Activation of B cells by various ligands is accompanied by activation of Blk . It has been suggested that Blk is involved in the control of B cell differentiation and proliferation . Blk transcripts have also been detected in human thymocytes, but not in mature T cells, implicating that Blk may play an important role in thymopoiesis . Blk function may be redundant, however, as mice that do not express Blk are not impaired with respect to B cell development and immune response .
Molecular Weight 58 kDa
Gene ID 640
HGNC 640
Pathways Positive Regulation of Peptide Hormone Secretion, CXCR4-mediated Signaling Events, Thromboxane A2 Receptor Signaling

Application Details

Product Details anti-BLK Antibody Target Details BLK Handling References for anti-BLK antibody (ABIN968983) Images back to top
Application Notes ELISA: 1:10000, WB: 1:500 - 1:2000, ICC: 1:200 - 1:1000, FCM: 1:200 - 1:400
Restrictions For Research Use only

Handling

Product Details anti-BLK Antibody Target Details BLK Application Details References for anti-BLK antibody (ABIN968983) Images back to top
Format Liquid
Buffer Ascitic fluid containing 0.03 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C,-20 °C
Storage Comment 4°C, -20°C for long term storage

References for anti-BLK antibody (ABIN968983)

Product Details anti-BLK Antibody Target Details BLK Application Details Handling Images back to top
Product cited in:

Yang, Ng, Zhao, Hirankarn, Lau, Mok, Chan, Wong, Lee, Mok, Wong, Avihingsanon, Lee, Ho, Lee, Wong, Lau: "Population differences in SLE susceptibility genes: STAT4 and BLK, but not PXK, are associated with systemic lupus erythematosus in Hong Kong Chinese." in: Genes and immunity, Vol. 10, Issue 3, pp. 219-26, 2009

Borowiec, Liew, Thompson, Boonyasrisawat, Hu, Mlynarski, El Khattabi, Kim, Marselli, Rich, Krolewski, Bonner-Weir, Sharma, Sale, Mychaleckyj, Kulkarni, Doria: "Mutations at the BLK locus linked to maturity onset diabetes of the young and beta-cell dysfunction." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 106, Issue 34, pp. 14460-5, 2009

Hom, Graham, Modrek, Taylor, Ortmann, Garnier, Lee, Chung, Ferreira, Pant, Ballinger, Kosoy, Demirci, Kamboh, Kao, Tian, Gunnarsson, Bengtsson, Rantapää-Dahlqvist, Petri, Manzi, Seldin, Rönnblom et al.: "Association of systemic lupus erythematosus with C8orf13-BLK and ITGAM-ITGAX. ..." in: The New England journal of medicine, Vol. 358, Issue 9, pp. 900-9, 2008

Images

Product Details anti-BLK Antibody Target Details BLK Application Details Handling References for anti-BLK antibody (ABIN968983) back to top
Supplier Images
Flow Cytometry (FACS) image for anti-B Lymphoid Tyrosine Kinase (BLK) antibody (ABIN968983) Figure 1: Flow cytometric analysis of HL-60 cells using BLK mouse mAb (green) and neg...
Immunocytochemistry (ICC) image for anti-B Lymphoid Tyrosine Kinase (BLK) antibody (ABIN968983) Figure 2: Immunofluorescence analysis of Hela cells using BLK mouse mAb (green) . Blu...
Western Blotting (WB) image for anti-B Lymphoid Tyrosine Kinase (BLK) antibody (ABIN968983) Figure 3: Western blot analysis using BLK mAb against HEK293 (1) and BLK (AA: 2-200) ...