IL12A Protein (AA 23-219) (His tag)

Catalog No. ABIN1000979

Product Details

Target: IL12A (Interleukin 12 alpha (IL12A))

Protein Type: Recombinant

Protein Characteristics: AA 23-219

Origin: Human

Source: Escherichia coli (E. coli)

Purification tag / Conjugate: This IL12A protein is labelled with His tag.

Application: Western Blotting (WB), Control (Ct)

Purpose: Interleukin-12p35 (IL-12p35) (Western Blot Control)

Sequence: RNLPVATPDP GMFPCLHHSQ NLLRAVSNML QKARQTLEFY RNLPVATPDP GMFPCLHHSQ NLLRAVSNML QKARQTLEFY RNLPVATPDP GMFPCLHHSQ NLLRAVSNML QKARQTLEFY RNLPVATPDP GMFPCLHHSQ NLLRAVSNML QKARQTLEFY LEEPDFYKTK IKLCILLHAF RIRAVTIDRV MSYLNAS

Characteristics: recombinant Interleukin-12p35 comprises a 197 amino acid fragment (23-219) corresponding to the mature Interleukin-12p35 chain protein and is expressed in E. coli with an amino-terminal hexahistidine tag.

Purity: > 95 %

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Supplied in 1x Laemmli Buffer (25 mM Tris-HCl pH 6.8, 50 mM DTT, 1 % (w/v) SDS, 0.1 % (w/v) Bromophenol Blue, 2.5 % Glycerol).

Preservative: Dithiothreitol (DTT)

Precaution of Use: This product contains Dithiothreitol (DTT): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C

Target Details

Target: IL12A (Interleukin 12 alpha (IL12A))

Alternative Name: Interleukin-12 p35 (IL12A Products)

Synonyms: CLMF Protein, IL-12A Protein, NFSK Protein, NKSF1 Protein, P35 Protein, CLMF2 Protein, IL-12B Protein, NKSF Protein, NKSF2 Protein, p40 Protein, Il-12b Protein, Il12p40 Protein, Il-12p40 Protein, Il12 Protein, IL-12p40 Protein, IL-12p35 Protein, Il-12a Protein, Ll12a Protein, p35 Protein, IL12p35 Protein, Il-12 p35 Protein, IL12p40 Protein, IL12 Protein, IL-12 p35 Protein, interleukin 12A Protein, interleukin 12B Protein, interleukin 12b Protein, interleukin 12a Protein, Interleukin 12 level Protein, IL12A Protein, IL12B Protein, Il12b Protein, Il12a Protein, IL12 Protein, il12a Protein

Background: Active IL-12 is a p70 disulphide-linked dimer composed of p35 and p40 subunits. The protein is a pleiotropic cytokine produced primarily by antigen presenting cells and has multiple effects on T lymphocytes and natural killer cells in terms of stimulating cytotoxicity, proliferation, production of other cytokines and Th1 subset differentiation.,IL-12A, Cytotoxic Lymphocyte Maturation Factor, CLMF, Natural Killer Cell Stimulation Factor 1, NKSF1, NFSK, subunit p35.

Molecular Weight: 27.04 kDa

UniProt: P29459

Pathways: JAK-STAT Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Activated T Cell Proliferation