BAI3 Protein (AA 25-880) (His tag)

Catalog No. ABIN1096080

Product Details

Target: BAI3 (Brain-Specific Angiogenesis Inhibitor 3 (BAI3))

Protein Type: Recombinant

Protein Characteristics: AA 25-880

Origin: Human

Source: Human Cells

Purification tag / Conjugate: This BAI3 protein is labelled with His tag.

Purpose: Recombinant Human Brain-Specific Angiogenesis Inhibitor 3/BAI3 (C-6His)

Sequence: AQDFWCSTLV KGVIYGSYSV SEMFPKNFTN CTWTLENPDP TKYSIYLKFS KKDLSCSNFS LLAYQFDHFS HEKIKDLLRK NHSIMQLCNS KNAFVFLQYD KNFIQIRRVF PTNFPGLQKK GEEDQKSFFE FLVLNKVSPS QFGCHVLCTW LESCLKSENG RTESCGIMYT KCTCPQHLGE WGIDDQSLIL LNNVVLPLNE QTEGCLTQEL QTTQVCNLTR EAKRPPKEEF GMMGDHTIKS QRPRSVHEKR VPQEQADAAK FMAQTGESGV EEWSQWSTCS VTCGQGSQVR TRTCVSPYGT HCSGPLRESR VCNNTALCPV HGVWEEWSPW SLCSFTCGRG QRTRTRSCTP PQYGGRPCEG PETHHKPCNI ALCPVDGQWQ EWSSWSQCSV TCSNGTQQRS RQCTAAAHGG SECRGPWAES RECYNPECTA NGQWNQWGHW SGCSKSCDGG WERRIRTCQG AVITGQQCEG TGEEVRRCSE QRCPAPYEIC PEDYLMSMVW KRTPAGDLAF NQCPLNATGT TSRRCSLSLH GVAFWEQPSF ARCISNEYRH LQHSIKEHLA KGQRMLAGDG MSQVTKTLLD LTQRKNFYAG DLLMSVEILR NVTDTFKRAS YIPASDGVQN FFQIVSNLLD EENKEKWEDA QQIYPGSIEL MQVIEDFIHI VGMGMMDFQN SYLMTGNVVA SIQKLPAASV LTDINFPMKG RKGMVDWARN SEDRVVIPKS IFTPVSSKEL DESSVFVLGA VLYKNLDLIL PTLRNYTVIN SKIIVVTIRP EPKTTDSFLE IELAHLANGT LNPYCVLWDD SKTNESLGTW STQGCKTVLT DASHTKCLCD RLSTFAILAQ QPREIIMESS GTPSVTVDHH HHHH

Characteristics: Recombinant Human Brain-Specific Angiogenesis Inhibitor 3/BAI3 produced by transfected human cells is a secreted protein with sequence (Ala25-Thr880) of Human BAI3 fused with a polyhistidine tag at the C-terminus.

Purity: > 95 % as determined by reducing SDS-PAGE.

Sterility: 0.2 μm filtered

Endotoxin Level: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Buffer: Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, 5 % Thehalose, pH 7.2.

Handling Advice: Always centrifuge tubes before opening. Do not mix by vortex or pipetting.

Storage: 4 °C/-20 °C/-80 °C

Storage Comment: Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7°C for 2-7 days.
Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Expiry Date: 3 months

Target Details

Target: BAI3 (Brain-Specific Angiogenesis Inhibitor 3 (BAI3))

Alternative Name: bai3 (BAI3 Products)

Synonyms: zgc:163001 Protein, BAI3 Protein, A830096D10Rik Protein, adhesion G protein-coupled receptor B3 Protein, brain-specific angiogenesis inhibitor 3 Protein, ADGRB3 Protein, Adgrb3 Protein, adgrb3 Protein, BAI3 Protein

Sub Type: Fusionprotein

Background: Human Brain-Specific Angiogenesis Inhibitor 3 (BAI3) is a 177 kDa seven-span transmembrane (TM) protein, which is thought to be a member of the secretin receptor family. It is synthesized by neurons of the CNS and likely is a negative regulator of angiogenesis. BAI3 is 1498 amino acids in size. It contains three distinct regions: an N-terminal extracellular domain (ECD) (aa25-883), a 7-TM segment, and a C-terminal cytoplasmic region. The ECD contains four antiangiogenic TSP type 1 repeat (aa296-508), and one GSP domain (aa 816-867) that is likely used to cleave the ECD from the membrane-bound receptor. There is one altermate splice form that shows a deletion of aa 643-665. Over aa 25-880, human BAI3 shares 98% aa identity with mouse BAI3. BAI3 has been reported primarily in the brain, but is also localized to lung, testis, and pancreas. It might be involved in angiogenesis inhibition and suppression of glioblastoma.
Alternative Names: Brain-Specific Angiogenesis Inhibitor 3, BAI3, KIAA0550

Molecular Weight: 97.51 kDa

UniProt: O60242