CRTAM Protein (AA 18-286) (His tag)

Catalog No. ABIN1096449

Product Details

Target: CRTAM (Cytotoxic and Regulatory T Cell Molecule (CRTAM))

Protein Type: Recombinant

Protein Characteristics: AA 18-286

Origin: Human

Source: Human Cells

Purification tag / Conjugate: This CRTAM protein is labelled with His tag.

Purpose: Recombinant Human CRTAMV/CD355 (C-6His)

Sequence: SLTNHTETIT VEEGQTLTLK CVTSLRKNSS LQWLTPSGFT IFLNEYPVLK NSKYQLLHHS ANQLSITVPN VTLQDEGVYK CLHYSDSVST KEVKVIVLAT PFKPILEASV IRKQNGEEHV VLMCSTMRSK PPPQITWLLG NSMEVSGGTL HEFETDGKKC NTTSTLIIHT YGKNSTVDCI IRHRGLQGRK LVAPFRFEDL VTDEETASDA LERNSLSSQD PQQPTSTVSV TEDSSTSEID KEEKEQTTQD PDLTTEANPQ YLGLARKKSV DHHHHHH

Characteristics: Recombinant Human Cytotoxic and Regulatory T-Cell Molecule/CRTAM is produced with our mammalian expression system in human cells. The target protein is expressed with sequence (Ser18-Ser286) of Human CRTAM fused with a polyhistidine tag at the C-terminus.

Purity: > 95 % as determined by reducing SDS-PAGE.

Sterility: 0.2 μm filtered

Endotoxin Level: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Buffer: Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.2.

Handling Advice: Always centrifuge tubes before opening. Do not mix by vortex or pipetting.

Storage: 4 °C/-20 °C/-80 °C

Storage Comment: Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7°C for 2-7 days.
Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Expiry Date: 3 months

Target Details

Target: CRTAM (Cytotoxic and Regulatory T Cell Molecule (CRTAM))

Alternative Name: crtam (CRTAM Products)

Synonyms: TAD Protein, cTADS Protein, MGC82176 Protein, CRTAM Protein, CD355 Protein, cytotoxic and regulatory T-cell molecule Protein, cytotoxic and regulatory T-cell molecule L homeolog Protein, cytotoxic and regulatory T cell molecule Protein, CRTAM Protein, crtam.L Protein, Crtam Protein

Sub Type: Fusionprotein

Background: Cytotoxic and Regulatory T-Cell Molecule (CRTAM) is a member of Nectin family under the immunoglobulin superfamily that is expressed by activated CD8+ and NK T cells. CRTAM is found in spleen, thymus, small intestine, peripheral blood, and it is highly expressed by Purkinje cells of the cerebellum. CRTAM is a type I transmembrane glycoprotein containing one Ig-like C2-type domain and one Ig-like V-type domain in its extracellular domain, while its cytoplasmic region shows a potential class I PDZ domain. CRTAM is expressed as a homodimer on the cell surface but does not show homotypic binding in trans. The high affinity of CRTAM/IGSF4 adhesion allows CRTAM to disrupt IGSF4 homotypic interactions. IGSF4 and T cell receptor coengagement of CD8+ cells expressiong CRTAM induces increased IFNgamma or IL-22 production.
Alternative Names: Cytotoxic and Regulatory T-Cell Molecule, Class-I MHC-Restricted T-Cell-Associated Molecule, CD355, CRTAM

Molecular Weight: 30.99 kDa

UniProt: O95727

Pathways: Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Activated T Cell Proliferation