CRTAM Protein (AA 18-287) (His tag)

Catalog No. ABIN7274416

Product Details

Target: CRTAM (Cytotoxic and Regulatory T Cell Molecule (CRTAM))

Protein Type: Recombinant

Protein Characteristics: AA 18-287

Origin: Human

Source: HEK-293 Cells

Purification tag / Conjugate: This CRTAM protein is labelled with His tag.

Purpose: Human CRTAM Protein

Sequence: Ser18-Gly287

Characteristics: Recombinant Human CRTAM Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Ser18-Gly287.

Purity: > 95 % as determined by Tris-Bis PAGE

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1EU per μg by the LAL method.

Biological Activity Comment: Immobilized Human CRTAM, His Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Anti-CRTAM Antibody, hFc Tag with the EC50 of 57.3ng/ml determined by ELISA. See testing image for detail.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: CRTAM (Cytotoxic and Regulatory T Cell Molecule (CRTAM))

Alternative Name: CRTAM (CRTAM Products)

Synonyms: TAD Protein, cTADS Protein, MGC82176 Protein, CRTAM Protein, CD355 Protein, cytotoxic and regulatory T-cell molecule Protein, cytotoxic and regulatory T-cell molecule L homeolog Protein, cytotoxic and regulatory T cell molecule Protein, CRTAM Protein, crtam.L Protein, Crtam Protein

Background: Class-I Restricted T Cell-Associated Molecule (CRTAM) is a protein that is expressed after T cell activation. The interaction of CRTAM with its ligand, nectin-like 2 (Necl2), is required for the efficient production of IL-17, IL-22, and IFNγ by murine CD4 T cells, and it plays a role in optimal CD8 T and NK cell cytotoxicity. CRTAM promotes the pro-inflammatory cytokine profile, therefore, it may take part in the immunopathology of autoimmune diseases such as diabetes type 1 or colitis.

Molecular Weight: 31.1 kDa. Due to glycosylation, the protein migrates to 55-70 kDa based on Tris-Bis PAGE result.

Pathways: Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Activated T Cell Proliferation