HEPACAM Protein (AA 34-240) (His tag)

Catalog No. ABIN1096816

Product Details

Target: HEPACAM (Hepatic and Glial Cell Adhesion Molecule (HEPACAM))

Protein Type: Recombinant

Protein Characteristics: AA 34-240

Origin: Human

Source: Human Cells

Purification tag / Conjugate: This HEPACAM protein is labelled with His tag.

Purpose: Recombinant Human Hepatocyte Cell Adhesion Molecule/HepaCAM (C-6His)

Sequence: VNITSPVRLI HGTVGKSALL SVQYSSTSSD RPVVKWQLKR DKPVTVVQSI GTEVIGTLRP DYRDRIRLFE NGSLLLSDLQ LADEGTYEVE ISITDDTFTG EKTINLTVDV PISRPQVLVA STTVLELSEA FTLNCSHENG TKPSYTWLKD GKPLLNDSRM LLSPDQKVLT ITRVLMEDDD LYSCMVENPI SQGRSLPVKI TVYRRSSVDH HHHHH

Characteristics: Recombinant Human Hepatocyte Cell Adhesion Molecule/HEPACAM produced by transfected human cellss is a secreted protein with sequence (Val34-Ser240) of Human HEPACAM fused with a polyhistidine tag at the C-terminus.

Purity: > 95 % as determined by reducing SDS-PAGE.

Sterility: 0.2 μm filtered

Endotoxin Level: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Buffer: Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.2.

Handling Advice: Always centrifuge tubes before opening. Do not mix by vortex or pipetting.

Storage: 4 °C/-20 °C/-80 °C

Storage Comment: Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7°C for 2-7 days.
Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Expiry Date: 3 months

Target Details

Target: HEPACAM (Hepatic and Glial Cell Adhesion Molecule (HEPACAM))

Alternative Name: hepacam (HEPACAM Products)

Synonyms: GlialCAM Protein, MLC2A Protein, MLC2B Protein, 2900042E01Rik Protein, Hepn1 Protein, hepatic and glial cell adhesion molecule Protein, hepatocyte cell adhesion molecule Protein, HEPACAM Protein, Hepacam Protein

Sub Type: Fusionprotein

Background: Hepatocyte Cell Adhesion Molecule (HEPACAM) is a single-pass type I membrane protein that localizes to the cytoplasmic side of the cell membrane. HEPACAM includes a signal sequence (amino acid 1-33), an extracellular region (amino acid 34-240) with one Ig-like C2-type domain and one Ig-like V-type domain, a transmembrane segment (amino acid 241-261), and a cytoplasmic domain (amino acid 262 - 416). The cytoplasmic domain plays an important role in regulation of cell-matrix adhesion and cell motility. HEPACAM acts as a homodimer and dimer formation occurs predominantly through cis interactions on the cell surface. HEPACAM is involved in cell motility and cell-matrix interactions. The expression of this gene is down-regulated or undetectable in many cancer cell lines, so this may be a tumor suppressor gene.
Alternative Names: Hepatocyte Cell Adhesion Molecule, Protein HepaCAM, HEPACAM

Molecular Weight: 24.09 kDa

UniProt: Q14CZ8