IL16 Protein

Catalog No. ABIN1096900

Product Details

Target: IL16 (Interleukin 16 (IL16))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: Escherichia coli (E. coli)

Application: Functional Studies (Func)

Purpose: Recombinant Human Interleukin-16/IL-16

Sequence: MPDLNSSTDS AASASAASDV SVESTAEATV CTVTLEKMSA GLGFSLEGGK GSLHGDKPLT INRIFKGAAS EQSETVQPGD EILQLGGTAM QGLTRFEAWN IIKALPDGPV TIVIRRKSLQ SKETTAAGDS

Characteristics: Recombinant Human Interleukin-16/IL-16 produced in E. coli is a single non-glycosylated polypeptide chain containing 130 amino acids with a molecular mass of 13.4 kDa.

Purity: > 95 % as determined by reducing SDS-PAGE.

Sterility: 0.2 μm filtered

Endotoxin Level: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test

Application Details

Comment:

Biological activity: Specific Activity is greater than 1.0 x 104 IU/mg as determined by the ability of Recombinant IL-16 to chemoattract human CD4+ T lymphocytes using a concentration range of 10.0-100.0 ng/ml

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Buffer: Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.0.

Handling Advice: Always centrifuge tubes before opening. Do not mix by vortex or pipetting.

Storage: 4 °C/-20 °C/-80 °C

Storage Comment: Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7°C for 2-7 days.
Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Expiry Date: 3 months

Target Details

Target: IL16 (Interleukin 16 (IL16))

Alternative Name: il-16 (IL16 Products)

Synonyms: IL16 Protein, LCF Protein, NIL16 Protein, PRIL16 Protein, prIL-16 Protein, mKIAA4048 Protein, IL-16 Protein, interleukin 16 Protein, IL16 Protein, Il16 Protein

Background: Interleukin-16 (IL-16) is a CD8+ T cell-derived cytokine that induces chemotaxis of CD4+ T cells and CD4+ monocytes and eosinophils. Analysis by gel filtration suggests that, under physiological conditions, human IL-16 exists predominantly as a noncovalently linked multimer, but that some IL-16 may exist as a monomer. However, only the multimeric form appears to possess chemotactic activity, suggesting that receptor cross-linking may be required for activity. IL-16 also induces expression of IL-2 receptor (IL-2R) and MHC class II molecules on CD4+ T cells. Human and murine IL-16 show significant cross-species reactivity.ReferencesFeng M, et al. C16 peptide shown to prevent leukocyte infiltration and alleviate detrimental inflammation in acute allergic encephalomyelitis model.PMID: 23352465http://www.ncbi.nlm.nih.gov/pubmed/23352465
Alternative Names: Pro-Interleukin-16, Interleukin-16, IL-16, Lymphocyte Chemoattractant Factor, LCF, IL16

Molecular Weight: 13.4 kDa