IL-8 Protein

Catalog No. ABIN1305113

Product Details

Target: IL-8 (IL8) (Interleukin 8 (IL8))

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Application: ELISA, Functional Studies (Func), Intracellular Flow Cytometry (ICFC), Blocking Reagent (BR)

Brand: BD Pharmingen™

Specificity: Representative Biological Activity: Measured Calcium flux using lysed whole blood. The ED50 for these effects is typically 0.15 - 0.30 µg/ml or 0.1 -0.5 ng/ml respectively.

Characteristics: Purified human IL-8 was found to be > 98% pure as measured by SDS-PAGE analysis and an absorbance assay based on the Beers-Lambert law. Recombinant human IL-8 is a lyophilized aqueous buffered solution containing 50 µg bovine serum albumin per 1µg recombinant human IL-8. The product contains no preservatives.

Purity: ≥ 98 %

Sterility: 0.22 μm filtered

Endotoxin Level: The endotoxin level is ≤ 0.1 ng endotoxin per µg human IL-8 as measured using a chromogenic LAL assay.

Application Details

Application Notes: ELISA Standard: Recombinant human IL-8 is useful as a quantitative standard for measuring human IL-8 protein levels in an IL-8 specific sandwich ELISA with the purified G265-5 antibody (Cat. No. 554716) as a capture antibody and the biotinylated G265-8 (Cat. No. 554718) as the detection antibody. To obtain linear standard curves, doubling dilutions of this human IL-8 standard from 500-4 pg/ml should be included in each ELISA plate.
Note: This ELISA pair is recommended primarily for measuring cytokine from experimental cell culture systems. These ELISA reagents are not recommended for assaying serum or plasma samples. For measuring human IL-8 in serum or plasma the BD OptEIA™ human IL-8 ELISA Set (Cat. No. 555244) and OptEIA™ human IL-8 ELISA Kit (Cat. No. 550999) are specially formulated and recommended.

Ligand Blocking Control for Immunofluorescent Staining of Cytokines: Recombinant human IL-8 can be used as a blocking control to demonstrate specificity of IL-8 staining by the directly conjugated G265-8 antibody (Cat. No. 554720). The intracellular cytokine staining technique and use of blocking controls are described in detail by C. Prussin and D. Metcalfe.
Note: Carrier proteins should be pre-screened for possible effects in an appropriate experimental system. Carrier proteins may effect experimental results due to toxicity, high endotoxin levels or possible blocking activity.

Comment:

BD Pharmingen™ Recombinant Human IL-8

Restrictions: For Research Use only

Handling

Format: Lyophilized

Preservative: Azide free

Handling Advice: This preparation contains no preservatives, thus it should be handled under aseptic conditions. Store product at -80°C prior to reconstitution, or for long term storage of stock solutions.The product can be reconstituted in sterile neutral buffer containing carrier protein such as human or bovine serum albumin, aliquoted and stored at -80°C. For in vitro biological assay use, we recommend carrier-protein concentrations of 0.5 -1 mg/ml. For use as an ELISA standard we recommend carrier-protein concentrations of 5 - 10 mg/ml.
Note: Failure to add carrier protein or store at indicated temperatures may result in a loss of activity.

Storage: -80 °C

Storage Comment: This preparation contains no preservatives, thus it should be handled under aseptic conditions. Store product at -80°C prior to reconstitution, or for long term storage of stock solutions.The product can be reconstituted in sterile neutral buffer containing carrier protein* such as human or bovine serum albumin, aliquoted and stored at -80°C at a minimum concentration of 25ug/ml for six months. For in vitro biological assay use, we recommend carrier-protein concentrations of 0.5 -1 mg/ml. For use as an ELISA standard we recommend carrier-protein concentrations of 5 - 10 mg/ml. NOTE: Failure to add carrier protein or store at indicated temperatures may result in a loss of activity.

References

Prussin, Metcalfe: "Detection of intracytoplasmic cytokine using flow cytometry and directly conjugated anti-cytokine antibodies." in: Journal of immunological methods, Vol. 188, Issue 1, pp. 117-28, (1996) (PubMed).

Matsushima, Oppenheim: "Interleukin 8 and MCAF: novel inflammatory cytokines inducible by IL 1 and TNF." in: Cytokine, Vol. 1, Issue 1, pp. 2-13, (1991) (PubMed).

Target Details

Target: IL-8 (IL8) (Interleukin 8 (IL8))

Alternative Name: IL-8 (IL8 Products)

Synonyms: CXCL8 Protein, GCP-1 Protein, GCP1 Protein, LECT Protein, LUCT Protein, LYNAP Protein, MDNCF Protein, MONAP Protein, NAF Protein, NAP-1 Protein, NAP1 Protein, IL-8 Protein, AMCF-I Protein, il8 Protein, IL8 Protein, si:dkey-151b16.2 Protein, cxcli2 Protein, cxcl8 Protein, gcp-1 Protein, il-8 Protein, lynap Protein, mdncf Protein, monap Protein, nap-1 Protein, C-X-C motif chemokine ligand 8 Protein, interleukin 8 Protein, chemokine (C-X-C motif) ligand 8a Protein, chemokine (C-X-C motif) ligand 1-like Protein, chemokine (C-X-C motif) ligand 8 L homeolog Protein, CXCL8 Protein, il8 Protein, cxcl8a Protein, IL8 Protein, Cxcl8 Protein, LOC422654 Protein, cxcl8.L Protein

Background: Interleukin-8 (IL-8) is an 8 kD protein containing 72 amino acid residues. It promotes neutrophil chemotaxis and degranulation. The 72 amino acid IL-8 is the predominant form secreted by monocytes and lymphocytes.

Pathways: TLR Signaling, Cellular Response to Molecule of Bacterial Origin, Regulation of G-Protein Coupled Receptor Protein Signaling, ER-Nucleus Signaling, Hepatitis C, Autophagy