PNKP Protein (AA 1-521) (GST tag)
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- Target See all PNKP Proteins
- PNKP (Polynucleotide Kinase 3'-Phosphatase (PNKP))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-521
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Origin
- Human
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Source
- Wheat germ
- Purification tag / Conjugate
- This PNKP protein is labelled with GST tag.
- Application
- ELISA, Western Blotting (WB), Affinity Purification (AP), Antibody Array (AA)
- Purpose
- PNKP (Human) Recombinant Protein (P01)
- Sequence
- MGEVEAPGRL WLESPPGGAP PIFLPSDGQA LVLGRGPLTQ VTDRKCSRTQ VELVADPETR TVAVKQLGVN PSTTGTQELK PGLEGSLGVG DTLYLVNGLH PLTLRWEETR TPESQPDTPP GTPLVSQDEK RDAELPKKRM RKSNPGWENL EKLLVFTAAG VKPQGKVAGF DLDGTLITTR SGKVFPTGPS DWRILYPEIP RKLRELEAEG YKLVIFTNQM SIGRGKLPAE EFKAKVEAVV EKLGVPFQVL VATHAGLYRK PVTGMWDHLQ EQANDGTPIS IGDSIFVGDA AGRPANWAPG RKKKDFSCAD RLFALNLGLP FATPEEFFLK WPAAGFELPA FDPRTVSRSG PLCLPESRAL LSASPEVVVA VGFPGAGKST FLKKHLVSAG YVHVNRDTLG SWQRCVTTCE TALKQGKRVA IDNTNPDAAS RARYVQCARA AGVPCRCFLF TATLEQARHN NRFREMTDSS HIPVSDMVMY GYRKQFEAPT LAEGFSAILE IPFRLWVEPR LGRLYCQFSE G
- Characteristics
- Human PNKP full-length ORF ( NP_009185.2, 1 a.a. - 521 a.a.) recombinant protein with GST-tag at N-terminal.
- Purification
- in vitro wheat germ expression system
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Polynucleotide Kinase 3'-Phosphatase (PNKP) (AA 422-521) protein (GST tag)
PNKP Origin: Human Host: Wheat germ Recombinant ELISA, WB, AP, AA
Polynucleotide Kinase 3'-Phosphatase (PNKP) (AA 1-521) protein (Strep Tag)Crystallography grade PNKP Origin: Human Host: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, SDS
Polynucleotide Kinase 3'-Phosphatase (PNKP) (AA 1-522) protein (Strep Tag)Crystallography grade PNKP Origin: Mouse Host: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, SDS
Polynucleotide Kinase 3'-Phosphatase (PNKP) (AA 1-521), (full length) protein (His-SUMO Tag)PNKP Origin: Human Host: Escherichia coli (E. coli) Recombinant > 90 % SDS
Polynucleotide Kinase 3'-Phosphatase (PNKP) protein (Myc-DYKDDDDK Tag)PNKP Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Comment
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Preparation method: in vitro, wheat germ expression system
Product Quality tested by: 12.5% SDS-PAGE Stained with Coomassie Blue. - Restrictions
- For Research Use only
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- Buffer
- 50 mM Tris-HCI, 10 mM reduced Glutathione, pH =8.0 in the elution buffer.
- Handling Advice
- Aliquot to avoid repeated freezing and thawing.
- Storage
- -80 °C
- Storage Comment
- Best use within three months from the date of receipt of this protein.
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- Target
- PNKP (Polynucleotide Kinase 3'-Phosphatase (PNKP))
- Alternative Name
- PNKP (PNKP Products)
- Synonyms
- EIEE10 Protein, MCSZ Protein, PNK Protein, Tb06.28P18.320 Protein, 21.m03011 Protein, 1810009G08Rik Protein, polynucleotide kinase 3'-phosphatase Protein, hypothetical protein Protein, polynucleotide kinase 3'- phosphatase Protein, PNKP Protein, Pnkp Protein, Tc00.1047053507017.50 Protein, Tc00.1047053505807.190 Protein, Tb927.6.1580 Protein, BBOV_IV000690 Protein, CC1G_05202 Protein, PGTG_20262 Protein, PGTG_18987 Protein, pnkp.S Protein, pnkp Protein
- Background
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Full Gene Name: polynucleotide kinase 3'-phosphatase
Synonyms: PNK - Gene ID
- 11284
- NCBI Accession
- NM_007254
- Pathways
- DNA Damage Repair, Nucleotide Phosphorylation
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