PNKP Protein (AA 1-521, full length) (His-SUMO Tag)
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- Target See all PNKP Proteins
- PNKP (Polynucleotide Kinase 3'-Phosphatase (PNKP))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-521, full length
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This PNKP protein is labelled with His-SUMO Tag.
- Application
- SDS-PAGE (SDS)
- Sequence
- MGEVEAPGRL WLESPPGGAP PIFLPSDGQA LVLGRGPLTQ VTDRKCSRTQ VELVADPETR TVAVKQLGVN PSTTGTQELK PGLEGSLGVG DTLYLVNGLH PLTLRWEETR TPESQPDTPP GTPLVSQDEK RDAELPKKRM RKSNPGWENL EKLLVFTAAG VKPQGKVAGF DLDGTLITTR SGKVFPTGPS DWRILYPEIP RKLRELEAEG YKLVIFTNQM SIGRGKLPAE EFKAKVEAVV EKLGVPFQVL VATHAGLYRK PVTGMWDHLQ EQANDGTPIS IGDSIFVGDA AGRPANWAPG RKKKDFSCAD RLFALNLGLP FATPEEFFLK WPAAGFELPA FDPRTVSRSG PLCLPESRAL LSASPEVVVA VGFPGAGKST FLKKHLVSAG YVHVNRDTLG SWQRCVTTCE TALKQGKRVA IDNTNPDAAS RARYVQCARA AGVPCRCFLF TATLEQARHN NRFREMTDSS HIPVSDMVMY GYRKQFEAPT LAEGFSAILE IPFRLWVEPR LGRLYCQFSE G
- Purification
- SDS-PAGE
- Purity
- > 90 %
- Top Product
- Discover our top product PNKP Protein
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Polynucleotide Kinase 3'-Phosphatase (PNKP) (AA 1-521) protein (GST tag)
PNKP Origin: Human Host: Wheat germ Recombinant ELISA, WB, AP, AA
Polynucleotide Kinase 3'-Phosphatase (PNKP) (AA 422-521) protein (GST tag)PNKP Origin: Human Host: Wheat germ Recombinant ELISA, WB, AP, AA
Polynucleotide Kinase 3'-Phosphatase (PNKP) (AA 1-521) protein (Strep Tag)Crystallography grade PNKP Origin: Human Host: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, SDS
Polynucleotide Kinase 3'-Phosphatase (PNKP) (AA 1-522) protein (Strep Tag)Crystallography grade PNKP Origin: Mouse Host: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, SDS
Polynucleotide Kinase 3'-Phosphatase (PNKP) protein (Myc-DYKDDDDK Tag)PNKP Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 0.1-2 mg/mL
- Buffer
- 20 mM Tris-HCl based buffer, pH 8.0
- Storage
- -80 °C,4 °C,-20 °C
- Storage Comment
- Store at -20°C, for extended storage, conserve at -20°C or -80°C. Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
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- Target
- PNKP (Polynucleotide Kinase 3'-Phosphatase (PNKP))
- Alternative Name
- PNKP (PNKP Products)
- Synonyms
- EIEE10 Protein, MCSZ Protein, PNK Protein, Tb06.28P18.320 Protein, 21.m03011 Protein, 1810009G08Rik Protein, polynucleotide kinase 3'-phosphatase Protein, hypothetical protein Protein, polynucleotide kinase 3'- phosphatase Protein, PNKP Protein, Pnkp Protein, Tc00.1047053507017.50 Protein, Tc00.1047053505807.190 Protein, Tb927.6.1580 Protein, BBOV_IV000690 Protein, CC1G_05202 Protein, PGTG_20262 Protein, PGTG_18987 Protein, pnkp.S Protein, pnkp Protein
- Background
- Plays a key role in the repair of DNA damage, functioning as part of both the non-homologous end-joining (NHEJ) and base excision repair (BER) pathways. Through its two catalytic activities, PNK ensures that DNA termini are compatible with extension and ligation by either roving 3'-phosphates from, or by phosphorylating 5'-hydroxyl groups on, the ribose sugar of the DNA backbone.
- Molecular Weight
- 73 kDa
- UniProt
- Q96T60
- Pathways
- DNA Damage Repair, Nucleotide Phosphorylation
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