POLH Protein (AA 1-414) (GST tag)
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- Target See all POLH Proteins
- POLH (Polymerase (DNA Directed), eta (POLH))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-414
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Origin
- Human
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Source
- Wheat germ
- Purification tag / Conjugate
- This POLH protein is labelled with GST tag.
- Application
- Western Blotting (WB), ELISA, Affinity Purification (AP), Antibody Array (AA)
- Purpose
- POLH (Human) Recombinant Protein (P01)
- Sequence
- MATGQDRVVA LVDMDCFFVQ VEQRQNPHLR NKPCAVVQYK SWKGGGIIAV SYEARAFGVT RSMWADDAKK LCPDLLLAQV RESRGKANLT KYREASVEVM EIMSRFAVIE RASIDEAYVD LTSAVQERLQ KLQGQPISAD LLPSTYIEGL PQGPTTAEET VQKEGMRKQG LFQWLDSLQI DNLTSPDLQL TVGAVIVEEM RAAIERETGF QCSAGISHNK VLAKLACGLN KPNRQTLVSH GSVPQLFSQM PIRKIRSLGG KLGASVIEIL GIEYMGELTQ FTESQLQSHF GEKNGSWLYA MCRGIEHDPV KPRQLPKTIG CSKNFPGKTA LATREQVQWW LLQLAQELEE RLTKDRNDND RVATQLVVSI RVQGDKRLSS LRRCCALTRY DAHKMSHDAF TVIKNCNTSG IQTE
- Characteristics
- Human POLH full-length ORF ( AAH15742, 1 a.a. - 414 a.a.) recombinant protein with GST-tag at N-terminal.
- Purification
- in vitro wheat germ expression system
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Polymerase (DNA Directed), eta (POLH) (AA 1-713) protein (Strep Tag)
Crystallography grade POLH Origin: Human Host: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, ELISA, SDS
Polymerase (DNA Directed), eta (POLH) (AA 1-694) protein (Strep Tag)Crystallography grade POLH Origin: Mouse Host: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, ELISA, SDS
Polymerase (DNA Directed), eta (POLH) protein (Myc-DYKDDDDK Tag)POLH Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Comment
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Preparation method: in vitro, wheat germ expression system
Product Quality tested by: 12.5% SDS-PAGE Stained with Coomassie Blue. - Restrictions
- For Research Use only
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- Buffer
- 50 mM Tris-HCI, 10 mM reduced Glutathione, pH =8.0 in the elution buffer.
- Handling Advice
- Aliquot to avoid repeated freezing and thawing.
- Storage
- -80 °C
- Storage Comment
- Best use within three months from the date of receipt of this protein.
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- Target
- POLH (Polymerase (DNA Directed), eta (POLH))
- Alternative Name
- POLH (POLH Products)
- Synonyms
- POLH Protein, fc50a12 Protein, wu:fc50a12 Protein, zgc:136881 Protein, zgc:152642 Protein, RAD30 Protein, RAD30A Protein, XP-V Protein, XPV Protein, polymerase (DNA directed), eta S homeolog Protein, DNA polymerase eta Protein, polymerase (DNA directed), eta Protein, putative DNA polymerase eta Protein, polymerase (DNA directed), eta (RAD 30 related) Protein, polh.S Protein, POLH Protein, polh Protein, Tb10.70.6190 Protein, LINJ_21_0690 Protein, Polh Protein, LOC100443019 Protein
- Background
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Full Gene Name: polymerase (DNA directed), eta
Synonyms: FLJ16395,FLJ21978,RAD30,RAD30A,XP-V,XPV - Gene ID
- 5429
- Pathways
- DNA Damage Repair
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