PRMT5 Protein (His tag,DYKDDDDK Tag)

Catalog No. ABIN7317668

Product Details

Target: PRMT5 (Protein Arginine Methyltransferase 5 (PRMT5))

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Purification tag / Conjugate: This PRMT5 protein is labelled with His tag,DYKDDDDK Tag.

Purpose: Recombinant Human PRMT5/SKB1 Protein (His & FLAG Tag)

Sequence: Ala 2-Leu 637

Characteristics: A DNA sequence encoding the human PRMT5 (NP_006100.2) (Ala 2-Leu 637) was expressed, fused with a polyhistidine tag at the C-terminus and a Flag tag at the N-terminus.

Purity: > 85 % as determined by reducing SDS-PAGE.

Endotoxin Level: < 1.0 EU per μg as determined by the LAL method.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from sterile 50 mM Tris, 100 mM NaCl, pH 8.0, 0.5 mM EDTA, 0.5PMSF, 0.5 mM, TCEP, 25 % glycerol

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Target Details

Target: PRMT5 (Protein Arginine Methyltransferase 5 (PRMT5))

Alternative Name: PRMT5/SKB1 (PRMT5 Products)

Synonyms: HRMT1L5 Protein, IBP72 Protein, JBP1 Protein, SKB1 Protein, SKB1Hs Protein, Jbp1 Protein, Skb1 Protein, si:zc14a17.2 Protein, skb1 Protein, wu:fb95f10 Protein, zgc:110669 Protein, hsl7 Protein, DDBDRAFT_0187422 Protein, DDBDRAFT_0235403 Protein, DDB_0187422 Protein, DDB_0235403 Protein, NV18830 Protein, jbp1 Protein, ibp72 Protein, skb1hs Protein, hrmt1l5 Protein, protein arginine methyltransferase 5 Protein, protein arginine N-methyltransferase 5 Protein, protein arginine methyltransferase 5 L homeolog Protein, putative arginine N-methyltransferase, type II Protein, Skb1 family protein Protein, Protein arginine N-methyltransferase 5 Protein, PRMT5 Protein, Prmt5 Protein, prmt5 Protein, prmt5.L Protein, LOC100115766 Protein, prmt-5 Protein

Background:

Background: Methylation of arginine residues is a widespread post-translational modification of proteins catalyzed by a small family of PRMTs. The modification appears to regulate protein functions and interactions that affect gene regulation, signalling and subcellular localization of proteins and nucleic acids. Protein arginine methyltransferase 5 (PRMT5) is a member of the protein arginine N-methyltransferases (PRMT)family, and exists as at least homodimers and homotetramers, or homooligomers mediated by disulfide bonds and non-covalent association ubiquitously. PRMT5 specifically mediates the symmetrical dimethylation of arginine residues in the small nuclear ribonucleoproteins Sm D1 (SNRPD1) and Sm D3 (SNRPD3), and thus plays a role in the assembly and biogenesis of snRNP core particles. PRMT5 methylates histone H2A and H4 'Arg-3' during germ cell development, as well as histone H3 'Arg-8', which may repress transcription. PRMT5 also methylates SUPT5H and regulates its transcriptional elongation properties. Additionally, it is also suggested that PRMT5 negatively regulates cyclin E1 promoter activity and cellular proliferation.

Synonym: HRMT1L5,IBP72,JBP1,SKB1,SKB1Hs

Molecular Weight: 75 kDa

NCBI Accession: NP_006100

Pathways: Chromatin Binding, Regulation of Muscle Cell Differentiation, Ribonucleoprotein Complex Subunit Organization, Skeletal Muscle Fiber Development