RAF1 Protein (AA 1-648) (GST tag)
(1 reference)-
- Target See all RAF1 Proteins
- RAF1 (V-Raf-1 Murine Leukemia Viral Oncogene Homolog 1 (RAF1))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-648
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Origin
- Human
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Source
- Wheat germ
- Purification tag / Conjugate
- This RAF1 protein is labelled with GST tag.
- Application
- ELISA, Western Blotting (WB), Affinity Purification (AP), Antibody Array (AA)
- Purpose
- RAF1 (Human) Recombinant Protein (P01)
- Sequence
- MEHIQGAWKT ISNGFGFKDA VFDGSSCISP TIVQQFGYQR RASDDGKLTD PSKTSNTIRV FLPNKQRTVV NVRNGMSLHD CLMKALKVRG LQPECCAVFR LLHEHKGKKA RLDWNTDAAS LIGEELQVDF LDHVPLTTHN FARKTFLKLA FCDICQKFLL NGFRCQTCGY KFHEHCSTKV PTMCVDWSNI RQLLLFPNST IGDSGVPALP SLTMRRMRES VSRMPVSSQH RYSTPHAFTF NTSSPSSEGS LSQRQRSTST PNVHMVSTTL PVDSRMIEDA IRSHSESASP SALSSSPNNL SPTGWSQPKT PVPAQRERAP VSGTQEKNKI RPRGQRDSSY YWEIEASEVM LSTRIGSGSF GTVYKGKWHG DVAVKILKVV DPTPEQFQAF RNEVAVLRKT RHVNILLFMG YMTKDNLAIV TQWCEGSSLY KHLHVQETKF QMFQLIDIAR QTAQGMDYLH AKNIIHRDMK SNNIFLHEGL TVKIGDFGLA TVKSRWSGSQ QVEQPTGSVL WMAPEVIRMQ DNNPFSFQSD VYSYGIVLYE LMTGELPYSH INNRDQIIFM VGRGYASPDL SKLYKNCPKA MKRLVADCVK KVKEERPLFP QILSSIELLQ HSLPKINRSA SEPSLHRAAH TEDINACTLT TSPRLPVF
- Characteristics
- Human RAF1 full-length ORF ( NP_002871.1, 1 a.a. - 648 a.a.) recombinant protein with GST-tag at N-terminal.
- Purification
- in vitro wheat germ expression system
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V-Raf-1 Murine Leukemia Viral Oncogene Homolog 1 (RAF1) (AA 306-648), (Tyr340Asp-Mutant), (Tyr341Asp-Mutant) (Active) protein
RAF1 Origin: Human Host: Insect cells (Sf9) Recombinant SDS, Func Active
V-Raf-1 Murine Leukemia Viral Oncogene Homolog 1 (RAF1) (AA 1-130) protein (GST tag)RAF1 Origin: Human Host: Wheat germ Recombinant ELISA, WB, AP, AA
V-Raf-1 Murine Leukemia Viral Oncogene Homolog 1 (RAF1) (AA 1-648) protein (Strep Tag)Crystallography grade RAF1 Origin: Human Host: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, SDS
V-Raf-1 Murine Leukemia Viral Oncogene Homolog 1 (RAF1) (AA 1-648) protein (Strep Tag)Crystallography grade RAF1 Origin: Mouse Host: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, SDS
V-Raf-1 Murine Leukemia Viral Oncogene Homolog 1 (RAF1) (AA 1-648), (full length) protein (His-SUMO Tag)RAF1 Origin: Human Host: Escherichia coli (E. coli) Recombinant > 90 % SDS
V-Raf-1 Murine Leukemia Viral Oncogene Homolog 1 (RAF1) protein (Myc-DYKDDDDK Tag)RAF1 Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Comment
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Preparation method: in vitro, wheat germ expression system
Product Quality tested by: 12.5% SDS-PAGE Stained with Coomassie Blue. - Restrictions
- For Research Use only
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- Buffer
- 50 mM Tris-HCI, 10 mM reduced Glutathione, pH =8.0 in the elution buffer.
- Handling Advice
- Aliquot to avoid repeated freezing and thawing.
- Storage
- -80 °C
- Storage Comment
- Best use within three months from the date of receipt of this protein.
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: "NO-released zinc supports the simultaneous binding of Raf-1 and PKC? cysteine-rich domains to HINT1 protein at the mu-opioid receptor." in: Antioxidants & redox signaling, Vol. 14, Issue 12, pp. 2413-25, (2011) (PubMed).
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: "NO-released zinc supports the simultaneous binding of Raf-1 and PKC? cysteine-rich domains to HINT1 protein at the mu-opioid receptor." in: Antioxidants & redox signaling, Vol. 14, Issue 12, pp. 2413-25, (2011) (PubMed).
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- Target
- RAF1 (V-Raf-1 Murine Leukemia Viral Oncogene Homolog 1 (RAF1))
- Alternative Name
- RAF1 (RAF1 Products)
- Synonyms
- CRAF Protein, NS5 Protein, Raf-1 Protein, c-Raf Protein, c-raf Protein, craf Protein, raf1 Protein, RAF1 Protein, 6430402F14Rik Protein, AA990557 Protein, BB129353 Protein, Craf1 Protein, D830050J10Rik Protein, v-Raf Protein, raf Protein, cRaf Protein, Raf-1 proto-oncogene, serine/threonine kinase Protein, Raf-1 proto-oncogene, serine/threonine kinase a Protein, v-raf-leukemia viral oncogene 1 Protein, Raf-1 proto-oncogene, serine/threonine kinase S homeolog Protein, RAF1 Protein, Raf1 Protein, raf1a Protein, raf1 Protein, raf1.S Protein
- Background
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Full Gene Name: v-raf-1 murine leukemia viral oncogene homolog 1
Synonyms: CRAF,NS5,Raf-1,c-Raf - Gene ID
- 5894
- NCBI Accession
- NM_002880
- Pathways
- MAPK Signaling, RTK Signaling, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, cAMP Metabolic Process, Stem Cell Maintenance, Hepatitis C, Autophagy, Signaling of Hepatocyte Growth Factor Receptor, VEGF Signaling, BCR Signaling
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