SMAD4 Protein (AA 1-552) (GST tag)
(1 reference)-
- Target See all SMAD4 Proteins
- SMAD4 (SMAD Family Member 4 (SMAD4))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-552
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Origin
- Human
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Source
- Wheat germ
- Purification tag / Conjugate
- This SMAD4 protein is labelled with GST tag.
- Application
- Western Blotting (WB), ELISA, Affinity Purification (AP), Antibody Array (AA)
- Purpose
- SMAD4 (Human) Recombinant Protein (P01)
- Sequence
- MDNMSITNTP TSNDACLSIV HSLMCHRQGG ESETFAKRAI ESLVKKLKEK KDELDSLITA ITTSGAHPSK CVTIQRTLDG RLQVAGRKGF PHVIYARLWR WPDLHKNELK HVKYCQYAFD LKCDSVCVNP YHYERVVSPG IDLSGLTLQS NAPSSMMVKD EYVHDFEGQP SLSTEGHSIQ TIQHPPSNRA STETYSTPAL LAPSESNATS TANFPNIPVA STSQPASILG GSHSEGLLQI ASGPQPGQQQ NGFTGQPATY HHNSTTTWTG SRTAPYTPNL PHHQNGHLQH HPPMPPHPGH YWPVHNELAF QPPISNHPAP EYWCSIAYFE MDVQVGETFK VPSSCPIVTV DGYVDPSGGD RFCLGQLSNV HRTEAIERAR LHIGKGVQLE CKGEGDVWVR CLSDHAVFVQ SYYLDREAGR APGDAVHKIY PSAYIKVFDL RQCHRQMQQQ AATAQAAAAA QAAAVAGNIP GPGSVGGIAP AISLSAAAGI GVDDLRRLCI LRMSFVKGWG PDYPRQSIKE TPCWIEIHLH RALQLLDEVL HTMPIADPQP LD
- Characteristics
- Human SMAD4 full-length ORF ( AAH02379, 1 a.a. - 552 a.a.) recombinant protein with GST-tag at N-terminal.
- Purification
- in vitro wheat germ expression system
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SMAD Family Member 4 (SMAD4) (AA 13-227) protein (His tag)
SMAD4 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % by SDS-PAGE gel analyses WB, ELISA
SMAD Family Member 4 (SMAD4) (AA 56-165) protein (GST tag)SMAD4 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
SMAD Family Member 4 (SMAD4) (AA 1-552) protein (Strep Tag)Crystallography grade SMAD4 Origin: Human Host: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA
SMAD Family Member 4 (SMAD4) (AA 1-551) protein (Strep Tag)Crystallography grade SMAD4 Origin: Mouse Host: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA
SMAD Family Member 4 (SMAD4) protein (Myc-DYKDDDDK Tag)SMAD4 Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Comment
-
Preparation method: in vitro, wheat germ expression system
Product Quality tested by: 12.5% SDS-PAGE Stained with Coomassie Blue. - Restrictions
- For Research Use only
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- Buffer
- 50 mM Tris-HCI, 10 mM reduced Glutathione, pH =8.0 in the elution buffer.
- Handling Advice
- Aliquot to avoid repeated freezing and thawing.
- Storage
- -80 °C
- Storage Comment
- Best use within three months from the date of receipt of this protein.
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-
: "Transforming growth factor beta-induced reactivation of Epstein-Barr virus involves multiple Smad-binding elements cooperatively activating expression of the latent-lytic switch BZLF1 gene." in: Journal of virology, Vol. 85, Issue 15, pp. 7836-48, (2011) (PubMed).
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: "Transforming growth factor beta-induced reactivation of Epstein-Barr virus involves multiple Smad-binding elements cooperatively activating expression of the latent-lytic switch BZLF1 gene." in: Journal of virology, Vol. 85, Issue 15, pp. 7836-48, (2011) (PubMed).
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- Target
- SMAD4 (SMAD Family Member 4 (SMAD4))
- Alternative Name
- SMAD4 (SMAD4 Products)
- Synonyms
- smad4 Protein, DPC4 Protein, JIP Protein, MADH4 Protein, MYHRS Protein, AW743858 Protein, D18Wsu70e Protein, Madh4 Protein, XSmad4alpha Protein, Xsmad4 Protein, dpc4 Protein, jip Protein, madh4 Protein, xsmad4a Protein, SMAD family member 4 Protein, Smad4 protein Protein, SMAD family member 4, gene 1 L homeolog Protein, SMAD4 Protein, smad4 Protein, Smad4 Protein, smad4.1.L Protein
- Background
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Full Gene Name: SMAD family member 4
Synonyms: DPC4,JIP,MADH4 - Gene ID
- 4089
- Pathways
- Cell Division Cycle, Chromatin Binding, Autophagy
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