TRAIL Protein (AA 99-291, Extracellular Domain, Soluble) (His tag)

Catalog No. ABIN1344429

Product Details

Target: TRAIL (TNFSF10) (Tumor Necrosis Factor (Ligand) Superfamily, Member 10 (TNFSF10))

Protein Type: Recombinant

Biological Activity: Active

Protein Characteristics: Soluble, Extracellular Domain, AA 99-291

Origin: Human, Mouse

Source: Escherichia coli (E. coli)

Purification tag / Conjugate: This TRAIL protein is labelled with His tag.

Application: SDS-PAGE (SDS)

Brand: SuperKillerTRAIL™

Specificity: Binds to mouse and less potently to human TRAIL receptors.

Cross-Reactivity: Human, Mouse (Murine)

Characteristics: The extracellular domain of mouse TRAIL (aa 99-291) is fused at the N-terminus to a His-tag and a linker peptide. The active multimeric conformation is stabilized by an inserted mutation allowing an additional CC-bridge.

Purity: >95 % (SDS-PAGE)

Endotoxin Level: <0.01EU/μg purified protein (LAL test, Lonza).

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Induces apoptosis at concentrations of >10ng/ml as tested on human tumor cells. This effect can be efficiently blocked by recombinant mouse TRAIL receptors. This protein does not require a cross-linker (enhancer) for its potent biological activity.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: In 20 mM HEPES, pH 7.4, 300 mM NaCl, 0.01 % Tween 20, 1 % sucrose, 1 mM DTT.

Preservative: Dithiothreitol (DTT)

Precaution of Use: This product contains Dithiothreitol (DTT): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,-80 °C

Storage Comment: Short Term Storage: -20°C
Long Term Storage: -80°C
Once thawed it is recommended to prepare appropriate aliquots and to store them at -80°C. , Stable for at least 1 year after receipt when stored at -80°C.

Expiry Date: 12 months

Target Details

Target: TRAIL (TNFSF10) (Tumor Necrosis Factor (Ligand) Superfamily, Member 10 (TNFSF10))

Alternative Name: TRAIL (TNFSF10 Products)

Synonyms: APO2L Protein, Apo-2L Protein, CD253 Protein, TL2 Protein, TRAIL Protein, A330042I21Rik Protein, AI448571 Protein, APO-2L Protein, Ly81 Protein, Trail Protein, tl2 Protein, apo2l Protein, cd253 Protein, trail Protein, apo-2l Protein, trail1 Protein, xtrail1 Protein, tnfsf10l2 Protein, zgc:92320 Protein, TNLG6A Protein, TNF superfamily member 10 Protein, tumor necrosis factor (ligand) superfamily, member 10 Protein, tumor necrosis factor superfamily member 10 Protein, TNF superfamily member 10 S homeolog Protein, TNFSF10 Protein, Tnfsf10 Protein, tnfsf10.S Protein, tnfsf10 Protein

Background: TNF-related apoptosis-inducing ligand (TRAIL, Apo2L,CD253, TNFSF10) is a type II transmembrane protein of about 34 kDa. Like most members of the tumor necrosis factor (TNF) superfamily of cytokines TRAIL can be cleaved at the cell surface by metalloproteases to form a soluble molecule. Active TRAIL forms trimers and specifically binds to five distinct receptors: TRAIL-R1 (DR4, Apo2,CD261, TNFRSF10A), TRAIL-R2 (DR5, KILLER, TRICK2A,TRICK2B, CD262, TNFRSF10B), TRAIL-R3 (DcR1,LIT, TRID, CD263, TNFRSF10C), TRAIL-R4 (DcR2, TRUNDD, CD264, TNFRSF10D), and osteoprotegerin (OPG, OCIF, TNFRSF11B). Trimerized TRAIL triggers apoptosis upon ligation of cell surface TRAIL-R1 and/or TRAIL-R2 by inducing the formation of the so-called multiprotein death-inducing signaling complex (DISC).

Molecular Weight: ~24kDa

UniProt: P50592

Pathways: Apoptosis, Positive Regulation of Endopeptidase Activity